pplo_broth_for_mycoplasma_arginini_nbrc_1407

CultureMech:015909 bacterial COMPLEX LIQUID
Original name: PPLO broth for Mycoplasma arginini

Overview

External ontology
nbrc.medium:1407 (source link unavailable) — PPLO broth for Mycoplasma arginini
Applications
  • Microbial cultivation
pH
7.1
Notes
Source: NBRC | Link: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1407

Composition

flowchart LR
    CultureMech_015909["pplo_broth_for_mycoplasma_arginini_nbrc_1407"]
    ing0_Bacto_PPLO_Broth_w_o_CV__Difco_["Bacto PPLO Broth w/o CV (Difco)"]
    CultureMech_015909 --> ing0_Bacto_PPLO_Broth_w_o_CV__Difco_
    ing1_Horse_serum["Horse serum"]
    CultureMech_015909 --> ing1_Horse_serum
    ing2_25__Yeast_extract_solution["25% Yeast extract solution"]
    CultureMech_015909 --> ing2_25__Yeast_extract_solution
    ing3_Phenol_red["Phenol red"]
    CultureMech_015909 --> ing3_Phenol_red
    CHEBI_31991["CHEBI:31991"]
    ing3_Phenol_red -.-> CHEBI_31991
    ing4_L_Arginine["L-Arginine"]
    CultureMech_015909 --> ing4_L_Arginine
    CHEBI_16467["CHEBI:16467"]
    ing4_L_Arginine -.-> CHEBI_16467
    ing5_L_Glutamine["L-Glutamine"]
    CultureMech_015909 --> ing5_L_Glutamine
    CHEBI_18050["CHEBI:18050"]
    ing5_L_Glutamine -.-> CHEBI_18050
    ing6_100X_MEM_Vitamins__Gibco_["100X MEM Vitamins (Gibco)"]
    CultureMech_015909 --> ing6_100X_MEM_Vitamins__Gibco_
    ing7_Agar__if_needed_["Agar (if needed)"]
    CultureMech_015909 --> ing7_Agar__if_needed_
    CHEBI_2509["CHEBI:2509"]
    ing7_Agar__if_needed_ -.-> CHEBI_2509
    ing8_Distilled_water["Distilled water"]
    CultureMech_015909 --> ing8_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing8_Distilled_water -.-> CHEBI_15377

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 Bacto PPLO Broth w/o CV (Difco) 14.7 G_PER_L
2 Horse serum 200 ML_PER_L
3 25% Yeast extract solution 100 ML_PER_L
4 Phenol red
C19H14O5S 354.383 g/mol
20 MG_PER_L CHEBI:31991
phenol red
5 L-Arginine
C6H14N4O2 174.204 g/mol
2.1 G_PER_L CHEBI:16467
L-arginine
6 L-Glutamine
C5H10N2O3 146.146 g/mol
1.46 G_PER_L CHEBI:18050
L-glutamine
7 100X MEM Vitamins (Gibco) 1 ML_PER_L
8 Agar (if needed) 15 G_PER_L CHEBI:2509
agar
 NBRC lists this component as optional when a solid medium is needed.
9 Distilled water
H2O 18.015 g/mol
600 ML_PER_L CHEBI:15377
water

Preparation

  1. *Sterilize by filtration, and add to another component sterilized by autoclaving.
  2. **Boil 250 g of dried baker's yeast suspended in 1 L of distilled water for 30 min, remove yeast cells by centrifugation, and sterilize by filtration.
  3. ***Prepare 100 mM solution and sterilize by filtration.
  4. The anaerobic growth on plates was carried out in an anaerobic jar equipped with a CO2-generating gas pack (Mitsubishi Gas Chemical Company Inc., Tokyo, Japan).

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-10-02T05:20:01.508978+00:00 nbrc-live-import Scraped from NBRC catalogue Direct import from NBRC Medium 1407. Ingredients captured verbatim from the live catalogue.
2026-10-02T05:20:01.508978+00:00 culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:015909