Overview
- Applications
-
- Algae cultivation
- Phytoplankton culture
- Microalgae research
- Notes
- Full recipe available at https://www.ccap.ac.uk/wp-content/uploads/MR_EG.pdf
Composition
flowchart LR
CultureMech_000070["eg"]
ing0_Sodium_acetate_trihydrate["Sodium acetate trihydrate"]
CultureMech_000070 --> ing0_Sodium_acetate_trihydrate
CHEBI_32138["CHEBI:32138"]
ing0_Sodium_acetate_trihydrate -.-> CHEBI_32138
ing1_Lab_Lemco_powder["Lab-Lemco powder"]
CultureMech_000070 --> ing1_Lab_Lemco_powder
ing2_Tryptone["Tryptone"]
CultureMech_000070 --> ing2_Tryptone
ing3_Yeast_extract["Yeast extract"]
CultureMech_000070 --> ing3_Yeast_extract
Ingredients (4)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Sodium acetate trihydrate
C2H3O2.3H2O.Na 136.079 g/mol
|
1.0 G_PER_L |
CHEBI:32138
sodium acetate trihydrate
|
Ontology mapping not yet available; From CCAP Medium MR_EG.pdf; Curated from CCAP Medium MR_EG.pdf specification |
| 2 | Lab-Lemco powder | 1.0 G_PER_L | Ontology mapping not yet available; From CCAP Medium MR_EG.pdf; Curated from CCAP Medium MR_EG.pdf specification | |
| 3 | Tryptone | 2.0 G_PER_L |
MICRO:0000182 (source link unavailable)
Tryptone
|
From CCAP Medium MR_EG.pdf; Curated from CCAP Medium MR_EG.pdf specification |
| 4 | Yeast extract | 2.0 G_PER_L |
FOODON:03315426
yeast extract
|
From CCAP Medium MR_EG.pdf; Curated from CCAP Medium MR_EG.pdf specification |
Preparation
- Bring to 1 L with deionised water.
- For an agar formulation, add 15 g/L Bacteriological Agar.
- Autoclave at 15 psi for 15 minutes.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-28T00:00:00+00:00 | ccap-import | Imported from CCAP Culture Collection | Source ID: EG, PDF URL: https://www.ccap.ac.uk/wp-content/uploads/MR_EG.pdf |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added placeholder ingredients for missing composition |
| 2026-02-03T11:26:43.502377 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-03-13T06:18:53.079713Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000070 |
| 2026-03-17T19:45:04.034315+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "EG" to "eg" for consistent matching |
| 2026-04-04T20:18:55.136144 | batch_process_collection_media | EXPANDED_INGREDIENTS | |
| 2026-05-16T08:57:02.325595+00:00 | migrate_data_quality_flags.py | MIGRATED_DATA_QUALITY_FLAGS | dict(4 keys) -> list(3 entries) |
| 2026-05-16T09:02:56.514196+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2; references.reference_id->reference=2; category->lowercase=1 |
| 2026-08-25T00:00:00-07:00 | repair_ccap_instruction_rows.py | REPAIRED_CCAP_INSTRUCTION_ROWS | Repaired preparation text and nested-recipe flattening against CCAP MR_EG.pdf. Moved process statements to preparation_steps and restored source-asserted components or constituent-medium references where applicable. |
| 2026-09-13T00:00:00-07:00 | repair_ccap_sources_score10.py | NORMALIZED_CCAP_SOURCE_SCORE10 | Copied the CCAP source identity from references and the original ccap-import event into the structured sources slot recognized by review scoring. |