Overview
- Applications
-
- Algae cultivation
- Phytoplankton culture
- Microalgae research
- Notes
- Full recipe available at https://www.ccap.ac.uk/wp-content/uploads/MR_C_Modified.pdf
Composition
flowchart LR
CultureMech_000056["c_medium_modified"]
ing0_Na2EDTA["Na2EDTA"]
CultureMech_000056 --> ing0_Na2EDTA
CHEBI_64734["CHEBI:64734"]
ing0_Na2EDTA -.-> CHEBI_64734
ing1_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_000056 --> ing1_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing1_FeCl3_x_6_H2O -.-> CHEBI_86254
ing2_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_000056 --> ing2_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing2_MnCl2_x_4_H2O -.-> CHEBI_86368
ing3_ZnCl__6H_O["ZnCl .6H O"]
CultureMech_000056 --> ing3_ZnCl__6H_O
CHEBI_49976["CHEBI:49976"]
ing3_ZnCl__6H_O -.-> CHEBI_49976
ing4_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_000056 --> ing4_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing4_CoCl2_x_6_H2O -.-> CHEBI_53503
ing5_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000056 --> ing5_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing5_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Na2EDTA
C10H14N2O8.2Na 336.208 g/mol
|
0.75 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
Na2EDTA in the source-defined trace-element stock; this is a stock composition, not a final-medium concentration. |
| 2 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
0.097 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
From CCAP Medium MR_C_Modified.pdf; Curated from CCAP Medium MR_C_Modified.pdf specification |
| 3 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.041 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
From CCAP Medium MR_C_Modified.pdf; Curated from CCAP Medium MR_C_Modified.pdf specification |
| 4 |
ZnCl .6H O
2Cl.Zn 136.296 g/mol
|
0.005 G_PER_L |
CHEBI:49976
zinc dichloride
|
From CCAP Medium MR_C_Modified.pdf; Curated from CCAP Medium MR_C_Modified.pdf specification |
| 5 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.002 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
From CCAP Medium MR_C_Modified.pdf; Curated from CCAP Medium MR_C_Modified.pdf specification |
| 6 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.004 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
From CCAP Medium MR_C_Modified.pdf; Curated from CCAP Medium MR_C_Modified.pdf specification |
Preparation
- Dissolve Tris in 900 mL distilled water, then add the remaining final-medium components.
- Bring to 1 L with distilled water.
- For an agar formulation, add 15 g/L Bacteriological Agar.
- Ensure the final pH is 7.5.
- Autoclave at 15 psi for 15 minutes.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-28T00:00:00+00:00 | ccap-import | Imported from CCAP Culture Collection | Source ID: C_Modified, PDF URL: https://www.ccap.ac.uk/wp-content/uploads/MR_C_Modified.pdf |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added placeholder ingredients for missing composition |
| 2026-02-03T11:26:43.476328 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-03-13T06:18:53.041868Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000056 |
| 2026-03-17T19:45:03.997075+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "C Medium, Modified" to "c_medium_modified" for consistent matching |
| 2026-04-04T12:54:54.886165 | batch_process_collection_media | EXPANDED_INGREDIENTS | |
| 2026-05-16T08:57:02.225632+00:00 | migrate_data_quality_flags.py | MIGRATED_DATA_QUALITY_FLAGS | dict(4 keys) -> list(3 entries) |
| 2026-05-16T09:02:56.405538+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2; references.reference_id->reference=2; category->lowercase=1 |
| 2026-08-25T00:00:00-07:00 | repair_ccap_instruction_rows.py | REPAIRED_CCAP_INSTRUCTION_ROWS | Repaired preparation text and nested-recipe flattening against CCAP MR_C_Modified.pdf. Moved process statements to preparation_steps and restored source-asserted components or constituent-medium references where applicable. |
| 2026-09-13T00:00:00-07:00 | repair_ccap_sources_score10.py | NORMALIZED_CCAP_SOURCE_SCORE10 | Copied the CCAP source identity from references and the original ccap-import event into the structured sources slot recognized by review scoring. |