Overview
- External ontology
-
jcm.grmd:1474 (source link unavailable)
— BL BROTH WITH 2% NaCl
- Applications
-
- pH
- 7.2
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1474
Composition
flowchart LR
CultureMech_015890["bl_broth_with_2_nacl"]
ing0_Lab_Lemco_powder__Oxoid_["Lab-Lemco powder (Oxoid)"]
CultureMech_015890 --> ing0_Lab_Lemco_powder__Oxoid_
ing1_Proteose_peptone_No__3__BD_Difco_["Proteose peptone No. 3 (BD-Difco)"]
CultureMech_015890 --> ing1_Proteose_peptone_No__3__BD_Difco_
ing2_Trypticase_peptone__BD_BBL_["Trypticase peptone (BD-BBL)"]
CultureMech_015890 --> ing2_Trypticase_peptone__BD_BBL_
ing3_Phytone_peptone__BD_BBL_["Phytone peptone (BD-BBL)"]
CultureMech_015890 --> ing3_Phytone_peptone__BD_BBL_
ing4_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_015890 --> ing4_Yeast_extract__BD_Difco_
ing5_NaCl["NaCl"]
CultureMech_015890 --> ing5_NaCl
ing6_Liver_extract__see_below_["Liver extract (see below)"]
CultureMech_015890 --> ing6_Liver_extract__see_below_
ing7_Glucose["Glucose"]
CultureMech_015890 --> ing7_Glucose
ing8_Soluble_starch["Soluble starch"]
CultureMech_015890 --> ing8_Soluble_starch
ing9_Solution_A__see_below_["Solution A (see below)"]
CultureMech_015890 --> ing9_Solution_A__see_below_
ing10_Solution_B__see_below_["Solution B (see below)"]
CultureMech_015890 --> ing10_Solution_B__see_below_
ing11_Tween_80["Tween 80"]
CultureMech_015890 --> ing11_Tween_80
ing12_5__L_Cysteine_HCl_H2O_solution["5% L-Cysteine·HCl·H2O solution"]
CultureMech_015890 --> ing12_5__L_Cysteine_HCl_H2O_solution
ing13_Distilled_water["Distilled water"]
CultureMech_015890 --> ing13_Distilled_water
Ingredients (14)
| # |
Ingredient |
Concentration |
Ontology |
Notes |
| 1 |
Lab-Lemco powder (Oxoid)
|
3.0
G_PER_L
|
|
|
| 2 |
Proteose peptone No. 3 (BD-Difco)
|
10.0
G_PER_L
|
|
|
| 3 |
Trypticase peptone (BD-BBL)
|
5.0
G_PER_L
|
|
|
| 4 |
Phytone peptone (BD-BBL)
|
3.0
G_PER_L
|
|
|
| 5 |
Yeast extract (BD-Difco)
|
5.0
G_PER_L
|
|
|
| 6 |
NaCl
|
20.0
G_PER_L
|
|
|
| 7 |
Liver extract (see below)
|
150.0
ML_PER_L
|
|
|
| 8 |
Glucose
|
10.0
G_PER_L
|
|
|
| 9 |
Soluble starch
|
0.5
G_PER_L
|
|
|
| 10 |
Solution A (see below)
|
10.0
ML_PER_L
|
|
|
| 11 |
Solution B (see below)
|
5.0
ML_PER_L
|
|
|
| 12 |
Tween 80
|
1.0
G_PER_L
|
|
|
| 13 |
5% L-Cysteine·HCl·H2O solution
|
10.0
ML_PER_L
|
|
|
| 14 |
Distilled water
|
825.0
ML_PER_L
|
|
|
Preparation
- Adjust pH to 7.2.
- To prepare liver extract, put 10 g liver powder in 170 ml water, keep at 50 to 60C for 1 hr, boil for 5 min, adjust pH to 7.2 and filter.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|
| 2026-10-01T01:54:30.313734+00:00 |
jcm-grmd-import |
Scraped from JCM GRMD database |
Direct import from JCM GRMD=1474. Ingredients captured verbatim. CHEBI/MediaIngredientMech enrichment and category (bacterial/archaea) review are downstream steps. |
| 2026-10-01T02:55:00+00:00 |
culturemech-id-assigner-v1.0 |
Assigned CultureMech ID |
Assigned stable identifier: CultureMech:015890 |