asw_barley (CultureMech:000043)

Overview

Applications

Algae cultivation
Phytoplankton culture
Microalgae research

Notes

Full recipe available at https://www.ccap.ac.uk/wp-content/uploads/MR_ASW_Barley.pdf

Composition

flowchart LR
    CultureMech_000043["asw_barley"]
    ing0_NaNO["NaNO"]
    CultureMech_000043 --> ing0_NaNO
    CHEBI_34545["CHEBI:34545"]
    ing0_NaNO -.-> CHEBI_34545
    ing1_Na2HPO4["Na2HPO4"]
    CultureMech_000043 --> ing1_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing1_Na2HPO4 -.-> CHEBI_34683
    ing2_K2HPO4["K2HPO4"]
    CultureMech_000043 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_Biotin["Biotin"]
    CultureMech_000043 --> ing3_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing3_Biotin -.-> CHEBI_15956
    ing4_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_000043 --> ing4_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing4_Calcium_pantothenate -.-> CHEBI_31345
    ing5_Cyanocobalamin["Cyanocobalamin"]
    CultureMech_000043 --> ing5_Cyanocobalamin
    CHEBI_17439["CHEBI:17439"]
    ing5_Cyanocobalamin -.-> CHEBI_17439
    ing6_Folic_acid["Folic acid"]
    CultureMech_000043 --> ing6_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing6_Folic_acid -.-> CHEBI_27470
    ing7_Inositol["Inositol"]
    CultureMech_000043 --> ing7_Inositol
    CHEBI_17268["CHEBI:17268"]
    ing7_Inositol -.-> CHEBI_17268
    ing8_Nicotinic_acid["Nicotinic acid"]
    CultureMech_000043 --> ing8_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing8_Nicotinic_acid -.-> CHEBI_15940
    ing9_Thiamine_HCl["Thiamine HCl"]
    CultureMech_000043 --> ing9_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing9_Thiamine_HCl -.-> CHEBI_49105
    ing10_Thymine["Thymine"]
    CultureMech_000043 --> ing10_Thymine
    CHEBI_17821["CHEBI:17821"]
    ing10_Thymine -.-> CHEBI_17821
    ing11_Tricine["Tricine"]
    CultureMech_000043 --> ing11_Tricine
    CHEBI_46760["CHEBI:46760"]
    ing11_Tricine -.-> CHEBI_46760
    ing12_Barley_grain["Barley grain"]
    CultureMech_000043 --> ing12_Barley_grain

Ingredients (13)

#	Ingredient	Concentration	Ontology	Notes
1	NaNO C23H28ClN5O3 457.962 g/mol	15.00 G_PER_L	CHEBI:34545 Azimilide	From CCAP Medium MR_ASW_Barley.pdf; Curated from CCAP Medium MR_ASW_Barley.pdf specification
2	Na2HPO4 HO4P.2Na 141.958 g/mol	0.60 G_PER_L	CHEBI:34683 disodium hydrogenphosphate	From CCAP Medium MR_ASW_Barley.pdf; Curated from CCAP Medium MR_ASW_Barley.pdf specification
3	K2HPO4 HO4P.2K 174.174 g/mol	0.50 G_PER_L	CHEBI:131527 dipotassium hydrogen phosphate	From CCAP Medium MR_ASW_Barley.pdf; Curated from CCAP Medium MR_ASW_Barley.pdf specification
4	Biotin C10H16N2O3S 244.316 g/mol	0.0002 G_PER_L	CHEBI:15956 biotin	From CCAP Medium MR_ASW_Barley.pdf; Curated from CCAP Medium MR_ASW_Barley.pdf specification
5	Calcium pantothenate 2C9H16NO5.Ca 476.536 g/mol	0.02 G_PER_L	CHEBI:31345 Calcium pantothenate	From CCAP Medium MR_ASW_Barley.pdf; Curated from CCAP Medium MR_ASW_Barley.pdf specification
6	Cyanocobalamin C63H88CoN14O14P 1355.388 g/mol	0.004 G_PER_L	CHEBI:17439 cyanocob(III)alamin	From CCAP Medium MR_ASW_Barley.pdf; Curated from CCAP Medium MR_ASW_Barley.pdf specification
7	Folic acid C19H19N7O6 441.404 g/mol	0.0004 G_PER_L	CHEBI:27470 folic acid	From CCAP Medium MR_ASW_Barley.pdf; Curated from CCAP Medium MR_ASW_Barley.pdf specification
8	Inositol C6H12O6 180.156 g/mol	1.0 G_PER_L	CHEBI:17268 myo-inositol	From CCAP Medium MR_ASW_Barley.pdf; Curated from CCAP Medium MR_ASW_Barley.pdf specification
9	Nicotinic acid C6H5NO2 123.111 g/mol	0.02 G_PER_L	CHEBI:15940 nicotinic acid	From CCAP Medium MR_ASW_Barley.pdf; Curated from CCAP Medium MR_ASW_Barley.pdf specification
10	Thiamine HCl C12H17N4OS.Cl.HCl 337.276 g/mol	0.1 G_PER_L	CHEBI:49105 thiamine hydrochloride	From CCAP Medium MR_ASW_Barley.pdf; Curated from CCAP Medium MR_ASW_Barley.pdf specification
11	Thymine C5H6N2O2 126.115 g/mol	0.6 G_PER_L	CHEBI:17821 thymine	From CCAP Medium MR_ASW_Barley.pdf; Curated from CCAP Medium MR_ASW_Barley.pdf specification
12	Tricine	0.50 G_PER_L	CHEBI:46760 tricine	From CCAP Medium MR_ASW_Barley.pdf; Curated from CCAP Medium MR_ASW_Barley.pdf specification
13	Barley grain	variable VARIABLE		Source amount: one barley grain per 25 mL prepared medium; the schema has no count-per-volume unit.

Preparation

Bring to 1 L with filtered natural seawater.
Adjust pH to 7.6-7.8 with 1 M NaOH or 1 M HCl before autoclaving.
Add one barley grain to each 25 mL of prepared medium.
Autoclave at 15 psi for 15 minutes.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-28T00:00:00+00:00	ccap-import	Imported from CCAP Culture Collection	Source ID: ASW_Barley, PDF URL: https://www.ccap.ac.uk/wp-content/uploads/MR_ASW_Barley.pdf
2026-02-03T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added placeholder ingredients for missing composition
2026-02-03T11:26:43.451288	quality-tagger-v1.0	Added data quality flag	Marked as incomplete_composition due to placeholder ingredients
2026-03-13T06:18:53.006321Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:000043
2026-03-17T19:45:03.962563+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "ASW + barley" to "asw_barley" for consistent matching
2026-04-04T12:54:55.176885	batch_process_collection_media	EXPANDED_INGREDIENTS
2026-05-16T08:57:02.115835+00:00	migrate_data_quality_flags.py	MIGRATED_DATA_QUALITY_FLAGS	dict(4 keys) -> list(3 entries)
2026-05-16T09:02:56.256731+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=2; references.reference_id->reference=2; category->lowercase=1
2026-06-10T07:37:33.993168+00:00	primary-term-regrounding-v1.0	Re-grounded wrong primary term id (OAK-verified)	1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-25T00:00:00-07:00	repair_ccap_instruction_rows.py	REPAIRED_CCAP_INSTRUCTION_ROWS	Repaired preparation text and nested-recipe flattening against CCAP MR_ASW_Barley.pdf. Moved process statements to preparation_steps and restored source-asserted components or constituent-medium references where applicable.
2026-09-13T00:00:00-07:00	repair_ccap_sources_score10.py	NORMALIZED_CCAP_SOURCE_SCORE10	Copied the CCAP source identity from references and the original ccap-import event into the structured sources slot recognized by review scoring.