5_3_soil_seawater_agar_medium

CultureMech:000019 algae COMPLEX SOLID_AGAR
Original name: 5/3 Soil + Seawater Agar Medium

Overview

External ontology
UTEX:five-thirds-soil-plus-seawater-agar-medium (source link unavailable) — 5/3 Soil+Seawater Agar Medium
Applications
  • Algae cultivation
  • Phytoplankton culture
  • Microalgae research
Notes
UTEX prepares this 48 ppt agar variant by filtering prepared Soil+Seawater Medium, adding 15 g/L natural sea-salt, and solidifying with 15 g/L agar.

Composition

flowchart LR
    CultureMech_000019["5_3_soil_seawater_agar_medium"]
    ing0_Natural_sea_salt["Natural sea-salt"]
    CultureMech_000019 --> ing0_Natural_sea_salt
    ing1_Agar["Agar"]
    CultureMech_000019 --> ing1_Agar
    CHEBI_2509["CHEBI:2509"]
    ing1_Agar -.-> CHEBI_2509

Ingredients (2)

# Ingredient Concentration Ontology Notes
1 Natural sea-salt 15 G_PER_L UTEX lists 15 g/L natural sea-salt; retained as an ungrounded mixture.
2 Agar 15 G_PER_L (per liter of prepared Soil+Seawater Medium before salt and agar additions) CHEBI:2509
agar
 15 g agar / 1 L prepared input medium = 15 g per liter of input medium, not a newly measured final-volume concentration. This input-volume ratio is not asserted as a measured final-medium concentration.

Preparation

  1. Filter 1 L of Soil+Seawater Medium to remove soil particles.
  2. Add 15 g natural sea-salt to the filtered 1 L prepared medium and wait until fully dissolved.
  3. Add 15 g agar to the flask; do not mix. Cover and autoclave the medium.
  4. Store at refrigerator temperature.

Solutions

Soil+Seawater Medium

CultureMech:000221

Filter 1 L prepared Soil+Seawater Medium before adding sea-salt and agar. The source does not measure a final carrier fraction after those additions.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-28T00:00:00+00:00 utex-import Imported from UTEX Culture Collection Source ID: five-thirds-soil-plus-seawater-agar-medium, URL: https://utex.org/products/five-thirds-soil-plus-seawater-agar-medium
2026-03-13T06:18:52.882354Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000019
2026-03-17T19:45:03.855967+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "5/3 Soil + Seawater Agar Medium" to "5_3_soil_seawater_agar_medium" for consistent matching
2026-05-16T09:02:55.805451+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1; references.reference_id->reference=2; category->lowercase=1
2026-05-16T09:11:01.009907+00:00 migrate_preparation_steps.py MIGRATED_PREPARATION_STEPS instruction->action+description for 9 step(s)
2026-08-30T00:58:30.547165+00:00 repair_utex_ingredients.py REPAIRED_UTEX_COMPOSITION Restored 2 ingredient name(s) and amount(s) from a re-fetched UTEX capture. The original fetcher read fixed column positions, so the row ordinal was stored as the ingredient name, the component name as the amount, and the Amount column was never read; concentration was then filled in as G_PER_L regardless. Source: https://utex.org/pages/algal-culture-media, captured 2026-08-29T17:46:40.207779.
2026-09-06T00:00:00-07:00 repair_utex_algal_blends.py RESOLVED_UTEX_ALGAL_BLEND_GRAPH UTEX prepares this 48 ppt agar variant by filtering prepared Soil+Seawater Medium, adding 15 g/L natural sea-salt, and solidifying with 15 g/L agar.
2026-10-10T08:24:09.034201+00:00 codex CURATED_UTEX_SOIL_SEAWATER_INPUT_AMOUNTS_AND_OPTIONAL_CARBONATE Source-backed input amounts, optional carbonate and preparation details. No taxa-media association added; no fixed teaspoon conversion or inferred final volume.