Overview
- Applications
-
- Algae cultivation
- Phytoplankton culture
- Microalgae research
- Notes
- Full recipe available at https://www.ccap.ac.uk/wp-content/uploads/MR_EA.pdf
Composition
flowchart LR
CultureMech_000069["ea"]
ing0_KNO["KNO"]
CultureMech_000069 --> ing0_KNO
CHEBI_63043["CHEBI:63043"]
ing0_KNO -.-> CHEBI_63043
ing1_K2HPO4["K2HPO4"]
CultureMech_000069 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_NaCl["NaCl"]
CultureMech_000069 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_KCl["KCl"]
CultureMech_000069 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_000069 --> ing4_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
ing5_CaSO4_x_2_H2O["CaSO4 x 2 H2O"]
CultureMech_000069 --> ing5_CaSO4_x_2_H2O
CHEBI_32583["CHEBI:32583"]
ing5_CaSO4_x_2_H2O -.-> CHEBI_32583
ing6_Bacto_agar["Bacto agar"]
CultureMech_000069 --> ing6_Bacto_agar
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KNO
K.NO3 101.102 g/mol
|
0.20 G_PER_L |
CHEBI:63043
potassium nitrate
|
From CCAP Medium MR_EA.pdf; Curated from CCAP Medium MR_EA.pdf specification |
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.02 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
From CCAP Medium MR_EA.pdf; Curated from CCAP Medium MR_EA.pdf specification |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
15 G_PER_L |
CHEBI:26710
sodium chloride
|
From CCAP Medium MR_EA.pdf; Curated from CCAP Medium MR_EA.pdf specification |
| 4 |
KCl
Cl.K 74.551 g/mol
|
0.375 G_PER_L |
CHEBI:32588
potassium chloride
|
From CCAP Medium MR_EA.pdf; Curated from CCAP Medium MR_EA.pdf specification |
| 5 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
2.5 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
From CCAP Medium MR_EA.pdf; Curated from CCAP Medium MR_EA.pdf specification |
| 6 |
CaSO4 x 2 H2O
Ca.2H2O.O4S 172.171 g/mol
|
0.5 G_PER_L |
CHEBI:32583
calcium sulfate dihydrate
|
From CCAP Medium MR_EA.pdf; Curated from CCAP Medium MR_EA.pdf specification |
| 7 | Bacto agar | 10 G_PER_L | Ontology mapping not yet available; From CCAP Medium MR_EA.pdf; Curated from CCAP Medium MR_EA.pdf specification |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 1 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable). |
| 2026-01-28T00:00:00+00:00 | ccap-import | Imported from CCAP Culture Collection | Source ID: EA, PDF URL: https://www.ccap.ac.uk/wp-content/uploads/MR_EA.pdf |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added placeholder ingredients for missing composition |
| 2026-02-03T11:26:43.500570 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-03-13T06:18:53.077100Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000069 |
| 2026-03-17T19:45:04.031694+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "EA" to "ea" for consistent matching |
| 2026-04-04T12:54:55.126250 | batch_process_collection_media | EXPANDED_INGREDIENTS | |
| 2026-05-16T08:57:02.318981+00:00 | migrate_data_quality_flags.py | MIGRATED_DATA_QUALITY_FLAGS | dict(4 keys) -> list(3 entries) |
| 2026-05-16T09:02:56.507930+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2; references.reference_id->reference=2; category->lowercase=1 |
| 2026-09-13T00:00:00-07:00 | repair_ccap_sources_score10.py | NORMALIZED_CCAP_SOURCE_SCORE10 | Copied the CCAP source identity from references and the original ccap-import event into the structured sources slot recognized by review scoring. |