Overview
- Applications
-
- Algae cultivation
- Phytoplankton culture
- Microalgae research
- Notes
- Full recipe available at https://www.ccap.ac.uk/wp-content/uploads/MR_ASW_300.pdf
Composition
flowchart LR
CultureMech_000048["asw_300_barley"]
ing0_NaCl["NaCl"]
CultureMech_000048 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_KCl["KCl"]
CultureMech_000048 --> ing1_KCl
CHEBI_32588["CHEBI:32588"]
ing1_KCl -.-> CHEBI_32588
ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_000048 --> ing2_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
ing3_MgSO__7H_O["MgSO .7H O"]
CultureMech_000048 --> ing3_MgSO__7H_O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO__7H_O -.-> CHEBI_31795
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_000048 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
243.2 G_PER_L |
CHEBI:26710
sodium chloride
|
From CCAP Medium MR_ASW_300.pdf; Curated from CCAP Medium MR_ASW_300.pdf specification |
| 2 |
KCl
Cl.K 74.551 g/mol
|
7.6 G_PER_L |
CHEBI:32588
potassium chloride
|
From CCAP Medium MR_ASW_300.pdf; Curated from CCAP Medium MR_ASW_300.pdf specification |
| 3 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
54.36 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
From CCAP Medium MR_ASW_300.pdf; Curated from CCAP Medium MR_ASW_300.pdf specification |
| 4 |
MgSO .7H O
7H2O.Mg.O4S 246.473 g/mol
|
59.4 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
From CCAP Medium MR_ASW_300.pdf; Curated from CCAP Medium MR_ASW_300.pdf specification |
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1.3 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
From CCAP Medium MR_ASW_300.pdf; Curated from CCAP Medium MR_ASW_300.pdf specification |
Preparation
- Bring to 1 L with deionised water.
- Adjust pH to 7.6-8 with 1 M NaOH or 1 M HCl before autoclaving.
- Autoclave at 15 psi for 15 minutes.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 1 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable). |
| 2026-01-28T00:00:00+00:00 | ccap-import | Imported from CCAP Culture Collection | Source ID: ASW_300, PDF URL: https://www.ccap.ac.uk/wp-content/uploads/MR_ASW_300.pdf |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added placeholder ingredients for missing composition |
| 2026-02-03T11:26:43.461282 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-03-13T06:18:53.020335Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000048 |
| 2026-03-17T19:45:03.976235+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ASW 300 + barley" to "asw_300_barley" for consistent matching |
| 2026-04-04T12:54:54.822495 | batch_process_collection_media | EXPANDED_INGREDIENTS | |
| 2026-05-16T08:57:02.105193+00:00 | migrate_data_quality_flags.py | MIGRATED_DATA_QUALITY_FLAGS | dict(4 keys) -> list(3 entries) |
| 2026-05-16T09:02:56.246904+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2; references.reference_id->reference=2; category->lowercase=1 |
| 2026-06-10T06:57:11.112055+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-08-25T00:00:00-07:00 | repair_ccap_instruction_rows.py | REPAIRED_CCAP_INSTRUCTION_ROWS | Repaired preparation text and nested-recipe flattening against CCAP MR_ASW_300.pdf. Moved process statements to preparation_steps and restored source-asserted components or constituent-medium references where applicable. |
| 2026-09-13T00:00:00-07:00 | repair_ccap_sources_score10.py | NORMALIZED_CCAP_SOURCE_SCORE10 | Copied the CCAP source identity from references and the original ccap-import event into the structured sources slot recognized by review scoring. |