bg_11_medium (CultureMech:000029)

Overview

External ontology

UTEX:bg-11-medium (source link unavailable) — BG-11 Medium

Applications

Algae cultivation
Phytoplankton culture
Microalgae research

Notes

UTEX prepares liquid BG-11 Medium from eight mineral stocks and BG-11 Trace Metals Solution diluted in dH2O before autoclaving.

Composition

flowchart LR
    CultureMech_000029["bg_11_medium"]
    ing0_dH2O["dH2O"]
    CultureMech_000029 --> ing0_dH2O
    CHEBI_15377["CHEBI:15377"]
    ing0_dH2O -.-> CHEBI_15377
    ing1_NaNO3["NaNO3"]
    CultureMech_000029 --> ing1_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing1_NaNO3 -.-> CHEBI_63005
    ing2_K2HPO4["K2HPO4"]
    CultureMech_000029 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000029 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000029 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_Citric_Acid_x_H2O["Citric Acid x H2O"]
    CultureMech_000029 --> ing5_Citric_Acid_x_H2O
    CHEBI_30769["CHEBI:30769"]
    ing5_Citric_Acid_x_H2O -.-> CHEBI_30769
    ing6_Ferric_Ammonium_Citrate["Ferric Ammonium Citrate"]
    CultureMech_000029 --> ing6_Ferric_Ammonium_Citrate
    CHEBI_31604["CHEBI:31604"]
    ing6_Ferric_Ammonium_Citrate -.-> CHEBI_31604
    ing7_Na2EDTA_x_2_H2O["Na2EDTA x 2 H2O"]
    CultureMech_000029 --> ing7_Na2EDTA_x_2_H2O
    CHEBI_64758["CHEBI:64758"]
    ing7_Na2EDTA_x_2_H2O -.-> CHEBI_64758
    ing8_Na2CO3["Na2CO3"]
    CultureMech_000029 --> ing8_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing8_Na2CO3 -.-> CHEBI_29377

Ingredients (9)

#	Ingredient	Concentration	Ontology	Notes
1	dH2O H2O 18.015 g/mol	1000 ML_PER_L	CHEBI:15377 water	UTEX starts the 1 L BG-11 liquid recipe with approximately 900 mL dH2O.
2	NaNO3 NO3.Na 84.994 g/mol Fisher BP360-500	17.6 MILLIMOLAR	CHEBI:63005 sodium nitrate	UTEX adds 10 mL/L of a 30 g/200 mL stock, for 17.6 mM final.
3	K2HPO4 HO4P.2K 174.174 g/mol Sigma P 3786	0.23 MILLIMOLAR	CHEBI:131527 dipotassium hydrogen phosphate	UTEX adds 10 mL/L of a 0.8 g/200 mL stock, for 0.23 mM final.
4	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol Sigma 230391	0.3 MILLIMOLAR	CHEBI:31795 magnesium sulfate heptahydrate	UTEX adds 10 mL/L of a 1.5 g/200 mL stock, for 0.3 mM final.
5	CaCl2 x 2 H2O Ca.2Cl.2H2O 147.014 g/mol Sigma C-3881	0.24 MILLIMOLAR	CHEBI:86158 calcium chloride dihydrate	UTEX adds 10 mL/L of a 0.72 g/200 mL stock, for 0.24 mM final.
6	Citric Acid x H2O C6H8O7 192.123 g/mol Fisher A 104	0.031 MILLIMOLAR	CHEBI:30769 citric acid	UTEX adds 10 mL/L of a 0.12 g/200 mL stock, for 0.031 mM final.
7	Ferric Ammonium Citrate	0.021 MILLIMOLAR	CHEBI:31604 ferric ammonium citrate	UTEX adds 10 mL/L of a 0.12 g/200 mL stock, for 0.021 mM final.
8	Na2EDTA x 2 H2O C10H14N2O8.2H2O.2Na 372.238 g/mol Sigma ED255	0.0027 MILLIMOLAR	CHEBI:64758 EDTA disodium salt dihydrate	UTEX adds 10 mL/L of a 0.02 g/200 mL stock, for 0.0027 mM final.
9	Na2CO3 CO3.2Na 105.988 g/mol Baker 3604	0.19 MILLIMOLAR	CHEBI:29377 sodium carbonate	UTEX adds 10 mL/L of a 0.4 g/200 mL stock, for 0.19 mM final.

Preparation

To approximately 900 mL dH2O, add the first 8 BG-11 mineral stocks and BG-11 Trace Metals Solution while stirring.
Bring the total volume to 1 L with dH2O.
Cover and autoclave the medium.
Allow to cool, then store at refrigerator temperature.

Solutions

BG-11 Trace Metals Solution

Dose: 1 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
dH2O	1000 ML_PER_L	UTEX brings BG-11 Trace Metals Solution to 1 L with dH2O.
H3BO3	2.86 G_PER_L	UTEX lists 2.86 g/L H3BO3, for 46 mM in the BG-11 Trace Metals stock.
MnCl2 x 4 H2O	1.81 G_PER_L	UTEX lists 1.81 g/L MnCl2 x 4 H2O, for 9 mM in the BG-11 Trace Metals stock.
ZnSO4 x 7 H2O	0.22 G_PER_L	UTEX lists 0.22 g/L ZnSO4 x 7 H2O, for 0.77 mM in the BG-11 Trace Metals stock.
Na2MoO4 x 2 H2O	0.39 G_PER_L	UTEX lists 0.39 g/L Na2MoO4 x 2 H2O, for 1.6 mM in the BG-11 Trace Metals stock.
CuSO4 x 5 H2O	0.079 G_PER_L	UTEX lists 0.079 g/L CuSO4 x 5 H2O, for 0.3 mM in the BG-11 Trace Metals stock.
Co(NO3)2 x 6 H2O	49.4 MG_PER_L	UTEX lists 49.4 mg/L Co(NO3)2 x 6 H2O, for 0.17 mM in the BG-11 Trace Metals stock.

UTEX adds 1 mL/L BG-11 Trace Metals Solution.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-28T00:00:00+00:00	utex-import	Imported from UTEX Culture Collection	Source ID: bg-11-medium, URL: https://utex.org/products/bg-11-medium
2026-03-13T06:18:52.945671Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:000029
2026-03-17T19:45:03.909331+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "BG-11 Medium" to "bg_11_medium" for consistent matching
2026-05-16T09:02:56.377266+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=1; references.reference_id->reference=2; category->lowercase=1
2026-05-16T09:11:01.540618+00:00	migrate_preparation_steps.py	MIGRATED_PREPARATION_STEPS	instruction->action+description for 9 step(s)
2026-08-30T00:58:31.309609+00:00	repair_utex_ingredients.py	REPAIRED_UTEX_COMPOSITION	Restored 10 ingredient name(s) and amount(s) from a re-fetched UTEX capture. The original fetcher read fixed column positions, so the row ordinal was stored as the ingredient name, the component name as the amount, and the Amount column was never read; concentration was then filled in as G_PER_L regardless. Source: https://utex.org/pages/algal-culture-media, captured 2026-08-29T17:46:40.207779.
2026-09-05T00:00:00-07:00	repair_utex_bg11_spirulina.py	LINKED_UTEX_BG11_NACL_CHILDREN	Added reciprocal parent-child links for the UTEX BG-11 NaCl variants.
2026-09-07T00:00:00-07:00	repair_utex_a_bg11_score30.py	RESOLVED_UTEX_A_BG11_SCORE30	UTEX prepares liquid BG-11 Medium from eight mineral stocks and BG-11 Trace Metals Solution diluted in dH2O before autoclaving.