allen_medium

CultureMech:000024 algae COMPLEX LIQUID
Original name: Allen Medium

Overview

External ontology
UTEX:allen-medium (source link unavailable) — Allen Medium
Applications
  • Algae cultivation
  • Phytoplankton culture
  • Microalgae research
pH
7.8
Notes
UTEX prepares Allen Medium from dH2O with HEPES buffer, nitrate, P-IV Metal Solution, phosphate, magnesium, carbonate, calcium, silicate, and citric acid stocks adjusted to pH 7.8.

Composition

flowchart LR
    CultureMech_000024["allen_medium"]
    ing0_dH2O["dH2O"]
    CultureMech_000024 --> ing0_dH2O
    CHEBI_15377["CHEBI:15377"]
    ing0_dH2O -.-> CHEBI_15377
    ing1_HEPES_buffer["HEPES buffer"]
    CultureMech_000024 --> ing1_HEPES_buffer
    CHEBI_42334["CHEBI:42334"]
    ing1_HEPES_buffer -.-> CHEBI_42334
    ing2_NaNO3["NaNO3"]
    CultureMech_000024 --> ing2_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing2_NaNO3 -.-> CHEBI_63005
    ing3_K2HPO4["K2HPO4"]
    CultureMech_000024 --> ing3_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4 -.-> CHEBI_131527
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000024 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_Na2CO3["Na2CO3"]
    CultureMech_000024 --> ing5_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing5_Na2CO3 -.-> CHEBI_29377
    ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000024 --> ing6_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing7_Na2SiO3_x_9_H2O["Na2SiO3 x 9 H2O"]
    CultureMech_000024 --> ing7_Na2SiO3_x_9_H2O
    CHEBI_132108["CHEBI:132108"]
    ing7_Na2SiO3_x_9_H2O -.-> CHEBI_132108
    ing8_Citric_Acid_x_H2O["Citric Acid x H2O"]
    CultureMech_000024 --> ing8_Citric_Acid_x_H2O
    CHEBI_30769["CHEBI:30769"]
    ing8_Citric_Acid_x_H2O -.-> CHEBI_30769

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 dH2O
H2O 18.015 g/mol
1000 ML_PER_L CHEBI:15377
water
 UTEX starts the 1 L Allen Medium recipe with approximately 950 mL dH2O.
2 HEPES buffer
C8H18N2O4S 238.309 g/mol
Sigma H-3375
0.01 MOLAR CHEBI:42334
HEPES
 UTEX lists 2.3 g/L HEPES buffer, for 0.01 M final.
3 NaNO3
NO3.Na 84.994 g/mol
Fisher BP360-500
0.018 MOLAR CHEBI:63005
sodium nitrate
 UTEX lists 1.5 g/L NaNO3, for 0.018 M final.
4 K2HPO4
HO4P.2K 174.174 g/mol
Sigma P 3786
0.215 MILLIMOLAR CHEBI:131527
dipotassium hydrogen phosphate
 UTEX adds 5 mL/L of a 1.5 g/200 mL dH2O stock.
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
Sigma 230391
0.152 MILLIMOLAR CHEBI:31795
magnesium sulfate heptahydrate
 UTEX adds 5 mL/L of a 1.5 g/200 mL dH2O stock.
6 Na2CO3
CO3.2Na 105.988 g/mol
Baker 3604
0.19 MILLIMOLAR CHEBI:29377
sodium carbonate
 UTEX adds 5 mL/L of a 0.8 g/200 mL dH2O stock.
7 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
Sigma C-3881
0.17 MILLIMOLAR CHEBI:86158
calcium chloride dihydrate
 UTEX adds 10 mL/L of a 0.5 g/200 mL dH2O stock.
8 Na2SiO3 x 9 H2O
9H2O.2Na.O3Si 284.198 g/mol
Sigma 307815
0.204 MILLIMOLAR CHEBI:132108
sodium silicate nonahydrate
 UTEX adds 10 mL/L of a 1.16 g/200 mL dH2O stock.
9 Citric Acid x H2O
C6H8O7 192.123 g/mol
Fisher A 104
0.029 MILLIMOLAR CHEBI:30769
citric acid
 UTEX adds 1 mL/L of a 1.2 g/200 mL dH2O stock.

Preparation

  1. To approximately 950 mL dH2O, add HEPES buffer, NaNO3, P-IV Metal Solution, and the listed mineral stocks.
  2. Adjust the medium to pH 7.8.
  3. Bring the total volume to 1 L with dH2O.
  4. Cover and autoclave the medium.
  5. Store at refrigerator temperature.

Solutions

P-IV Metal Solution

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
dH2O 1000 ML_PER_L UTEX brings P-IV Metal Solution to 1 L with dH2O.
Na2EDTA x 2 H2O 0.75 G_PER_L UTEX lists 0.75 g/L Na2EDTA x 2 H2O, 2 mM final, and dissolves it fully before the other P-IV components.
FeCl3 x 6 H2O 0.097 G_PER_L UTEX lists 0.097 g/L FeCl3 x 6 H2O, for 0.36 mM final.
MnCl2 x 4 H2O 0.041 G_PER_L UTEX lists 0.041 g/L MnCl2 x 4 H2O, for 0.21 mM final.
ZnCl2 0.005 G_PER_L UTEX lists 0.005 g/L ZnCl2, for 0.037 mM final.
CoCl2 x 6 H2O 0.002 G_PER_L UTEX lists 0.002 g/L CoCl2 x 6 H2O, for 0.0084 mM final.
Na2MoO4 x 2 H2O 0.004 G_PER_L UTEX lists 0.004 g/L Na2MoO4 x 2 H2O, for 0.017 mM final.
UTEX adds 1 mL P-IV Metal Solution per liter.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-28T00:00:00+00:00 utex-import Imported from UTEX Culture Collection Source ID: allen-medium, URL: https://utex.org/products/allen-medium
2026-03-13T06:18:52.912674Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000024
2026-03-17T19:45:03.882079+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Allen Medium" to "allen_medium" for consistent matching
2026-05-16T09:02:55.826760+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1; references.reference_id->reference=2; category->lowercase=1
2026-05-16T09:11:01.034179+00:00 migrate_preparation_steps.py MIGRATED_PREPARATION_STEPS instruction->action+description for 9 step(s)
2026-08-30T00:58:30.578348+00:00 repair_utex_ingredients.py REPAIRED_UTEX_COMPOSITION Restored 9 ingredient name(s) and amount(s) from a re-fetched UTEX capture. The original fetcher read fixed column positions, so the row ordinal was stored as the ingredient name, the component name as the amount, and the Amount column was never read; concentration was then filled in as G_PER_L regardless. Source: https://utex.org/pages/algal-culture-media, captured 2026-08-29T17:46:40.207779.
2026-09-06T00:00:00-07:00 repair_utex_bristol_bold_score35.py RESOLVED_UTEX_BRISTOL_BOLD_SCORE35 UTEX prepares Allen Medium from dH2O with HEPES buffer, nitrate, P-IV Metal Solution, phosphate, magnesium, carbonate, calcium, silicate, and citric acid stocks adjusted to pH 7.8.