2x_soil_seawater_medium (CultureMech:000016)

Overview

External ontology

UTEX:2x-soil-plus-seawater-medium (source link unavailable) — 2X Soil+Seawater Medium

Applications

Algae cultivation
Phytoplankton culture
Microalgae research

Notes

UTEX prepares 2X Soil+Seawater Medium for 60 ppt marine cultures by combining Green House Soil, CaCO3, and Supplemented Seawater before steam pasteurization.

Composition

flowchart LR
    CultureMech_000016["2x_soil_seawater_medium"]
    ing0_Green_House_Soil["Green House Soil"]
    CultureMech_000016 --> ing0_Green_House_Soil
    ing1_CaCO3["CaCO3"]
    CultureMech_000016 --> ing1_CaCO3
    CHEBI_3311["CHEBI:3311"]
    ing1_CaCO3 -.-> CHEBI_3311

Ingredients (2)

#	Ingredient	Concentration	Ontology	Notes
1	Green House Soil	25 ML_PER_L (per liter of Supplemented Seawater before soil addition)		5 mL soil / 0.2 L Supplemented Seawater = 25 mL per liter of carrier. This input-volume ratio is not asserted as a measured final-medium concentration.
2	CaCO3 CO3.Ca 100.086 g/mol Fisher C 64	0.05 MILLIMOLAR	CHEBI:3311 calcium carbonate	The source gives 1 mg calcium carbonate per 200 mL Supplemented Seawater and prints 0.05 mM in its final-concentration column. The rounded molarity is retained as a source assertion, not an independently calculated final concentration.

Preparation

Combine Green House Soil, CaCO3, and Supplemented Seawater.
Cover the medium container and steam for two consecutive days, three hours on each day. Raise temperature gradually to approximately 95 C in 15 minutes, then just over 98 C for the three-hour duration. Cool gradually at room temperature.
Refrigerate for at least 24 hours, then bring to room temperature before use.

Solutions

Supplemented Seawater

Stock composition
Ingredient	Concentration	Notes
Pasteurized Seawater		Start with approximately 900 mL Pasteurized Seawater; add the stock components, then bring total volume to 1 L with Pasteurized Seawater. The exact combined starting and makeup seawater volume is unspecified.
NaCl	23 G_PER_L	UTEX lists 23 g/L NaCl, for 393.8 mM final in the stock.
MgCl2 x 6 H2O	5 G_PER_L	UTEX lists 5 g/L MgCl2 x 6 H2O, for 24.6 mM final in the stock.
Na2SO4	4 G_PER_L	UTEX lists 4 g/L Na2SO4, for 28.2 mM final in the stock.

Use 200 mL Supplemented Seawater before adding 5 mL soil and 1 mg calcium carbonate. No measured final-medium volume is asserted.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-28T00:00:00+00:00	utex-import	Imported from UTEX Culture Collection	Source ID: 2x-soil-plus-seawater-medium, URL: https://utex.org/products/2x-soil-plus-seawater-medium
2026-03-13T06:18:52.868764Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:000016
2026-03-17T19:45:03.843656+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "2X Soil + Seawater Medium" to "2x_soil_seawater_medium" for consistent matching
2026-05-16T09:02:55.793217+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=1; references.reference_id->reference=2; category->lowercase=1
2026-05-16T09:11:01.000156+00:00	migrate_preparation_steps.py	MIGRATED_PREPARATION_STEPS	instruction->action+description for 9 step(s)
2026-08-30T00:58:30.534434+00:00	repair_utex_ingredients.py	REPAIRED_UTEX_COMPOSITION	Restored 3 ingredient name(s) and amount(s) from a re-fetched UTEX capture. The original fetcher read fixed column positions, so the row ordinal was stored as the ingredient name, the component name as the amount, and the Amount column was never read; concentration was then filled in as G_PER_L regardless. Source: https://utex.org/pages/algal-culture-media, captured 2026-08-29T17:46:40.207779.
2026-09-06T00:00:00-07:00	repair_utex_2x_soil_seawater_score35.py	RESOLVED_UTEX_2X_SOIL_SEAWATER_SCORE35	UTEX prepares 2X Soil+Seawater Medium for 60 ppt marine cultures by combining Green House Soil, CaCO3, and Supplemented Seawater before steam pasteurization.
2026-10-10T08:24:09.034201+00:00	codex	CURATED_UTEX_SOIL_SEAWATER_INPUT_AMOUNTS_AND_OPTIONAL_CARBONATE	Source-backed input amounts, optional carbonate and preparation details. No taxa-media association added; no fixed teaspoon conversion or inferred final volume.