cgm

CultureMech:000058 algae COMPLEX LIQUID
Original name: CGM

Overview

Applications
  • Algae cultivation
  • Phytoplankton culture
  • Microalgae research
Notes
Full recipe available at https://www.ccap.ac.uk/wp-content/uploads/MR_CGM.pdf

Composition

flowchart LR
    CultureMech_000058["cgm"]
    ing0_Na_EDTA["Na EDTA"]
    CultureMech_000058 --> ing0_Na_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing0_Na_EDTA -.-> CHEBI_64734
    ing1_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_000058 --> ing1_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing1_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing2_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000058 --> ing2_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing2_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing3_ZnCl2["ZnCl2"]
    CultureMech_000058 --> ing3_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing3_ZnCl2 -.-> CHEBI_49976
    ing4_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000058 --> ing4_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing4_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing5_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000058 --> ing5_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing5_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing6_Proteose_peptone["Proteose peptone"]
    CultureMech_000058 --> ing6_Proteose_peptone
    ing7_Yeast_extract["Yeast extract"]
    CultureMech_000058 --> ing7_Yeast_extract
    ing8_D_glucose["D-glucose"]
    CultureMech_000058 --> ing8_D_glucose
    CHEBI_17634["CHEBI:17634"]
    ing8_D_glucose -.-> CHEBI_17634

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 Na EDTA
C10H14N2O8.2Na 336.208 g/mol
0.75 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
 From CCAP Medium MR_CGM.pdf; Curated from CCAP Medium MR_CGM.pdf specification
2 FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
0.097 G_PER_L CHEBI:86254
iron trichloride hexahydrate
 From CCAP Medium MR_CGM.pdf; Curated from CCAP Medium MR_CGM.pdf specification
3 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.041 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
 From CCAP Medium MR_CGM.pdf; Curated from CCAP Medium MR_CGM.pdf specification
4 ZnCl2
2Cl.Zn 136.296 g/mol
0.005 G_PER_L CHEBI:49976
zinc dichloride
 From CCAP Medium MR_CGM.pdf; Curated from CCAP Medium MR_CGM.pdf specification
5 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.002 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
 From CCAP Medium MR_CGM.pdf; Curated from CCAP Medium MR_CGM.pdf specification
6 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.004 G_PER_L CHEBI:75213
sodium molybdate dihydrate
 From CCAP Medium MR_CGM.pdf; Curated from CCAP Medium MR_CGM.pdf specification
7 Proteose peptone 0.25 G_PER_L MICRO:0000180 (source link unavailable)
Proteose peptone
 From CCAP Medium MR_CGM.pdf; Curated from CCAP Medium MR_CGM.pdf specification
8 Yeast extract 0.25 G_PER_L FOODON:03315426
yeast extract
 From CCAP Medium MR_CGM.pdf; Curated from CCAP Medium MR_CGM.pdf specification
9 D-glucose
C6H12O6 180.156 g/mol
5.00 G_PER_L CHEBI:17634
D-glucose
 From CCAP Medium MR_CGM.pdf; Curated from CCAP Medium MR_CGM.pdf specification

Preparation

  1. Bring to 1 L with deionised water.
  2. For an agar formulation, add 15 g/L Bacteriological Agar.
  3. Autoclave at 15 psi for 15 minutes.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-28T00:00:00+00:00 ccap-import Imported from CCAP Culture Collection Source ID: CGM, PDF URL: https://www.ccap.ac.uk/wp-content/uploads/MR_CGM.pdf
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added placeholder ingredients for missing composition
2026-02-03T11:26:43.480065 quality-tagger-v1.0 Added data quality flag Marked as incomplete_composition due to placeholder ingredients
2026-03-13T06:18:53.047301Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000058
2026-03-17T19:45:04.002509+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "CGM" to "cgm" for consistent matching
2026-04-04T12:54:54.932216 batch_process_collection_media EXPANDED_INGREDIENTS
2026-05-16T08:57:02.235769+00:00 migrate_data_quality_flags.py MIGRATED_DATA_QUALITY_FLAGS dict(4 keys) -> list(3 entries)
2026-05-16T09:02:56.414012+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2; references.reference_id->reference=2; category->lowercase=1
2026-08-25T00:00:00-07:00 repair_ccap_instruction_rows.py REPAIRED_CCAP_INSTRUCTION_ROWS Repaired preparation text and nested-recipe flattening against CCAP MR_CGM.pdf. Moved process statements to preparation_steps and restored source-asserted components or constituent-medium references where applicable.
2026-09-13T00:00:00-07:00 repair_ccap_sources_score10.py NORMALIZED_CCAP_SOURCE_SCORE10 Copied the CCAP source identity from references and the original ccap-import event into the structured sources slot recognized by review scoring.