bg_11_n_medium

CultureMech:000028 algae COMPLEX LIQUID
Original name: BG-11(-N) Medium

Overview

External ontology
UTEX:bg-11-n-medium (source link unavailable) — BG-11(-N) Medium
Applications
  • Algae cultivation
  • Phytoplankton culture
  • Microalgae research
Notes
UTEX prepares liquid BG-11(-N) Medium from seven mineral stocks and BG-11 Trace Metals Solution diluted in dH2O before autoclaving; this nitrogen-free BG-11 recipe omits NaNO3.

Composition

flowchart LR
    CultureMech_000028["bg_11_n_medium"]
    ing0_dH2O["dH2O"]
    CultureMech_000028 --> ing0_dH2O
    CHEBI_15377["CHEBI:15377"]
    ing0_dH2O -.-> CHEBI_15377
    ing1_K2HPO4["K2HPO4"]
    CultureMech_000028 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000028 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000028 --> ing3_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing4_Citric_Acid_x_H2O["Citric Acid x H2O"]
    CultureMech_000028 --> ing4_Citric_Acid_x_H2O
    CHEBI_30769["CHEBI:30769"]
    ing4_Citric_Acid_x_H2O -.-> CHEBI_30769
    ing5_Ferric_Ammonium_Citrate["Ferric Ammonium Citrate"]
    CultureMech_000028 --> ing5_Ferric_Ammonium_Citrate
    CHEBI_31604["CHEBI:31604"]
    ing5_Ferric_Ammonium_Citrate -.-> CHEBI_31604
    ing6_Na2EDTA_x_2_H2O["Na2EDTA x 2 H2O"]
    CultureMech_000028 --> ing6_Na2EDTA_x_2_H2O
    CHEBI_64758["CHEBI:64758"]
    ing6_Na2EDTA_x_2_H2O -.-> CHEBI_64758
    ing7_Na2CO3["Na2CO3"]
    CultureMech_000028 --> ing7_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing7_Na2CO3 -.-> CHEBI_29377

Ingredients (8)

# Ingredient Concentration Ontology Notes
1 dH2O
H2O 18.015 g/mol
1000 ML_PER_L CHEBI:15377
water
 UTEX starts the 1 L BG-11(-N) liquid recipe with approximately 900 mL dH2O.
2 K2HPO4
HO4P.2K 174.174 g/mol
Sigma P 3786
0.22 MILLIMOLAR CHEBI:131527
dipotassium hydrogen phosphate
 UTEX adds 10 mL/L of a 0.8 g/200 mL stock, for 0.22 mM final.
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
Sigma 230391
0.3 MILLIMOLAR CHEBI:31795
magnesium sulfate heptahydrate
 UTEX adds 10 mL/L of a 1.5 g/200 mL stock, for 0.3 mM final.
4 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
Sigma C-3881
0.24 MILLIMOLAR CHEBI:86158
calcium chloride dihydrate
 UTEX adds 10 mL/L of a 0.72 g/200 mL stock, for 0.24 mM final.
5 Citric Acid x H2O
C6H8O7 192.123 g/mol
Fisher A 104
0.012 MILLIMOLAR CHEBI:30769
citric acid
 UTEX adds 10 mL/L of a 0.12 g/200 mL stock, for 0.012 mM final.
6 Ferric Ammonium Citrate 0.02 MILLIMOLAR CHEBI:31604
ferric ammonium citrate
 UTEX adds 10 mL/L of a 0.12 g/200 mL stock, for 0.02 mM final.
7 Na2EDTA x 2 H2O
C10H14N2O8.2H2O.2Na 372.238 g/mol
Sigma ED255
0.002 MILLIMOLAR CHEBI:64758
EDTA disodium salt dihydrate
 UTEX adds 10 mL/L of a 0.02 g/200 mL stock, for 0.002 mM final.
8 Na2CO3
CO3.2Na 105.988 g/mol
Baker 3604
0.18 MILLIMOLAR CHEBI:29377
sodium carbonate
 UTEX adds 10 mL/L of a 0.4 g/200 mL stock, for 0.18 mM final.

Preparation

  1. To approximately 900 mL dH2O, add the first 7 BG-11(-N) mineral stocks and BG-11 Trace Metals Solution while stirring.
  2. Bring the total volume to 1 L with dH2O.
  3. Cover and autoclave the medium.
  4. Allow to cool, then store at refrigerator temperature.

Solutions

BG-11 Trace Metals Solution

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
dH2O 1000 ML_PER_L UTEX brings BG-11 Trace Metals Solution to 1 L with dH2O.
H3BO3 2.86 G_PER_L UTEX lists 2.86 g/L H3BO3, for 46 mM in the BG-11 Trace Metals stock.
MnCl2 x 4 H2O 1.81 G_PER_L UTEX lists 1.81 g/L MnCl2 x 4 H2O, for 9 mM in the BG-11 Trace Metals stock.
ZnSO4 x 7 H2O 0.22 G_PER_L UTEX lists 0.22 g/L ZnSO4 x 7 H2O, for 0.77 mM in the BG-11 Trace Metals stock.
Na2MoO4 x 2 H2O 0.39 G_PER_L UTEX lists 0.39 g/L Na2MoO4 x 2 H2O, for 1.6 mM in the BG-11 Trace Metals stock.
CuSO4 x 5 H2O 0.079 G_PER_L UTEX lists 0.079 g/L CuSO4 x 5 H2O, for 0.3 mM in the BG-11 Trace Metals stock.
Co(NO3)2 x 6 H2O 49.4 MG_PER_L UTEX lists 49.4 mg/L Co(NO3)2 x 6 H2O, for 0.17 mM in the BG-11 Trace Metals stock.
UTEX adds 1 mL/L BG-11 Trace Metals Solution.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-28T00:00:00+00:00 utex-import Imported from UTEX Culture Collection Source ID: bg-11-n-medium, URL: https://utex.org/products/bg-11-n-medium
2026-03-13T06:18:52.937526Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000028
2026-03-17T19:45:03.902697+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "BG-11(-N) Medium" to "bg_11_n_medium" for consistent matching
2026-05-16T09:02:56.384685+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1; references.reference_id->reference=2; category->lowercase=1
2026-05-16T09:11:01.548742+00:00 migrate_preparation_steps.py MIGRATED_PREPARATION_STEPS instruction->action+description for 9 step(s)
2026-08-30T00:58:31.323761+00:00 repair_utex_ingredients.py REPAIRED_UTEX_COMPOSITION Restored 9 ingredient name(s) and amount(s) from a re-fetched UTEX capture. The original fetcher read fixed column positions, so the row ordinal was stored as the ingredient name, the component name as the amount, and the Amount column was never read; concentration was then filled in as G_PER_L regardless. Source: https://utex.org/pages/algal-culture-media, captured 2026-08-29T17:46:40.207779.
2026-09-07T00:00:00-07:00 repair_utex_a_bg11_score30.py RESOLVED_UTEX_A_BG11_SCORE30 UTEX prepares liquid BG-11(-N) Medium from seven mineral stocks and BG-11 Trace Metals Solution diluted in dH2O before autoclaving; this nitrogen-free BG-11 recipe omits NaNO3.