ch

CultureMech:000059 algae DEFINED LIQUID
Original name: CH

Overview

Applications
  • Algae cultivation
  • Phytoplankton culture
  • Microalgae research
Notes
Full recipe available at https://www.ccap.ac.uk/wp-content/uploads/MR_CH.pdf Composition verified against https://www.ccap.ac.uk/wp-content/uploads/MR_CH.pdf on 2026-08-25.

Composition

flowchart LR
    CultureMech_000059["ch"]
    ing0_Deionized_water["Deionized water"]
    CultureMech_000059 --> ing0_Deionized_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Deionized_water -.-> CHEBI_15377

Ingredients (1)

# Ingredient Concentration Ontology Notes
1 Deionized water
H2O 18.015 g/mol
1 L CHEBI:15377
water

Preparation

  1. Add 5 ml of each stock solution and make up to 1 L with deionized water.
  2. Autoclave at 15 psi for 15 minutes.

Solutions

NaCl stock solution

Dose: 5.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
NaCl 20 G_PER_L
Prepare with 2.0 g NaCl per 100 ml; add 5 ml/L.

KCl stock solution

Dose: 5.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
KCl 0.8 G_PER_L
Prepare with 0.08 g KCl per 100 ml; add 5 ml/L.

CaCl2 stock solution

Dose: 5.0 ML_PER_L

Stock composition
IngredientConcentrationNotes
CaCl2 1.2 G_PER_L
Prepare with 0.12 g CaCl2 per 100 ml; add 5 ml/L.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-28T00:00:00+00:00 ccap-import Imported from CCAP Culture Collection Source ID: CH, PDF URL: https://www.ccap.ac.uk/wp-content/uploads/MR_CH.pdf
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added placeholder ingredients for missing composition
2026-02-03T11:26:43.481917 quality-tagger-v1.0 Added data quality flag Marked as incomplete_composition due to placeholder ingredients
2026-03-13T06:18:53.049900Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000059
2026-03-17T19:45:04.005056+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "CH" to "ch" for consistent matching
2026-05-16T09:02:56.419451+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2; references.reference_id->reference=2; category->lowercase=1
2026-08-06T07:56:36.020723+00:00 curate_placeholder_composition.py REMOVED_PLACEHOLDER_INGREDIENT Removed the 'See source for composition' placeholder ingredient; the record carries no imported composition and is flagged incomplete (#175).
2026-08-25T00:00:00-07:00 repair_ccap_duplicate_compositions.py SOURCE_CORRECTED_CCAP_DUPLICATE_RECIPE Rebuilt ch from the reviewed CCAP PDF. Preserved stock boundaries and per-vessel quantities; ingredient identities follow the MediaIngredientMech SSSOM.
2026-09-13T00:00:00-07:00 repair_ccap_sources_score10.py NORMALIZED_CCAP_SOURCE_SCORE10 Copied the CCAP source identity from references and the original ccap-import event into the structured sources slot recognized by review scoring.