Overview
- Applications
-
- Algae cultivation
- Phytoplankton culture
- Microalgae research
- Notes
- Full recipe available at https://www.ccap.ac.uk/wp-content/uploads/MR_E26_Biotin.pdf
Composition
flowchart LR
CultureMech_000064["e26_biotin"]
ing0_KNO["KNO"]
CultureMech_000064 --> ing0_KNO
CHEBI_63043["CHEBI:63043"]
ing0_KNO -.-> CHEBI_63043
ing1_K2HPO4["K2HPO4"]
CultureMech_000064 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_MgSO__7H_O["MgSO .7H O"]
CultureMech_000064 --> ing2_MgSO__7H_O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO__7H_O -.-> CHEBI_31795
ing3_Thiamine_HCl__Vitamin_B__["Thiamine HCl (Vitamin B )"]
CultureMech_000064 --> ing3_Thiamine_HCl__Vitamin_B__
CHEBI_49105["CHEBI:49105"]
ing3_Thiamine_HCl__Vitamin_B__ -.-> CHEBI_49105
Ingredients (4)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KNO
K.NO3 101.102 g/mol
|
0.10 G_PER_L |
CHEBI:63043
potassium nitrate
|
From CCAP Medium MR_E26_Biotin.pdf; Curated from CCAP Medium MR_E26_Biotin.pdf specification |
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.01 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
From CCAP Medium MR_E26_Biotin.pdf; Curated from CCAP Medium MR_E26_Biotin.pdf specification |
| 3 |
MgSO .7H O
7H2O.Mg.O4S 246.473 g/mol
|
0.01 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
From CCAP Medium MR_E26_Biotin.pdf; Curated from CCAP Medium MR_E26_Biotin.pdf specification |
| 4 |
Thiamine HCl (Vitamin B )
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.05 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
From CCAP Medium MR_E26_Biotin.pdf; Curated from CCAP Medium MR_E26_Biotin.pdf specification |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-28T00:00:00+00:00 | ccap-import | Imported from CCAP Culture Collection | Source ID: E26_Biotin, PDF URL: https://www.ccap.ac.uk/wp-content/uploads/MR_E26_Biotin.pdf |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added placeholder ingredients for missing composition |
| 2026-02-03T11:26:43.491159 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-03-13T06:18:53.063389Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000064 |
| 2026-03-17T19:45:04.018530+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "E26 + Biotin" to "e26_biotin" for consistent matching |
| 2026-04-04T12:54:55.109743 | batch_process_collection_media | EXPANDED_INGREDIENTS | |
| 2026-05-16T08:57:02.275997+00:00 | migrate_data_quality_flags.py | MIGRATED_DATA_QUALITY_FLAGS | dict(4 keys) -> list(3 entries) |
| 2026-05-16T09:02:56.465945+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2; references.reference_id->reference=2; category->lowercase=1 |
| 2026-06-10T06:57:11.250848+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-09-13T00:00:00-07:00 | repair_ccap_sources_score10.py | NORMALIZED_CCAP_SOURCE_SCORE10 | Copied the CCAP source identity from references and the original ccap-import event into the structured sources slot recognized by review scoring. |