1_3_soil_seawater_medium

Overview

External ontology

UTEX:one-third-soil-plus-seawater-medium (source link unavailable) — 1/3 Soil+Seawater Medium

Applications

• Algae cultivation
• Phytoplankton culture
• Microalgae research

Notes

The source uses 67 mL Pasteurized Seawater and 133 mL dH2O per 200 mL liquid-input recipe; 1:2 is approximate shorthand, not exact volume fractions.

Composition

flowchart LR
    CultureMech_000007["1_3_soil_seawater_medium"]
    ing0_Green_House_Soil["Green House Soil"]
    CultureMech_000007 --> ing0_Green_House_Soil
    ing1_Pasteurized_Seawater["Pasteurized Seawater"]
    CultureMech_000007 --> ing1_Pasteurized_Seawater
    ing2_dH2O["dH2O"]
    CultureMech_000007 --> ing2_dH2O
    CHEBI_15377["CHEBI:15377"]
    ing2_dH2O -.-> CHEBI_15377

Ingredients (3)

Preparation

1. Combine Green House Soil and Pasteurized Seawater with dH2O; add calcium carbonate only for the optional supplemented formulation.
2. Cover the medium container and steam for two consecutive days, three hours on each day. Raise temperature gradually to approximately 95 C in 15 minutes, then just over 98 C for the three-hour duration. Cool gradually at room temperature.
3. Refrigerate for at least 24 hours, then bring to room temperature before use.

Target organisms

No target organisms are recorded.

Recipe variants

• Name

  1/3 Soil+Seawater Medium with optional calcium carbonate

  Relationship

  SUPPLEMENTED_VARIANT

  Description

  Source-listed optional calcium carbonate supplement.

  Modifications

  • Optionally add 1 mg calcium carbonate (Fisher C 64) to the source 200 mL recipe before steaming.

  Purpose

  Preserve the explicitly optional carbonate formulation

  Evidence

  • Reference

    https://utex.org/products/one-third-soil-plus-seawater-medium

    Supports

    SUPPORT

    Snippet

    1 mg

    Explanation

    Supports variants[0].modifications and removal of the mandatory carbonate node at old ingredients[1]. Separate source component excerpt: 'CaCO3 (optional) (Fisher C 64)'. Separate printed concentration excerpt: '0.05 mM'; the printed molarity is a source annotation for the optional formulation, not a universal parent concentration. Move optional calcium carbonate out of mandatory parent composition into an evidence-backed inline variant. Preserve the literal 1 mg recipe dose, supplier/identity provenance and printed rounded 0.05 mM as a source annotation; do not assert universal carbonate addition. Calculation: No new concentration inferred; the source-literal amount belongs only to the optional formulation.. Separate basis excerpt: 'For 200 mL Total'. Locator: Formulation table row position 2; Directions total-volume heading.

Provenance