1_4_soil_seawater_medium (CultureMech:000009)

Overview

External ontology

UTEX:one-fourth-soil-plus-seawater-medium (source link unavailable) — 1/4 Soil+Seawater Medium

Applications

Algae cultivation
Phytoplankton culture
Microalgae research

Notes

UTEX prepares 1/4 Soil+Seawater Medium by combining Pasteurized Seawater and dH2O 1:3 before steaming with Green House Soil.

Composition

flowchart LR
    CultureMech_000009["1_4_soil_seawater_medium"]
    ing0_Green_House_Soil["Green House Soil"]
    CultureMech_000009 --> ing0_Green_House_Soil
    ing1_Pasteurized_Seawater["Pasteurized Seawater"]
    CultureMech_000009 --> ing1_Pasteurized_Seawater
    ing2_dH2O["dH2O"]
    CultureMech_000009 --> ing2_dH2O
    CHEBI_15377["CHEBI:15377"]
    ing2_dH2O -.-> CHEBI_15377

Ingredients (3)

#	Ingredient	Concentration	Ontology	Notes
1	Green House Soil			The source fixes the soil dose at 1 tsp for its 200 mL recipe. Teaspoon size and soil density are not established; no mass or standardized volume conversion is asserted.
2	Pasteurized Seawater	250 ML_PER_L (per liter of the combined stated seawater and dH2O input volumes, before soil addition)	ENVO:00002149 sea water	50 mL / 0.2 L combined stated liquid inputs = 250 mL/L of input liquids. This input-volume ratio is not asserted as a measured final-medium concentration.
3	dH2O H2O 18.015 g/mol	750 ML_PER_L (per liter of the combined stated seawater and dH2O input volumes, before soil addition)	CHEBI:15377 water	150 mL / 0.2 L combined stated liquid inputs = 750 mL/L of input liquids. This input-volume ratio is not asserted as a measured final-medium concentration.

Preparation

Combine Green House Soil and Pasteurized Seawater with dH2O; add calcium carbonate only for the optional supplemented formulation.
Cover the medium container and steam for two consecutive days, three hours on each day. Raise temperature gradually to approximately 95 C in 15 minutes, then just over 98 C for the three-hour duration. Cool gradually at room temperature.
Refrigerate for at least 24 hours, then bring to room temperature before use.

Target organisms

No target organisms are recorded.

Recipe variants

Name
1/4 Soil+Seawater Medium with optional calcium carbonate
Relationship
SUPPLEMENTED_VARIANT
Description
Source-listed optional calcium carbonate supplement.
Modifications
- Optionally add 1 mg calcium carbonate (Fisher C 64) to the source 200 mL recipe before steaming.
Purpose
Preserve the explicitly optional carbonate formulation
Evidence
- Reference
  https://utex.org/products/one-fourth-soil-plus-seawater-medium
  Supports
  SUPPORT
  Snippet
  1 mg
  Explanation
  Supports variants[0].modifications and removal of the mandatory carbonate node at old ingredients[1]. Separate source component excerpt: 'CaCO3 (optional) (Fisher C 64)'. Separate printed concentration excerpt: '0.05 mM'; the printed molarity is a source annotation for the optional formulation, not a universal parent concentration. Move optional calcium carbonate out of mandatory parent composition into an evidence-backed inline variant. Preserve the literal 1 mg recipe dose, supplier/identity provenance and printed rounded 0.05 mM as a source annotation; do not assert universal carbonate addition. Calculation: No new concentration inferred; the source-literal amount belongs only to the optional formulation.. Separate basis excerpt: 'For 200 mL Total'. Locator: Formulation table row position 2; Directions total-volume heading.

Provenance

Timestamp	Curator	Action	Notes
2026-01-28T00:00:00+00:00	utex-import	Imported from UTEX Culture Collection	Source ID: one-fourth-soil-plus-seawater-medium, URL: https://utex.org/products/one-fourth-soil-plus-seawater-medium
2026-03-13T06:18:52.832973Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:000009
2026-03-17T19:45:03.811290+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "1/4 Soil + Seawater Medium" to "1_4_soil_seawater_medium" for consistent matching
2026-05-16T09:02:55.740784+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=1; references.reference_id->reference=2; category->lowercase=1
2026-05-16T09:11:00.940804+00:00	migrate_preparation_steps.py	MIGRATED_PREPARATION_STEPS	instruction->action+description for 9 step(s)
2026-08-30T00:58:30.455575+00:00	repair_utex_ingredients.py	REPAIRED_UTEX_COMPOSITION	Restored 4 ingredient name(s) and amount(s) from a re-fetched UTEX capture. The original fetcher read fixed column positions, so the row ordinal was stored as the ingredient name, the component name as the amount, and the Amount column was never read; concentration was then filled in as G_PER_L regardless. Source: https://utex.org/pages/algal-culture-media, captured 2026-08-29T17:46:40.207779.
2026-09-06T00:00:00-07:00	repair_utex_chev_soil_variants_score35.py	RESOLVED_UTEX_CHEV_SOIL_SCORE35	UTEX prepares 1/4 Soil+Seawater Medium by combining Pasteurized Seawater and dH2O 1:3 before steaming with Green House Soil.
2026-10-10T08:24:09.034201+00:00	codex	CURATED_UTEX_SOIL_SEAWATER_INPUT_AMOUNTS_AND_OPTIONAL_CARBONATE	Source-backed input amounts, optional carbonate and preparation details. No taxa-media association added; no fixed teaspoon conversion or inferred final volume.