2asw (CultureMech:000038)

Overview

Applications

Algae cultivation
Phytoplankton culture
Microalgae research

Notes

Full recipe available at https://www.ccap.ac.uk/wp-content/uploads/MR_2ASW.pdf

Composition

flowchart LR
    CultureMech_000038["2asw"]
    ing0_NaNO3["NaNO3"]
    CultureMech_000038 --> ing0_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing0_NaNO3 -.-> CHEBI_63005
    ing1_Na2HPO4["Na2HPO4"]
    CultureMech_000038 --> ing1_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing1_Na2HPO4 -.-> CHEBI_34683
    ing2_K2HPO4["K2HPO4"]
    CultureMech_000038 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_Biotin["Biotin"]
    CultureMech_000038 --> ing3_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing3_Biotin -.-> CHEBI_15956
    ing4_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_000038 --> ing4_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing4_Calcium_pantothenate -.-> CHEBI_31345
    ing5_Cyanocobalamin["Cyanocobalamin"]
    CultureMech_000038 --> ing5_Cyanocobalamin
    CHEBI_17439["CHEBI:17439"]
    ing5_Cyanocobalamin -.-> CHEBI_17439
    ing6_Folic_acid["Folic acid"]
    CultureMech_000038 --> ing6_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing6_Folic_acid -.-> CHEBI_27470
    ing7_Inositol["Inositol"]
    CultureMech_000038 --> ing7_Inositol
    CHEBI_17268["CHEBI:17268"]
    ing7_Inositol -.-> CHEBI_17268
    ing8_Nicotinic_acid["Nicotinic acid"]
    CultureMech_000038 --> ing8_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing8_Nicotinic_acid -.-> CHEBI_15940
    ing9_Thiamine_HCl["Thiamine HCl"]
    CultureMech_000038 --> ing9_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing9_Thiamine_HCl -.-> CHEBI_49105
    ing10_Thymine["Thymine"]
    CultureMech_000038 --> ing10_Thymine
    CHEBI_17821["CHEBI:17821"]
    ing10_Thymine -.-> CHEBI_17821
    ing11_Tricine["Tricine"]
    CultureMech_000038 --> ing11_Tricine
    CHEBI_46760["CHEBI:46760"]
    ing11_Tricine -.-> CHEBI_46760
    ing12_NaCl["NaCl"]
    CultureMech_000038 --> ing12_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing12_NaCl -.-> CHEBI_26710

Ingredients (13)

#	Ingredient	Concentration	Ontology	Notes
1	NaNO3 NO3.Na 84.994 g/mol	15.00 G_PER_L	CHEBI:63005 sodium nitrate	From CCAP Medium MR_2ASW.pdf; Curated from CCAP Medium MR_2ASW.pdf specification; Identity verified against CCAP MR_2ASW.pdf revision 002, approved/reviewed 2026-05-29. The retained 15.00 g/L is the extra-salts stock concentration, not the final medium concentration.
2	Na2HPO4 HO4P.2Na 141.958 g/mol	0.60 G_PER_L	CHEBI:34683 disodium hydrogenphosphate	From CCAP Medium MR_2ASW.pdf; Curated from CCAP Medium MR_2ASW.pdf specification
3	K2HPO4 HO4P.2K 174.174 g/mol	0.50 G_PER_L	CHEBI:131527 dipotassium hydrogen phosphate	From CCAP Medium MR_2ASW.pdf; Curated from CCAP Medium MR_2ASW.pdf specification
4	Biotin C10H16N2O3S 244.316 g/mol	0.0002 G_PER_L	CHEBI:15956 biotin	From CCAP Medium MR_2ASW.pdf; Curated from CCAP Medium MR_2ASW.pdf specification
5	Calcium pantothenate 2C9H16NO5.Ca 476.536 g/mol	0.02 G_PER_L	CHEBI:31345 Calcium pantothenate	From CCAP Medium MR_2ASW.pdf; Curated from CCAP Medium MR_2ASW.pdf specification
6	Cyanocobalamin C63H88CoN14O14P 1355.388 g/mol	0.004 G_PER_L	CHEBI:17439 cyanocob(III)alamin	From CCAP Medium MR_2ASW.pdf; Curated from CCAP Medium MR_2ASW.pdf specification
7	Folic acid C19H19N7O6 441.404 g/mol	0.0004 G_PER_L	CHEBI:27470 folic acid	From CCAP Medium MR_2ASW.pdf; Curated from CCAP Medium MR_2ASW.pdf specification
8	Inositol C6H12O6 180.156 g/mol	1.0 G_PER_L	CHEBI:17268 myo-inositol	From CCAP Medium MR_2ASW.pdf; Curated from CCAP Medium MR_2ASW.pdf specification
9	Nicotinic acid C6H5NO2 123.111 g/mol	0.02 G_PER_L	CHEBI:15940 nicotinic acid	From CCAP Medium MR_2ASW.pdf; Curated from CCAP Medium MR_2ASW.pdf specification
10	Thiamine HCl C12H17N4OS.Cl.HCl 337.276 g/mol	0.1 G_PER_L	CHEBI:49105 thiamine hydrochloride	From CCAP Medium MR_2ASW.pdf; Curated from CCAP Medium MR_2ASW.pdf specification
11	Thymine C5H6N2O2 126.115 g/mol	0.6 G_PER_L	CHEBI:17821 thymine	From CCAP Medium MR_2ASW.pdf; Curated from CCAP Medium MR_2ASW.pdf specification
12	Tricine	0.50 G_PER_L	CHEBI:46760 tricine	From CCAP Medium MR_2ASW.pdf; Curated from CCAP Medium MR_2ASW.pdf specification
13	NaCl Cl.Na 58.443 g/mol	35.00 G_PER_L	CHEBI:26710 sodium chloride	From CCAP Medium MR_2ASW.pdf; Curated from CCAP Medium MR_2ASW.pdf specification

Target organisms and growth evidence

These are curated observations and conditions for named organisms; they do not imply universal growth suitability.

Preferred term
Dunaliella salina
Term
Id
NCBITaxon:3046
Label
Dunaliella salina
Strain
CCAP 19/18
Growth metrics
- Measurement conditions
  CCAP strain maintenance in 2ASW; axenic serial subculture at 20-35 deg C.
  Evidence
  Reference
  https://www.ccap.ac.uk/catalogue/strain-19-18
  Supports
  SUPPORT
  Explanation
  CCAP directly links Dunaliella salina CCAP 19/18 to 2ASW maintenance culture conditions.
Evidence
- Reference
  https://www.ccap.ac.uk/catalogue/strain-19-18
  Supports
  SUPPORT
  Explanation
  The CCAP culture collection strain page explicitly lists 2ASW as the maintenance medium for Dunaliella salina CCAP 19/18.
- Reference
  NCBI:PRJNA32771
  Supports
  SUPPORT
  Explanation
  NCBI BioProject PRJNA32771 links the same strain to Taxonomy ID 3046, assembly GCA_002284615.2, WGS NSFN00000000, and BioSample SAMN02746051.
Preferred term
Phaeodactylum tricornutum
Term
Id
NCBITaxon:556484
Label
Phaeodactylum tricornutum CCAP 1055/1
Strain
UTEX 642
Growth metrics
- Measurement conditions
  F/2ASW variant at 20 deg C, continuous illumination of 30-75 umol photons m-2 s-1, and aeration with 1% CO2 or ambient air; reported mid-log harvest OD730 0.2-0.3.
  Evidence
  Reference
  DOI:10.1104/pp.18.00453
  Supports
  SUPPORT
  Explanation
  Matsui et al. explicitly name Phaeodactylum tricornutum UTEX 642 and F/2ASW culture conditions.
Evidence
- Reference
  DOI:10.1104/pp.18.00453
  Supports
  PARTIAL
  Explanation
  The source supports growth on the F/2ASW variant. The NCBI reference genome identifiers available for Phaeodactylum tricornutum are tied to CCAP 1055/1, a closely related collection designation, so the UTEX-to-genome mapping should be treated cautiously.
Preferred term
Thalassiosira pseudonana
Term
Id
NCBITaxon:296543
Label
Thalassiosira pseudonana CCMP1335
Strain
CCMP1335
Growth metrics
- Measurement conditions
  F/2ASW variant at 20 deg C, continuous illumination of 30-75 umol photons m-2 s-1, and aeration with 1% CO2 or ambient air; reported mid-log harvest OD730 0.1-0.15.
  Evidence
  Reference
  DOI:10.1104/pp.18.00453
  Supports
  SUPPORT
  Explanation
  Matsui et al. explicitly name Thalassiosira pseudonana CCMP1335 and F/2ASW culture conditions.
Evidence
- Reference
  DOI:10.1104/pp.18.00453
  Supports
  SUPPORT
  Explanation
  The source supports growth on the F/2ASW variant for the same strain represented by NCBI Taxonomy ID 296543, assembly GCF_000149405.2, WGS AAFD00000000, and BioSample SAMN02744045.
Preferred term
Chaetoceros gracilis
Strain
UTEX LB2658
Growth metrics
- Measurement conditions
  Modified F/2ASW sodium-response variant supplemented with sodium selenite and sodium metasilicate; sodium concentration adjusted by NaCl addition and CO2 availability varied.
  Evidence
  Reference
  DOI:10.1007/s10126-021-10037-4
  Supports
  SUPPORT
  Explanation
  Tsuji et al. report Chaetoceros gracilis growth dependency on sodium and CO2 using a modified F/2ASW formulation.
Evidence
- Reference
  DOI:10.1007/s10126-021-10037-4
  Supports
  SUPPORT
  Explanation
  The study identifies the UTEX LB2658 strain and uses modified F/2ASW to test growth under sodium and CO2 conditions.

Recipe variants

Name
f_2asw
Description
Artificial seawater supplemented with half-strength Guillard f nutrients for marine diatom culture.
Modifications
- Supplement the artificial seawater base with half-strength Guillard f solution.
- Use liquid or agar-solidified F/2ASW depending on maintenance, transformation, or selection protocol.
- Reported diatom conditions include 20 deg C, continuous illumination at 30-75 umol photons m-2 s-1, and aeration with ambient air or CO2-enriched air.
Purpose
Capture F/2 nutrient-supplemented artificial seawater as a recognizable variant of the 2ASW parent formulation.
Evidence
- Reference
  DOI:10.1104/pp.18.00453
  Supports
  SUPPORT
  Explanation
  Matsui et al. explicitly define F/2ASW as artificial seawater plus half-strength Guillard f solution for Phaeodactylum tricornutum UTEX 642 and Thalassiosira pseudonana CCMP1335 cultivation.
Name
modified_f_2asw_sodium_response
Description
Modified F/2 artificial seawater used to assay sodium and CO2 dependence in Chaetoceros gracilis UTEX LB2658.
Modifications
- Supplement F/2ASW with 10 nM sodium selenite and 0.1 mM sodium metasilicate.
- Manipulate sodium by changing NaCl added to the basal artificial seawater.
- Preacclimate cells to sodium concentrations before low-sodium transfer.
Purpose
Model experimental sodium-response medium variants without creating a duplicate artificial seawater parent record.
Evidence
- Reference
  DOI:10.1007/s10126-021-10037-4
  Supports
  SUPPORT
  Explanation
  Tsuji et al. report Chaetoceros gracilis UTEX LB2658 growth and carbon-concentrating-mechanism experiments in modified F/2ASW with defined sodium manipulations.

Provenance

Timestamp	Curator	Action	Notes
2026-01-28	ccap-import	Imported from CCAP Culture Collection	Source ID: 2ASW, PDF URL: https://www.ccap.ac.uk/wp-content/uploads/MR_2ASW.pdf
2026-02-03T11:23:39.554565	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added placeholder ingredients for missing composition
2026-02-03T11:26:43.440568	quality-tagger-v1.0	Added data quality flag	Marked as incomplete_composition due to placeholder ingredients
2026-03-13T06:18:52.992431Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:000038
2026-03-17T19:45:03.950098+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "2ASW" to "2asw" for consistent matching
2026-04-04T12:54:55.050840	batch_process_collection_media	EXPANDED_INGREDIENTS	Replaced placeholder with 13 curated ingredients from CCAP Medium MR_2ASW.pdf.
2026-05-09T13:35:00-07:00	culturemech-agentic-curation	ADDED_GROWTH_EVIDENCE	Added strain-specific 2ASW growth evidence from CCAP and NCBI records, plus F/2ASW variant evidence from peer-reviewed diatom culture studies.
2026-06-10T07:37:33.980020+00:00	primary-term-regrounding-v1.0	Re-grounded wrong primary term id (OAK-verified)	1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T00:58:02.481264+00:00	curate_organism_culture_type.py	SET_ORGANISM_CULTURE_TYPE	Inferred isolate: 4 specific strain(s) named, no community signal (#142)
2026-09-13T00:00:00-07:00	repair_ccap_sources_score10.py	NORMALIZED_CCAP_SOURCE_SCORE10	Copied the CCAP source identity from references and the original ccap-import event into the structured sources slot recognized by review scoring.
2026-10-05T19:29:05+00:00	codex	CORRECTED_INGREDIENT_IDENTITY	Verified current CCAP revision 002 (approved/reviewed 2026-05-29), retrieved 2026-10-05, SHA256 d5d658702a3640ed17c4d9d494f198f7122be90cbc81e64ccdc8afad1c4401cc. The April ingredient import predates this revision; the original imported PDF bytes are not retained in the tracked corpus, so historical version equivalence is not asserted. Current recipe explicitly lists sodium nitrate in extra-salts stock. ChEBI:63005 identity and packaged MediaIngredientMech NaNO3 mapping agree. No other ingredient identities or concentrations were changed.