Overview
- Applications
-
- Algae cultivation
- Phytoplankton culture
- Microalgae research
- Notes
- Full recipe available at https://www.ccap.ac.uk/wp-content/uploads/MR_DM.pdf
Composition
flowchart LR
CultureMech_000063["dm"]
ing0_Na_EDTA["Na EDTA"]
CultureMech_000063 --> ing0_Na_EDTA
CHEBI_64734["CHEBI:64734"]
ing0_Na_EDTA -.-> CHEBI_64734
ing1_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_000063 --> ing1_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing1_MnCl2_x_4_H2O -.-> CHEBI_86368
ing2_Thiamine_HCl["Thiamine HCl"]
CultureMech_000063 --> ing2_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing2_Thiamine_HCl -.-> CHEBI_49105
ing3_Biotin["Biotin"]
CultureMech_000063 --> ing3_Biotin
CHEBI_15956["CHEBI:15956"]
ing3_Biotin -.-> CHEBI_15956
Ingredients (4)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Na EDTA
C10H14N2O8.2Na 336.208 g/mol
|
0.45 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
From CCAP Medium MR_DM.pdf; Curated from CCAP Medium MR_DM.pdf specification |
| 2 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.278 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
From CCAP Medium MR_DM.pdf; Curated from CCAP Medium MR_DM.pdf specification |
| 3 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.008 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
From CCAP Medium MR_DM.pdf; Curated from CCAP Medium MR_DM.pdf specification |
| 4 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.008 G_PER_L |
CHEBI:15956
biotin
|
From CCAP Medium MR_DM.pdf; Curated from CCAP Medium MR_DM.pdf specification |
Preparation
- Bring to 1 L with deionised water.
- Adjust pH to 6.9 with 1 M NaOH or 1 M HCl before autoclaving.
- Autoclave at 15 psi for 15 minutes.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-28T00:00:00+00:00 | ccap-import | Imported from CCAP Culture Collection | Source ID: DM, PDF URL: https://www.ccap.ac.uk/wp-content/uploads/MR_DM.pdf |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added placeholder ingredients for missing composition |
| 2026-02-03T11:26:43.489311 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-03-13T06:18:53.060684Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000063 |
| 2026-03-17T19:45:04.015931+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "DM" to "dm" for consistent matching |
| 2026-04-04T20:18:55.040680 | batch_process_collection_media | EXPANDED_INGREDIENTS | |
| 2026-05-16T08:57:02.266781+00:00 | migrate_data_quality_flags.py | MIGRATED_DATA_QUALITY_FLAGS | dict(4 keys) -> list(3 entries) |
| 2026-05-16T09:02:56.453502+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2; references.reference_id->reference=2; category->lowercase=1 |
| 2026-08-25T00:00:00-07:00 | repair_ccap_instruction_rows.py | REPAIRED_CCAP_INSTRUCTION_ROWS | Repaired preparation text and nested-recipe flattening against CCAP MR_DM.pdf. Moved process statements to preparation_steps and restored source-asserted components or constituent-medium references where applicable. |
| 2026-09-13T00:00:00-07:00 | repair_ccap_sources_score10.py | NORMALIZED_CCAP_SOURCE_SCORE10 | Copied the CCAP source identity from references and the original ccap-import event into the structured sources slot recognized by review scoring. |