1_2_enriched_seawater (CultureMech:000003)

Overview

External ontology

UTEX:one-half-enriched-seawater-medium (source link unavailable) — 1/2 Enriched Seawater Medium

Applications

Algae cultivation
Phytoplankton culture
Microalgae research

Notes

UTEX prepares this dilution by mixing prepared Enriched Seawater Medium with sterile distilled water in a 1:1 ratio for 15 ppt salinity.

Composition

flowchart LR
    CultureMech_000003["1_2_enriched_seawater"]
    ing0_dH2O["dH2O"]
    CultureMech_000003 --> ing0_dH2O
    CHEBI_15377["CHEBI:15377"]
    ing0_dH2O -.-> CHEBI_15377

Ingredients (1)

#	Ingredient	Concentration	Ontology	Notes
1	dH2O H2O 18.015 g/mol	500 ML_PER_L	CHEBI:15377 water	UTEX lists 500 mL sterile dH2O per 1 L final medium.

Preparation

Combine prepared Enriched Seawater Medium with sterile dH2O.
Store at refrigerator temperature.

Solutions

Enriched Seawater Medium

Dose: 500 ML_PER_L

CultureMech:000166

UTEX lists 500 mL prepared Enriched Seawater Medium per 1 L final medium.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-28T00:00:00+00:00	utex-import	Imported from UTEX Culture Collection	Source ID: one-half-enriched-seawater-medium, URL: https://utex.org/products/one-half-enriched-seawater-medium
2026-03-13T06:18:52.804571Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:000003
2026-03-17T19:45:03.784926+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "1/2 Enriched Seawater" to "1_2_enriched_seawater" for consistent matching
2026-05-16T09:02:55.707165+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=1; references.reference_id->reference=2; category->lowercase=1
2026-05-16T09:11:00.908740+00:00	migrate_preparation_steps.py	MIGRATED_PREPARATION_STEPS	instruction->action+description for 9 step(s)
2026-08-30T00:58:30.415953+00:00	repair_utex_ingredients.py	REPAIRED_UTEX_COMPOSITION	Restored 2 ingredient name(s) and amount(s) from a re-fetched UTEX capture. The original fetcher read fixed column positions, so the row ordinal was stored as the ingredient name, the component name as the amount, and the Amount column was never read; concentration was then filled in as G_PER_L regardless. Source: https://utex.org/pages/algal-culture-media, captured 2026-08-29T17:46:40.207779.
2026-09-06T00:00:00-07:00	repair_utex_algal_blends.py	RESOLVED_UTEX_ALGAL_BLEND_GRAPH	UTEX prepares this dilution by mixing prepared Enriched Seawater Medium with sterile distilled water in a 1:1 ratio for 15 ppt salinity.
2026-10-10T01:47:47.012900+00:00	codex	CURATED_UTEX_ES_NESTED_STOCKS_AND_INGREDIENT_EVIDENCE	Added 2 claim-linked evidence entries and added or enriched 1 bibliography entry in this record. Related formulation/stock limitations for this review batch: The parent HTML total-volume heading conflicts with the per-liter-of-seawater procedure/PDF. Expanded recipes retain absolute liquid inputs; no measured final volume or final constituent concentrations are inferred. The enrichment source lists vitamin doses but omits a later vitamin-addition instruction; no missing step is invented. ES Fe formula and Sigma F1513 catalog identity remain unreconciled. Only the source-named ferrous-ammonium-sulfate stock mass is supported; no catalog-based replacement or molar conversion is made. The P-II source permits a cobalt sulfate alternative without an exact replacement mass/hydrate. Only the listed cobalt chloride hexahydrate amount is supported. No exact glycerophosphate-pentahydrate ontology identifier was established. Unsupported sn-glycerol-3-phosphate assertions are removed; the immutable MIM index remains unchanged. Generic lighting, temperature, application and growth claims remain outside this ingredient review; no taxa-media associations are added. The 2/3 PDF copyright notice does not establish a publication year for the live HTML. Supplier pages inspected by the web tool are not full downloaded captures.