Overview
- Applications
-
- Algae cultivation
- Phytoplankton culture
- Microalgae research
- Notes
- Full recipe available at https://www.ccap.ac.uk/wp-content/uploads/MR_E26_Biotin_ANT.pdf
Composition
flowchart LR
CultureMech_000065["e26_biotin_ant"]
ing0_KNO["KNO"]
CultureMech_000065 --> ing0_KNO
CHEBI_63043["CHEBI:63043"]
ing0_KNO -.-> CHEBI_63043
ing1_K2HPO4["K2HPO4"]
CultureMech_000065 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_MgSO__7H_O["MgSO .7H O"]
CultureMech_000065 --> ing2_MgSO__7H_O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO__7H_O -.-> CHEBI_31795
ing3_Thiamine_HCl__Vitamin_B__["Thiamine HCl (Vitamin B )"]
CultureMech_000065 --> ing3_Thiamine_HCl__Vitamin_B__
CHEBI_49105["CHEBI:49105"]
ing3_Thiamine_HCl__Vitamin_B__ -.-> CHEBI_49105
ing4_EDTA_Na__2H_O["EDTA.Na .2H O"]
CultureMech_000065 --> ing4_EDTA_Na__2H_O
CHEBI_64734["CHEBI:64734"]
ing4_EDTA_Na__2H_O -.-> CHEBI_64734
ing5_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_000065 --> ing5_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing5_FeCl3_x_6_H2O -.-> CHEBI_86254
ing6_MnSO4_x_4_H2O["MnSO4 x 4 H2O"]
CultureMech_000065 --> ing6_MnSO4_x_4_H2O
CHEBI_86358["CHEBI:86358"]
ing6_MnSO4_x_4_H2O -.-> CHEBI_86358
ing7_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_000065 --> ing7_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing7_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing8_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000065 --> ing8_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing8_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing9_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_000065 --> ing9_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing9_CuSO4_x_5_H2O -.-> CHEBI_31440
ing10_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_000065 --> ing10_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing10_CoSO4_x_7_H2O -.-> CHEBI_91244
ing11_KNO["KNO"]
CultureMech_000065 --> ing11_KNO
ing11_KNO -.-> CHEBI_63043
ing12_NaH2PO4_x_2_H2O["NaH2PO4 x 2 H2O"]
CultureMech_000065 --> ing12_NaH2PO4_x_2_H2O
CHEBI_37585["CHEBI:37585"]
ing12_NaH2PO4_x_2_H2O -.-> CHEBI_37585
ing13_Glycine["Glycine"]
CultureMech_000065 --> ing13_Glycine
CHEBI_15428["CHEBI:15428"]
ing13_Glycine -.-> CHEBI_15428
Ingredients (14)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KNO
K.NO3 101.102 g/mol
|
0.10 G_PER_L |
CHEBI:63043
potassium nitrate
|
From CCAP Medium MR_E26_Biotin_ANT.pdf; Curated from CCAP Medium MR_E26_Biotin_ANT.pdf specification |
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.01 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
From CCAP Medium MR_E26_Biotin_ANT.pdf; Curated from CCAP Medium MR_E26_Biotin_ANT.pdf specification |
| 3 |
MgSO .7H O
7H2O.Mg.O4S 246.473 g/mol
|
0.01 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
From CCAP Medium MR_E26_Biotin_ANT.pdf; Curated from CCAP Medium MR_E26_Biotin_ANT.pdf specification |
| 4 |
Thiamine HCl (Vitamin B )
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.05 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
From CCAP Medium MR_E26_Biotin_ANT.pdf; Curated from CCAP Medium MR_E26_Biotin_ANT.pdf specification |
| 5 |
EDTA.Na .2H O
C10H14N2O8.2Na 336.208 g/mol
|
3.24 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
From CCAP Medium MR_E26_Biotin_ANT.pdf; Curated from CCAP Medium MR_E26_Biotin_ANT.pdf specification |
| 6 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
1.08 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
From CCAP Medium MR_E26_Biotin_ANT.pdf; Curated from CCAP Medium MR_E26_Biotin_ANT.pdf specification |
| 7 |
MnSO4 x 4 H2O
4H2O.Mn.O4S 223.061 g/mol
|
0.450 G_PER_L |
CHEBI:86358
manganese(II) sulfate tetrahydrate
|
From CCAP Medium MR_E26_Biotin_ANT.pdf; Curated from CCAP Medium MR_E26_Biotin_ANT.pdf specification |
| 8 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.230 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
From CCAP Medium MR_E26_Biotin_ANT.pdf; Curated from CCAP Medium MR_E26_Biotin_ANT.pdf specification |
| 9 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.097 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
From CCAP Medium MR_E26_Biotin_ANT.pdf; Curated from CCAP Medium MR_E26_Biotin_ANT.pdf specification |
| 10 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
From CCAP Medium MR_E26_Biotin_ANT.pdf; Curated from CCAP Medium MR_E26_Biotin_ANT.pdf specification |
| 11 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.0056 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
From CCAP Medium MR_E26_Biotin_ANT.pdf; Curated from CCAP Medium MR_E26_Biotin_ANT.pdf specification |
| 12 |
KNO
K.NO3 101.102 g/mol
|
0.05 G_PER_L |
CHEBI:63043
potassium nitrate
|
From CCAP Medium MR_E26_Biotin_ANT.pdf; Curated from CCAP Medium MR_E26_Biotin_ANT.pdf specification |
| 13 |
NaH2PO4 x 2 H2O
H2O4P.Na 119.976 g/mol
|
0.0078 G_PER_L |
CHEBI:37585
sodium dihydrogenphosphate
|
From CCAP Medium MR_E26_Biotin_ANT.pdf; Curated from CCAP Medium MR_E26_Biotin_ANT.pdf specification |
| 14 |
Glycine
C2H5NO2 75.067 g/mol
|
0.3 G_PER_L |
CHEBI:15428
glycine
|
From CCAP Medium MR_E26_Biotin_ANT.pdf; Curated from CCAP Medium MR_E26_Biotin_ANT.pdf specification |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 1 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable). |
| 2026-01-28T00:00:00+00:00 | ccap-import | Imported from CCAP Culture Collection | Source ID: E26_Biotin_ANT, PDF URL: https://www.ccap.ac.uk/wp-content/uploads/MR_E26_Biotin_ANT.pdf |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added placeholder ingredients for missing composition |
| 2026-02-03T11:26:43.493054 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-03-13T06:18:53.066088Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000065 |
| 2026-03-17T19:45:04.021157+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "E26 + Biotin:ANT" to "e26_biotin_ant" for consistent matching |
| 2026-04-04T12:54:55.158038 | batch_process_collection_media | EXPANDED_INGREDIENTS | |
| 2026-05-16T08:57:02.286455+00:00 | migrate_data_quality_flags.py | MIGRATED_DATA_QUALITY_FLAGS | dict(4 keys) -> list(3 entries) |
| 2026-05-16T09:02:56.476230+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2; references.reference_id->reference=2; category->lowercase=1 |
| 2026-06-10T06:57:11.261830+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-09-13T00:00:00-07:00 | repair_ccap_sources_score10.py | NORMALIZED_CCAP_SOURCE_SCORE10 | Copied the CCAP source identity from references and the original ccap-import event into the structured sources slot recognized by review scoring. |