Overview
- External ontology
- jcm.grmd:1474 (source link unavailable) — BL BROTH WITH 2% NaCl
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1474
Composition
flowchart LR
CultureMech_015890["bl_broth_with_2_nacl"]
ing0_Lab_Lemco_powder__Oxoid_["Lab-Lemco powder (Oxoid)"]
CultureMech_015890 --> ing0_Lab_Lemco_powder__Oxoid_
ing1_Proteose_peptone_No__3__BD_Difco_["Proteose peptone No. 3 (BD-Difco)"]
CultureMech_015890 --> ing1_Proteose_peptone_No__3__BD_Difco_
ing2_Trypticase_peptone__BD_BBL_["Trypticase peptone (BD-BBL)"]
CultureMech_015890 --> ing2_Trypticase_peptone__BD_BBL_
ing3_Phytone_peptone__BD_BBL_["Phytone peptone (BD-BBL)"]
CultureMech_015890 --> ing3_Phytone_peptone__BD_BBL_
ing4_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_015890 --> ing4_Yeast_extract__BD_Difco_
ing5_NaCl["NaCl"]
CultureMech_015890 --> ing5_NaCl
ing6_Liver_extract__see_below_["Liver extract (see below)"]
CultureMech_015890 --> ing6_Liver_extract__see_below_
ing7_Glucose["Glucose"]
CultureMech_015890 --> ing7_Glucose
ing8_Soluble_starch["Soluble starch"]
CultureMech_015890 --> ing8_Soluble_starch
ing9_Solution_A__see_below_["Solution A (see below)"]
CultureMech_015890 --> ing9_Solution_A__see_below_
ing10_Solution_B__see_below_["Solution B (see below)"]
CultureMech_015890 --> ing10_Solution_B__see_below_
ing11_Tween_80["Tween 80"]
CultureMech_015890 --> ing11_Tween_80
ing12_5__L_Cysteine_HCl_H2O_solution["5% L-Cysteine·HCl·H2O solution"]
CultureMech_015890 --> ing12_5__L_Cysteine_HCl_H2O_solution
ing13_Distilled_water["Distilled water"]
CultureMech_015890 --> ing13_Distilled_water
Ingredients (14)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Lab-Lemco powder (Oxoid) | 3.0 G_PER_L | ||
| 2 | Proteose peptone No. 3 (BD-Difco) | 10.0 G_PER_L | ||
| 3 | Trypticase peptone (BD-BBL) | 5.0 G_PER_L | ||
| 4 | Phytone peptone (BD-BBL) | 3.0 G_PER_L | ||
| 5 | Yeast extract (BD-Difco) | 5.0 G_PER_L | ||
| 6 | NaCl | 20.0 G_PER_L | ||
| 7 | Liver extract (see below) | 150.0 ML_PER_L | ||
| 8 | Glucose | 10.0 G_PER_L | ||
| 9 | Soluble starch | 0.5 G_PER_L | ||
| 10 | Solution A (see below) | 10.0 ML_PER_L | ||
| 11 | Solution B (see below) | 5.0 ML_PER_L | ||
| 12 | Tween 80 | 1.0 G_PER_L | ||
| 13 | 5% L-Cysteine·HCl·H2O solution | 10.0 ML_PER_L | ||
| 14 | Distilled water | 825.0 ML_PER_L |
Preparation
- Adjust pH to 7.2.
- To prepare liver extract, put 10 g liver powder in 170 ml water, keep at 50 to 60C for 1 hr, boil for 5 min, adjust pH to 7.2 and filter.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- JCM_J1474_BL_BROTH_WITH_2_NaCl (CultureMech:015890)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-10-01T01:54:30.313734+00:00 | jcm-grmd-import | Scraped from JCM GRMD database | Direct import from JCM GRMD=1474. Ingredients captured verbatim. CHEBI/MediaIngredientMech enrichment and category (bacterial/archaea) review are downstream steps. |
| 2026-10-01T02:55:00+00:00 | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:015890 |
| 2026-10-01T03:00:53.998270+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 5bffe9f701f4ca774eb24b32d2d2856e24cf54540fb0fe77855d5d13b389b228 |