Overview
- External ontology
- UTEX:one-to-one-dyiii-pea-gr-plus-medium (source link unavailable) — (1:1) DYIII/PEA + Gr+ Medium
- Applications
-
- Algae cultivation
- Phytoplankton culture
- Microalgae research
- Notes
- UTEX prepares (1:1) DYIII/PEA + Gr+ Medium by autoclaving one organic dried pea with 90 mL DYIII Medium, then adding 90 mL pasteurized Soilwater: GR+ Medium and DAS Vitamin Cocktail.
Composition
flowchart LR
CultureMech_000001["1_1_dyiii_pea_gr_medium"]
ing0_Pea["Pea"]
CultureMech_000001 --> ing0_Pea
Ingredients (1)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Pea | 1 per 200 mL VARIABLE | UTEX lists one organic dried pea per 200 mL. |
Preparation
- Add one organic dried pea to 90 mL of DYIII Medium.
- Autoclave the DYIII Medium and pea mixture.
- Add 90 mL of pasteurized Soilwater: GR+ Medium.
- Add 4 drops of DAS Vitamin Cocktail.
- Store at refrigerator temperature.
Solutions
DYIII Medium
Dose: 500 ML_PER_L
Converted from 90 mL prepared DYIII Medium per 180 mL recipe.
Soilwater: GR+ Medium
Dose: 500 ML_PER_L
Converted from 90 mL pasteurized Soilwater: GR+ Medium per 180 mL recipe.
DAS Vitamin Cocktail
Dose: 4 drops per 180 mL VARIABLE
| Ingredient | Concentration | Notes |
|---|---|---|
| dH2O | 1000 ML_PER_L | UTEX starts the DAS Vitamin Cocktail with approximately 85 mL dH2O and brings the final volume to 100 mL. |
| Tricine | 9 G_PER_L | Converted from 0.9 g Tricine per 100 mL; UTEX adjusts the cocktail to pH 8.0. |
| Thiamine | 0.67 G_PER_L | Converted from 0.067 g/100 mL; UTEX reports 2.0 mM stock and 0.99 uM final in DAS. |
| Biotin | 0.01 G_PER_L | Converted from 0.001 g/100 mL; UTEX reports 0.041 mM stock and 0.02 uM final in DAS. |
| Vitamin B12 | 0.008 G_PER_L | Converted from 0.0008 g/100 mL; UTEX reports adding B12 after autoclaving, 0.0059 mM stock, and 0.0029 uM final in DAS. |
| Pantothenate | 0.02 G_PER_L | Converted from 0.002 g/100 mL; UTEX reports 0.091 mM stock and 0.046 uM final in DAS. |
| PABA | 0.04 G_PER_L | Converted from 0.004 g/100 mL p-aminobenzoic acid; UTEX reports 0.291 mM stock and 0.15 uM final in DAS. |
UTEX lists 4 drops DAS Vitamin Cocktail per 180 mL recipe.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-28T00:00:00+00:00 | utex-import | Imported from UTEX Culture Collection | Source ID: one-to-one-dyiii-pea-gr-plus-medium, URL: https://utex.org/products/one-to-one-dyiii-pea-gr-plus-medium |
| 2026-03-13T06:18:52.793288Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000001 |
| 2026-03-17T19:45:03.774945+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "1:1 DYIII/PEA + Gr+ Medium" to "1_1_dyiii_pea_gr_medium" for consistent matching |
| 2026-05-16T09:02:55.691141+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1; references.reference_id->reference=2; category->lowercase=1 |
| 2026-05-16T09:11:00.897778+00:00 | migrate_preparation_steps.py | MIGRATED_PREPARATION_STEPS | instruction->action+description for 9 step(s) |
| 2026-08-30T00:58:10.356852+00:00 | repair_utex_ingredients.py | REPAIRED_UTEX_COMPOSITION | Restored 4 ingredient name(s) and amount(s) from a re-fetched UTEX capture. The original fetcher read fixed column positions, so the row ordinal was stored as the ingredient name, the component name as the amount, and the Amount column was never read; concentration was then filled in as G_PER_L regardless. Source: https://utex.org/pages/algal-culture-media, captured 2026-08-29T17:46:40.207779. |
| 2026-09-06T00:00:00-07:00 | repair_utex_dasycladales_pea_score35.py | RESOLVED_UTEX_DASYCLADALES_PEA_SCORE35 | UTEX prepares (1:1) DYIII/PEA + Gr+ Medium by autoclaving one organic dried pea with 90 mL DYIII Medium, then adding 90 mL pasteurized Soilwater: GR+ Medium and DAS Vitamin Cocktail. |