1_f_2_medium

CultureMech:000012 algae COMPLEX LIQUID
Original name: 1% F/2 Medium

Overview

External ontology
UTEX:1-percent-f-2-medium (source link unavailable) — 1% F/2 Medium
Applications
  • Algae cultivation
  • Phytoplankton culture
  • Microalgae research
Notes
UTEX prepares 1% F/2 Medium from 285 mL non-pasteurized seawater, 600 mL dH2O, the F/2 nutrient and vitamin stocks, and Pasteurized Seawater to bring the recipe to 1 L.

Composition

flowchart LR
    CultureMech_000012["1_f_2_medium"]
    ing0_Seawater_non_sterilized_["Seawater(non-sterilized)"]
    CultureMech_000012 --> ing0_Seawater_non_sterilized_
    ing1_dH2O["dH2O"]
    CultureMech_000012 --> ing1_dH2O
    CHEBI_15377["CHEBI:15377"]
    ing1_dH2O -.-> CHEBI_15377
    ing2_Pasteurized_Seawater["Pasteurized Seawater"]
    CultureMech_000012 --> ing2_Pasteurized_Seawater
    ing3_NaNO3["NaNO3"]
    CultureMech_000012 --> ing3_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing3_NaNO3 -.-> CHEBI_63005
    ing4_Sodium_dihydrogenphosphate_monohydrate["Sodium dihydrogenphosphate monohydrate"]
    CultureMech_000012 --> ing4_Sodium_dihydrogenphosphate_monohydrate
    CHEBI_114249["CHEBI:114249"]
    ing4_Sodium_dihydrogenphosphate_monohydrate -.-> CHEBI_114249
    ing5_Na2SiO3_9H2O["Na2SiO3•9H2O"]
    CultureMech_000012 --> ing5_Na2SiO3_9H2O
    CHEBI_132108["CHEBI:132108"]
    ing5_Na2SiO3_9H2O -.-> CHEBI_132108

Ingredients (6)

# Ingredient Concentration Ontology Notes
1 Seawater(non-sterilized) 285 ML_PER_L ENVO:00002149
sea water
 UTEX lists 285 mL non-pasteurized seawater per 1 L final medium.
2 dH2O
H2O 18.015 g/mol
600 ML_PER_L CHEBI:15377
water
 UTEX lists 600 mL dH2O per 1 L final medium.
3 Pasteurized Seawater ENVO:00002149
sea water
 Use Pasteurized Seawater as needed to bring the recipe to 1 L total after the stated initial liquid inputs. No measured makeup amount or concentration is supplied.
4 NaNO3
NO3.Na 84.994 g/mol
Fisher BP360-500
880 MICROMOLAR CHEBI:63005
sodium nitrate
 UTEX adds 1 mL per liter of a 7.5 g/100 mL dH2O stock.
5 Sodium dihydrogenphosphate monohydrate
H2O.H2O4P.Na 137.991 g/mol
MCIB 742
36 MICROMOLAR CHEBI:114249
sodium dihydrogenphosphate monohydrate
 UTEX adds 1 mL per liter of a 0.5 g/100 mL dH2O stock.
6 Na2SiO3•9H2O
9H2O.2Na.O3Si 284.198 g/mol
Sigma 307815
70 MICROMOLAR CHEBI:132108
sodium silicate nonahydrate
 UTEX adds 1 mL per liter of a 2 g/100 mL dH2O stock, for 70 uM final.

Preparation

  1. Combine 285 mL non-pasteurized seawater and 600 mL dH2O.
  2. Add the F/2 nutrient stocks and Trace Metals Solution while stirring.
  3. Bring the total volume to 1 L with Pasteurized Seawater.
  4. Cover and autoclave the medium.
  5. Add sterile vitamin stocks after the medium cools.
  6. Store at refrigerator temperature.

Solutions

Trace Metals Solution

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
dH2O Water is used to bring Trace Metals Solution to 1 L total; its input amount is not separately quantified.
ZnSO4 x 7 H2O 0.023 G_PER_L Converted from 23 mg/L in the UTEX Trace Metals Solution recipe. Evidence supports the stock mass column only; the source molarity column is discrepant and is not used to infer a corrected molarity or receiving-medium concentration.
MnSO4 x H2O 0.152 G_PER_L Converted from 152 mg/L in the UTEX Trace Metals Solution recipe. Evidence supports the stock mass column only; the source molarity column is discrepant and is not used to infer a corrected molarity or receiving-medium concentration.
Na2MoO4 x 2 H2O 0.0073 G_PER_L Converted from 7.3 mg/L in the UTEX Trace Metals Solution recipe. Evidence supports the stock mass column only; the source molarity column is discrepant and is not used to infer a corrected molarity or receiving-medium concentration.
CoSO4 x 7 H2O 0.014 G_PER_L Converted from 14 mg/L in the UTEX Trace Metals Solution recipe. Evidence supports the stock mass column only; the source molarity column is discrepant and is not used to infer a corrected molarity or receiving-medium concentration.
CuCl2 x 2 H2O 0.0068 G_PER_L Converted from 6.8 mg/L in the UTEX Trace Metals Solution recipe. Evidence supports the stock mass column only; the source molarity column is discrepant and is not used to infer a corrected molarity or receiving-medium concentration.
Fe(NH4)2(SO4)2 x 6 H2O 4.6 G_PER_L UTEX lists 4.6 g/L in the Trace Metals Solution recipe. Evidence supports the stock mass column only; the source molarity column is discrepant and is not used to infer a corrected molarity or receiving-medium concentration.
Na2EDTA x 2 H2O 4.4 G_PER_L UTEX lists 4.4 g/L in the Trace Metals Solution recipe. Evidence supports the stock mass column only; the source molarity column is discrepant and is not used to infer a corrected molarity or receiving-medium concentration.
UTEX adds 1 mL of this prepared stock per liter of 1% F/2 Medium; composition values describe the stock, not final-medium concentrations. The linked stock table has an unresolved mass/molarity discrepancy; its mass column is preserved and no molarity is inferred.

Vitamin B12

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
dH2O The source prepares 200 mL HEPES buffer before adding the vitamin. It does not separately quantify pure-water input; no fixed water concentration is asserted.
HEPES buffer pH 7.8 12 G_PER_L Converted from 2.4 g/200 mL in the UTEX Vitamin B12 stock.
Cyanocobalamin 0.135 G_PER_L Converted from 0.027 g/200 mL in the UTEX Vitamin B12 stock.
UTEX adds 1 mL of this prepared stock per liter of 1% F/2 Medium; composition values describe the stock, not final-medium concentrations.

Biotin Vitamin Solution

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
dH2O The source prepares 200 mL HEPES buffer before adding the vitamin. It does not separately quantify pure-water input; no fixed water concentration is asserted.
HEPES buffer pH 7.8 12 G_PER_L Converted from 2.4 g/200 mL in the UTEX biotin stock.
Biotin 0.025 G_PER_L Converted from 0.005 g/200 mL in the UTEX biotin stock.
UTEX adds 1 mL of this prepared stock per liter of 1% F/2 Medium; composition values describe the stock, not final-medium concentrations.

Thiamine Vitamin Solution

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
dH2O The source prepares 200 mL HEPES buffer before adding the vitamin. It does not separately quantify pure-water input; no fixed water concentration is asserted.
HEPES buffer pH 7.8 12 G_PER_L Converted from 2.4 g/200 mL in the UTEX thiamine stock.
Thiamine hydrochloride 0.335 G_PER_L Converted from 0.067 g/200 mL in the UTEX thiamine stock.
UTEX adds 1 mL of this prepared stock per liter of 1% F/2 Medium; composition values describe the stock, not final-medium concentrations.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-28T00:00:00+00:00 utex-import Imported from UTEX Culture Collection Source ID: 1-percent-f-2-medium, URL: https://utex.org/products/1-percent-f-2-medium
2026-03-13T06:18:52.848558Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000012
2026-03-17T19:45:03.825599+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "1% F/2 Medium" to "1_f_2_medium" for consistent matching
2026-05-16T09:02:55.755744+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1; references.reference_id->reference=2; category->lowercase=1
2026-05-16T09:11:00.957894+00:00 migrate_preparation_steps.py MIGRATED_PREPARATION_STEPS instruction->action+description for 9 step(s)
2026-08-30T00:58:30.476528+00:00 repair_utex_ingredients.py REPAIRED_UTEX_COMPOSITION Restored 9 ingredient name(s) and amount(s) from a re-fetched UTEX capture. The original fetcher read fixed column positions, so the row ordinal was stored as the ingredient name, the component name as the amount, and the Amount column was never read; concentration was then filled in as G_PER_L regardless. Source: https://utex.org/pages/algal-culture-media, captured 2026-08-29T17:46:40.207779.
2026-09-06T00:00:00-07:00 repair_utex_f2_score35.py RESOLVED_UTEX_F2_SCORE35 UTEX prepares 1% F/2 Medium from 285 mL non-pasteurized seawater, 600 mL dH2O, the F/2 nutrient and vitamin stocks, and Pasteurized Seawater to bring the recipe to 1 L.
2026-10-10T00:39:00.252028+00:00 codex CURATED_UTEX_F2_INGREDIENT_AND_STOCK_STAGE_EVIDENCE Trace-metal source mass/molarity discrepancy remains unresolved; only mass-column stock amounts are supported. The parent and 1% HTML/PDF seawater salinity versions differ; neither is silently substituted for the other. The 5% source lists vitamin doses but omits a later vitamin-addition step; no missing procedure is invented. Narrative literature variants and existing organism/growth claims are unchanged and not certified by UTEX sources. No numeric amount is invented for makeup/carrier water, seawater or the NH4MgPO4 pinch.