2x_erdschreibers_medium

CultureMech:000015 algae COMPLEX LIQUID
Original name: 2X Erdschreiber's Medium

Overview

External ontology
UTEX:2x-erdschreibers-medium (source link unavailable) — 2X Erdschreiber's Medium
Applications
  • Algae cultivation
  • Phytoplankton culture
  • Microalgae research
Notes
UTEX prepares 2X Erdschreiber's Medium by substituting pasteurized Supplemented Seawater at 60 ppt for the 30 ppt pasteurized seawater base in Erdschreiber's Medium.

Composition

flowchart LR
    CultureMech_000015["2x_erdschreibers_medium"]
    ing0_Supplemented_Seawater["Supplemented Seawater"]
    CultureMech_000015 --> ing0_Supplemented_Seawater
    ing1_NaNO3["NaNO3"]
    CultureMech_000015 --> ing1_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing1_NaNO3 -.-> CHEBI_63005
    ing2_Na2HPO4_x_7_H2O["Na2HPO4 x 7 H2O"]
    CultureMech_000015 --> ing2_Na2HPO4_x_7_H2O
    CHEBI_34683["CHEBI:34683"]
    ing2_Na2HPO4_x_7_H2O -.-> CHEBI_34683

Ingredients (3)

# Ingredient Concentration Ontology Notes
1 Supplemented Seawater 1000 ML_PER_L UTEX starts the 3 L recipe with pasteurized Supplemented Seawater at 60 ppt.
2 NaNO3
NO3.Na 84.994 g/mol
Fisher BP360-500
2.3 MILLIMOLAR CHEBI:63005
sodium nitrate
 UTEX adds 10 mL of 0.7 M NaNO3 stock per 3 L medium.
3 Na2HPO4 x 7 H2O
HO4P.2Na 141.958 g/mol
Sigma S-9390
0.067 MILLIMOLAR CHEBI:34683
disodium hydrogen phosphate heptahydrate
 UTEX adds 10 mL of 0.02 M Na2HPO4 x 7 H2O stock per 3 L medium.

Preparation

  1. Aseptically add sterile nitrate, phosphate, P-IV Metal Solution, Soilwater: GR+ Medium, and Vitamin B12 stocks to 3 L of pasteurized Supplemented Seawater at 60 ppt.
  2. Swirl to mix and store at refrigerator temperature.

Solutions

P-IV Metal Solution

Dose: 12 ML_PER_L

Stock composition
IngredientConcentrationNotes
dH2O 1000 ML_PER_L UTEX brings P-IV Metal Solution to 1 L with dH2O.
Na2EDTA x 2 H2O 0.75 G_PER_L UTEX lists 0.75 g/L Na2EDTA x 2 H2O, 2 mM final, and dissolves it fully before the other P-IV components.
FeCl3 x 6 H2O 0.097 G_PER_L UTEX lists 0.097 g/L FeCl3 x 6 H2O, for 0.36 mM final.
MnCl2 x 4 H2O 0.041 G_PER_L UTEX lists 0.041 g/L MnCl2 x 4 H2O, for 0.21 mM final.
ZnCl2 0.005 G_PER_L UTEX lists 0.005 g/L ZnCl2, for 0.037 mM final.
CoCl2 x 6 H2O 0.002 G_PER_L UTEX lists 0.002 g/L CoCl2 x 6 H2O, for 0.0084 mM final.
Na2MoO4 x 2 H2O 0.004 G_PER_L UTEX lists 0.004 g/L Na2MoO4 x 2 H2O, for 0.017 mM final.
UTEX adds 12 mL P-IV Metal Solution per liter.

Soilwater: GR+ Medium

Dose: 50 ML_PER_L

CultureMech:000225

UTEX lists 50 mL prepared Soilwater: GR+ Medium per liter.

Vitamin B12

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
dH2O 1000 ML_PER_L UTEX prepares the Vitamin B12 stock in 200 mL HEPES buffer.
HEPES buffer pH 7.8 12 G_PER_L Converted from 2.4 g/200 mL in the UTEX Vitamin B12 stock.
Vitamin B12 0.135 G_PER_L Converted from 0.027 g/200 mL in the UTEX Vitamin B12 stock.
UTEX adds 1 mL Vitamin B12 stock per liter.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-28T00:00:00+00:00 utex-import Imported from UTEX Culture Collection Source ID: 2x-erdschreibers-medium, URL: https://utex.org/products/2x-erdschreibers-medium
2026-03-13T06:18:52.863844Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000015
2026-03-17T19:45:03.839215+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "2X Erdschreiber's Medium" to "2x_erdschreibers_medium" for consistent matching
2026-05-16T09:02:55.788492+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1; references.reference_id->reference=2; category->lowercase=1
2026-05-16T09:11:00.994580+00:00 migrate_preparation_steps.py MIGRATED_PREPARATION_STEPS instruction->action+description for 9 step(s)
2026-08-30T00:58:30.527184+00:00 repair_utex_ingredients.py REPAIRED_UTEX_COMPOSITION Restored 6 ingredient name(s) and amount(s) from a re-fetched UTEX capture. The original fetcher read fixed column positions, so the row ordinal was stored as the ingredient name, the component name as the amount, and the Amount column was never read; concentration was then filled in as G_PER_L regardless. Source: https://utex.org/pages/algal-culture-media, captured 2026-08-29T17:46:40.207779.
2026-09-06T00:00:00-07:00 repair_utex_bristol_bold_score35.py RESOLVED_UTEX_BRISTOL_BOLD_SCORE35 UTEX prepares 2X Erdschreiber's Medium by substituting pasteurized Supplemented Seawater at 60 ppt for the 30 ppt pasteurized seawater base in Erdschreiber's Medium.