1_5_chev_diatom_medium

CultureMech:000010 algae COMPLEX LIQUID
Original name: 1/5 CHEV Diatom Medium

Overview

External ontology
UTEX:one-fifth-chev-diatom-medium (source link unavailable) — 1/5 CHEV Diatom Medium
Applications
  • Algae cultivation
  • Phytoplankton culture
  • Microalgae research
Notes
UTEX prepares 1/5 CHEV Diatom Medium tube-by-tube from 1 cc CR1 Soil, 0.5 mg/12 mL MgCO3, 12 mL dH2O, and 4 drops Pasteurized Seawater.

Composition

flowchart LR
    CultureMech_000010["1_5_chev_diatom_medium"]
    ing0_CR1_Soil["CR1 Soil"]
    CultureMech_000010 --> ing0_CR1_Soil
    ing1_MgCO3["MgCO3"]
    CultureMech_000010 --> ing1_MgCO3
    CHEBI_31793["CHEBI:31793"]
    ing1_MgCO3 -.-> CHEBI_31793
    ing2_dH2O["dH2O"]
    CultureMech_000010 --> ing2_dH2O
    CHEBI_15377["CHEBI:15377"]
    ing2_dH2O -.-> CHEBI_15377
    ing3_Pasteurized_Seawater["Pasteurized Seawater"]
    CultureMech_000010 --> ing3_Pasteurized_Seawater

Ingredients (4)

# Ingredient Concentration Ontology Notes
1 CR1 Soil 0.001 L Source batch input: 1 cc CR1 Soil per tube in a 15 mL vessel. Stored as 0.001 L of input, not a concentration or final-volume claim.
2 MgCO3
CO3.Mg 84.313 g/mol
MCIB CB486
0.5 MILLIMOLAR CHEBI:31793
magnesium carbonate
 UTEX lists 0.5 mg/12 mL in its Amount column and 0.5 mM in its Final Concentration column. Molarity is transcribed directly, not calculated from the mass input.
3 dH2O
H2O 18.015 g/mol
0.012 L CHEBI:15377
water
 Source batch input: 12 mL dH2O per tube in a 15 mL vessel. Stored as 0.012 L of input, not a concentration or final-volume claim.
4 Pasteurized Seawater 4 drops per 15 mL tube VARIABLE ENVO:00002149
sea water
 Source batch input: 4 drops Pasteurized Seawater per tube in a 15 mL vessel. This is a fixed drop count, not a quantified volume or concentration. The legacy VARIABLE encoding remains unresolved.

Preparation

  1. Add CR1 Soil, MgCO3, dH2O, and Pasteurized Seawater in order to 15 mL glass tubes.
  2. Process the soil and liquid mixture within 6 hours; autoclave for 15 minutes for routine maintenance.
  3. Allow tubes to gas-equilibrate for 2 days before use.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-28T00:00:00+00:00 utex-import Imported from UTEX Culture Collection Source ID: one-fifth-chev-diatom-medium, URL: https://utex.org/products/one-fifth-chev-diatom-medium
2026-03-13T06:18:52.837858Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000010
2026-03-17T19:45:03.815830+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "1/5 CHEV Diatom Medium" to "1_5_chev_diatom_medium" for consistent matching
2026-05-16T09:02:55.745286+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1; references.reference_id->reference=2; category->lowercase=1
2026-05-16T09:11:00.946323+00:00 migrate_preparation_steps.py MIGRATED_PREPARATION_STEPS instruction->action+description for 9 step(s)
2026-08-30T00:58:30.462214+00:00 repair_utex_ingredients.py REPAIRED_UTEX_COMPOSITION Restored 4 ingredient name(s) and amount(s) from a re-fetched UTEX capture. The original fetcher read fixed column positions, so the row ordinal was stored as the ingredient name, the component name as the amount, and the Amount column was never read; concentration was then filled in as G_PER_L regardless. Source: https://utex.org/pages/algal-culture-media, captured 2026-08-29T17:46:40.207779.
2026-09-06T00:00:00-07:00 repair_utex_chev_soil_variants_score35.py RESOLVED_UTEX_CHEV_SOIL_SCORE35 UTEX prepares 1/5 CHEV Diatom Medium tube-by-tube from 1 cc CR1 Soil, 0.5 mg/12 mL MgCO3, 12 mL dH2O, and 4 drops Pasteurized Seawater.
2026-10-09T23:38:36.585055+00:00 codex CURATED_UTEX_CHEV_BATCH_INPUTS_AND_SCOPED_EVIDENCE L quantities are absolute input volumes per single-tube preparation, not final concentrations. Vessel capacity and summed liquid inputs do not establish final volume. The three fixed drop counts retain unresolved legacy VARIABLE encodings; their new evidence supports notes only. No drop-volume estimate is introduced. Modified CHEV's 1 mg/12 mL versus 0.5 mM source discrepancy remains unresolved. Its new evidence supports the discrepancy note, not the retained molarity. Referenced F/2 composition, chemical mappings, preparation, growth conditions and relationships are preserved, not newly certified. No new taxa-media associations.