Overview
- Applications
-
- Algae cultivation
- Phytoplankton culture
- Microalgae research
- Notes
- Full recipe available at https://www.ccap.ac.uk/wp-content/uploads/MR_L1.pdf
Composition
flowchart LR
CultureMech_000079["l1"]
ing0_Cyanocobalamin__Vitamin_B__["Cyanocobalamin (Vitamin B )"]
CultureMech_000079 --> ing0_Cyanocobalamin__Vitamin_B__
CHEBI_17439["CHEBI:17439"]
ing0_Cyanocobalamin__Vitamin_B__ -.-> CHEBI_17439
ing1_Biotin["Biotin"]
CultureMech_000079 --> ing1_Biotin
CHEBI_15956["CHEBI:15956"]
ing1_Biotin -.-> CHEBI_15956
ing2_Thiamine_HCl__Vitamin_B__["Thiamine HCl (Vitamin B )"]
CultureMech_000079 --> ing2_Thiamine_HCl__Vitamin_B__
CHEBI_49105["CHEBI:49105"]
ing2_Thiamine_HCl__Vitamin_B__ -.-> CHEBI_49105
ing3_Thiamine_HCl__B____["Thiamine HCl (B ) -"]
CultureMech_000079 --> ing3_Thiamine_HCl__B____
ing3_Thiamine_HCl__B____ -.-> CHEBI_49105
ing4_Biotin["Biotin"]
CultureMech_000079 --> ing4_Biotin
ing4_Biotin -.-> CHEBI_15956
ing5_Cyanocobalamin__B__["Cyanocobalamin (B )"]
CultureMech_000079 --> ing5_Cyanocobalamin__B__
ing5_Cyanocobalamin__B__ -.-> CHEBI_17439
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Cyanocobalamin (Vitamin B )
C63H88CoN14O14P 1355.388 g/mol
|
0.0005 G_PER_L |
CHEBI:17439
cyanocob(III)alamin
|
From CCAP Medium MR_L1.pdf; Curated from CCAP Medium MR_L1.pdf specification |
| 2 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.0005 G_PER_L |
CHEBI:15956
biotin
|
From CCAP Medium MR_L1.pdf; Curated from CCAP Medium MR_L1.pdf specification |
| 3 |
Thiamine HCl (Vitamin B )
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.1 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
From CCAP Medium MR_L1.pdf; Curated from CCAP Medium MR_L1.pdf specification |
| 4 |
Thiamine HCl (B ) -
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.1 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
From CCAP Medium MR_L1.pdf; Curated from CCAP Medium MR_L1.pdf specification |
| 5 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.5 G_PER_L |
CHEBI:15956
biotin
|
From CCAP Medium MR_L1.pdf; Curated from CCAP Medium MR_L1.pdf specification |
| 6 |
Cyanocobalamin (B )
C63H88CoN14O14P 1355.388 g/mol
|
0.5 G_PER_L |
CHEBI:17439
cyanocob(III)alamin
|
From CCAP Medium MR_L1.pdf; Curated from CCAP Medium MR_L1.pdf specification |
Preparation
- Bring to 1 L with filtered natural seawater.
- Adjust pH to 8.0 with 1 M NaOH or 1 M HCl before autoclaving.
- For an agar formulation, add 15 g/L Bacteriological Agar.
- Autoclave at 15 psi for 15 minutes.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-28T00:00:00+00:00 | ccap-import | Imported from CCAP Culture Collection | Source ID: L1, PDF URL: https://www.ccap.ac.uk/wp-content/uploads/MR_L1.pdf |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added placeholder ingredients for missing composition |
| 2026-02-03T11:26:43.519658 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-03-13T06:18:53.104053Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000079 |
| 2026-03-17T19:45:04.058647+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "L1" to "l1" for consistent matching |
| 2026-04-04T12:54:54.919880 | batch_process_collection_media | EXPANDED_INGREDIENTS | |
| 2026-05-16T08:57:02.454971+00:00 | migrate_data_quality_flags.py | MIGRATED_DATA_QUALITY_FLAGS | dict(4 keys) -> list(3 entries) |
| 2026-05-16T09:02:56.652264+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2; references.reference_id->reference=2; category->lowercase=1 |
| 2026-08-25T00:00:00-07:00 | repair_ccap_instruction_rows.py | REPAIRED_CCAP_INSTRUCTION_ROWS | Repaired preparation text and nested-recipe flattening against CCAP MR_L1.pdf. Moved process statements to preparation_steps and restored source-asserted components or constituent-medium references where applicable. |
| 2026-09-13T00:00:00-07:00 | repair_ccap_sources_score10.py | NORMALIZED_CCAP_SOURCE_SCORE10 | Copied the CCAP source identity from references and the original ccap-import event into the structured sources slot recognized by review scoring. |