bristol_medium (CultureMech:000037)

Overview

External ontology

UTEX:bristol-medium (source link unavailable) — Bristol Medium

Applications

Algae cultivation
Phytoplankton culture
Microalgae research

Notes

UTEX prepares Bristol Medium from six mineral stocks diluted in dH2O and autoclaved.

Composition

flowchart LR
    CultureMech_000037["bristol_medium"]
    ing0_dH2O["dH2O"]
    CultureMech_000037 --> ing0_dH2O
    CHEBI_15377["CHEBI:15377"]
    ing0_dH2O -.-> CHEBI_15377
    ing1_NaNO3["NaNO3"]
    CultureMech_000037 --> ing1_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing1_NaNO3 -.-> CHEBI_63005
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000037 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000037 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_K2HPO4["K2HPO4"]
    CultureMech_000037 --> ing4_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing4_K2HPO4 -.-> CHEBI_131527
    ing5_KH2PO4["KH2PO4"]
    CultureMech_000037 --> ing5_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing5_KH2PO4 -.-> CHEBI_63036
    ing6_NaCl["NaCl"]
    CultureMech_000037 --> ing6_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing6_NaCl -.-> CHEBI_26710

Ingredients (7)

#	Ingredient	Concentration	Ontology	Notes
1	dH2O H2O 18.015 g/mol		CHEBI:15377 water	UTEX starts the 1 L Bristol Medium recipe with approximately 900 mL dH2O.
2	NaNO3 NO3.Na 84.994 g/mol Fisher BP360-500	2.94 MILLIMOLAR	CHEBI:63005 sodium nitrate	UTEX adds 10 mL/L of a 10 g/400 mL dH2O stock, for 2.94 mM final.
3	CaCl2 x 2 H2O Ca.2Cl.2H2O 147.014 g/mol Sigma C-3881	0.17 MILLIMOLAR	CHEBI:86158 calcium chloride dihydrate	UTEX adds 10 mL/L of a 1 g/400 mL dH2O stock, for 0.17 mM final.
4	MgSO4 x 7 H2O 7H2O.Mg.O4S 246.473 g/mol Sigma 230391	0.3 MILLIMOLAR	CHEBI:31795 magnesium sulfate heptahydrate	UTEX adds 10 mL/L of a 3 g/400 mL dH2O stock, for 0.3 mM final.
5	K2HPO4 HO4P.2K 174.174 g/mol Sigma P 3786	0.43 MILLIMOLAR	CHEBI:131527 dipotassium hydrogen phosphate	UTEX adds 10 mL/L of a 3 g/400 mL dH2O stock, for 0.43 mM final.
6	KH2PO4 H2O4P.K 136.084 g/mol Sigma P 0662	1.29 MILLIMOLAR	CHEBI:63036 potassium dihydrogen phosphate	UTEX adds 10 mL/L of a 7 g/400 mL dH2O stock, for 1.29 mM final.
7	NaCl Cl.Na 58.443 g/mol Fisher S271-500	0.43 MILLIMOLAR	CHEBI:26710 sodium chloride	UTEX adds 10 mL/L of a 1 g/400 mL dH2O stock, for 0.43 mM final.

Preparation

To approximately 900 mL dH2O, add each Bristol mineral stock while stirring continuously.
Bring the total volume to 1 L with dH2O.
Cover and autoclave the medium.
Store at refrigerator temperature.

Target organisms and growth evidence

These are curated observations and conditions for named organisms; they do not imply universal growth suitability.

Preferred term
Chlorella pyrenoidosa
Term
Id
NCBITaxon:3077
Label
Chlorella vulgaris
Growth metrics
- Measurement conditions
  Commercial Bristol medium comparator in settled and activated sewage growth study.
  Evidence
  Reference
  PMID:15092268
  Supports
  SUPPORT
  Snippet
  Good growth was obtained in both types of wastewater and the algal production was comparable to and even higher than that found in commercial Bristol medium.
  Explanation
  Literature evidence supports qualitative growth of Chlorella pyrenoidosa on commercial Bristol medium as a comparator.
  Is max attainment
  False

Provenance

Timestamp	Curator	Action	Notes
2026-01-28	utex-import	Imported from UTEX Culture Collection	Source ID: bristol-medium, URL: https://utex.org/products/bristol-medium
2026-03-13T06:18:52.988824Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:000037
2026-03-17T19:45:03.946761+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "Bristol Medium" to "bristol_medium" for consistent matching
2026-08-06T00:58:02.502002+00:00	curate_organism_culture_type.py	SET_ORGANISM_CULTURE_TYPE	Inferred isolate: 1 specific strain(s) named, no community signal (#142)
2026-08-30T00:58:30.648112+00:00	repair_utex_ingredients.py	REPAIRED_UTEX_COMPOSITION	Restored 6 ingredient name(s) and amount(s) from a re-fetched UTEX capture. The original fetcher read fixed column positions, so the row ordinal was stored as the ingredient name, the component name as the amount, and the Amount column was never read; concentration was then filled in as G_PER_L regardless. Source: https://utex.org/pages/algal-culture-media, captured 2026-08-29T17:46:40.207779.
2026-09-06T00:00:00-07:00	repair_utex_algal_blends.py	LINKED_UTEX_ALGAL_BLEND_CHILDREN	Added or refreshed reciprocal parent-child link for algae/Botryococcus_Medium.yaml.
2026-09-06T00:00:00-07:00	repair_utex_remaining_score50.py	LINKED_UTEX_SCORE50_ALGAE_CHILDREN	Added or refreshed reciprocal parent-child link for algae/Proteose_Medium.yaml.
2026-09-06T00:00:00-07:00	repair_utex_remaining_score50.py	LINKED_UTEX_SCORE50_ALGAE_CHILDREN	Added or refreshed reciprocal parent-child link for algae/bristol_nacl_medium.yaml.
2026-09-06T00:00:00-07:00	repair_algae_score45_blends_soils.py	LINKED_SCORE45_ALGAE_CHILDREN	Added or refreshed reciprocal parent-child link for algae/Soil_Extract_Medium.yaml.
2026-09-06T00:00:00-07:00	repair_utex_bristol_bold_score35.py	RESOLVED_UTEX_BRISTOL_BOLD_SCORE35	UTEX prepares Bristol Medium from six mineral stocks diluted in dH2O and autoclaved.
2026-10-10T03:53:28.828041+00:00	codex	CURATED_UTEX_BRISTOL_BBM_INGREDIENT_AND_PREPARATION_EVIDENCE	The BBM trace source prints MnCl with tetrahydrate; its intended chemical identity and the existing MnCl2 amount remain unverified. Added node evidence supports only that source-spelling conflict. BBM trace concentration columns and stock-page parenthetical micromolar values conflict with explicit stock preparation amounts. Preserve inspected Amount-per-litre stock recipes, not those incompatible columns; no new final-medium conversions. The BBM iron stock does not specify acid strength. No density, purity or molarity is assumed. The BBM study cites Stein 1973; exact equivalence with the current UTEX parent is unverified. NH4Cl ppm basis and culture working volume remain unspecified; no numerical conversion or aliquot inference. Bristol-NaCl uses an unfinished parent preparation; its table one-litre row is not certified as a working dose of completed Bristol. The reference and explicit stage notes are retained without a fabricated dose. Optional agar formulations are not silently added to these liquid records. No new taxa-media associations, IDs or growth/role assertions are curated. Supplier-catalog identities, generic incubation/light claims and unrelated preexisting organism evidence are not blanket-certified by this ingredient review.