Overview
- External ontology
- nbrc.medium:1407 (source link unavailable) — PPLO broth for Mycoplasma arginini
- Applications
-
- Microbial cultivation
- pH
- 7.1
- Notes
- Source: NBRC | Link: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1407
Composition
flowchart LR
CultureMech_015909["pplo_broth_for_mycoplasma_arginini_nbrc_1407"]
ing0_Bacto_PPLO_Broth_w_o_CV__Difco_["Bacto PPLO Broth w/o CV (Difco)"]
CultureMech_015909 --> ing0_Bacto_PPLO_Broth_w_o_CV__Difco_
ing1_Horse_serum["Horse serum"]
CultureMech_015909 --> ing1_Horse_serum
ing2_25__Yeast_extract_solution["25% Yeast extract solution"]
CultureMech_015909 --> ing2_25__Yeast_extract_solution
ing3_Phenol_red["Phenol red"]
CultureMech_015909 --> ing3_Phenol_red
CHEBI_31991["CHEBI:31991"]
ing3_Phenol_red -.-> CHEBI_31991
ing4_L_Arginine["L-Arginine"]
CultureMech_015909 --> ing4_L_Arginine
CHEBI_16467["CHEBI:16467"]
ing4_L_Arginine -.-> CHEBI_16467
ing5_L_Glutamine["L-Glutamine"]
CultureMech_015909 --> ing5_L_Glutamine
CHEBI_18050["CHEBI:18050"]
ing5_L_Glutamine -.-> CHEBI_18050
ing6_100X_MEM_Vitamins__Gibco_["100X MEM Vitamins (Gibco)"]
CultureMech_015909 --> ing6_100X_MEM_Vitamins__Gibco_
ing7_Agar__if_needed_["Agar (if needed)"]
CultureMech_015909 --> ing7_Agar__if_needed_
CHEBI_2509["CHEBI:2509"]
ing7_Agar__if_needed_ -.-> CHEBI_2509
ing8_Distilled_water["Distilled water"]
CultureMech_015909 --> ing8_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing8_Distilled_water -.-> CHEBI_15377
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Bacto PPLO Broth w/o CV (Difco) | 14.7 G_PER_L | ||
| 2 | Horse serum | 200 ML_PER_L | ||
| 3 | 25% Yeast extract solution | 100 ML_PER_L | ||
| 4 |
Phenol red
C19H14O5S 354.383 g/mol
|
20 MG_PER_L |
CHEBI:31991
phenol red
|
|
| 5 |
L-Arginine
C6H14N4O2 174.204 g/mol
|
2.1 G_PER_L |
CHEBI:16467
L-arginine
|
|
| 6 |
L-Glutamine
C5H10N2O3 146.146 g/mol
|
1.46 G_PER_L |
CHEBI:18050
L-glutamine
|
|
| 7 | 100X MEM Vitamins (Gibco) | 1 ML_PER_L | ||
| 8 | Agar (if needed) | 15 G_PER_L |
CHEBI:2509
agar
|
NBRC lists this component as optional when a solid medium is needed. |
| 9 |
Distilled water
H2O 18.015 g/mol
|
600 ML_PER_L |
CHEBI:15377
water
|
Preparation
- *Sterilize by filtration, and add to another component sterilized by autoclaving.
- **Boil 250 g of dried baker's yeast suspended in 1 L of distilled water for 30 min, remove yeast cells by centrifugation, and sterilize by filtration.
- ***Prepare 100 mM solution and sterilize by filtration.
- The anaerobic growth on plates was carried out in an anaerobic jar equipped with a CO2-generating gas pack (Mitsubishi Gas Chemical Company Inc., Tokyo, Japan).
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- pplo_broth_for_mycoplasma_arginini_nbrc_1407 (CultureMech:015909)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-10-02T05:20:01.508978+00:00 | nbrc-live-import | Scraped from NBRC catalogue | Direct import from NBRC Medium 1407. Ingredients captured verbatim from the live catalogue. |
| 2026-10-02T05:20:01.508978+00:00 | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:015909 |
| 2026-10-02T05:20:01.508978+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 60989300cf8ecf3f8b3cb6ef16c1a9b39c86bbd18f860e64001f248eeadba0cf |