Overview
- Applications
-
- Algae cultivation
- Phytoplankton culture
- Microalgae research
- Notes
- Full recipe available at https://www.ccap.ac.uk/wp-content/uploads/MR_MASM.pdf
Composition
flowchart LR
CultureMech_000082["masm"]
ing0_Na_EDTA["Na EDTA"]
CultureMech_000082 --> ing0_Na_EDTA
CHEBI_64734["CHEBI:64734"]
ing0_Na_EDTA -.-> CHEBI_64734
ing1_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_000082 --> ing1_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing1_FeCl3_x_6_H2O -.-> CHEBI_86254
ing2_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_000082 --> ing2_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing2_MnCl2_x_4_H2O -.-> CHEBI_86368
ing3_ZnCl2["ZnCl2"]
CultureMech_000082 --> ing3_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing3_ZnCl2 -.-> CHEBI_49976
ing4_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_000082 --> ing4_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing4_CoCl2_x_6_H2O -.-> CHEBI_53503
ing5_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000082 --> ing5_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing5_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing6_Tris["Tris"]
CultureMech_000082 --> ing6_Tris
CHEBI_9754["CHEBI:9754"]
ing6_Tris -.-> CHEBI_9754
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Na EDTA
C10H14N2O8.2Na 336.208 g/mol
|
0.75 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
From CCAP Medium MR_MASM.pdf; Curated from CCAP Medium MR_MASM.pdf specification |
| 2 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
0.097 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
From CCAP Medium MR_MASM.pdf; Curated from CCAP Medium MR_MASM.pdf specification |
| 3 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.041 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
From CCAP Medium MR_MASM.pdf; Curated from CCAP Medium MR_MASM.pdf specification |
| 4 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.005 G_PER_L |
CHEBI:49976
zinc dichloride
|
From CCAP Medium MR_MASM.pdf; Curated from CCAP Medium MR_MASM.pdf specification |
| 5 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.002 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
From CCAP Medium MR_MASM.pdf; Curated from CCAP Medium MR_MASM.pdf specification |
| 6 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.004 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
From CCAP Medium MR_MASM.pdf; Curated from CCAP Medium MR_MASM.pdf specification |
| 7 |
Tris
C4H11NO3 121.136 g/mol
|
1.0 G_PER_L |
CHEBI:9754
tris
|
Ontology mapping not yet available; From CCAP Medium MR_MASM.pdf; Curated from CCAP Medium MR_MASM.pdf specification |
Preparation
- Bring to 1 L with deionised water.
- Adjust pH to 8.0 with 1 M NaOH or 1 M HCl before autoclaving.
- Autoclave at 15 psi for 15 minutes.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-28T00:00:00+00:00 | ccap-import | Imported from CCAP Culture Collection | Source ID: MASM, PDF URL: https://www.ccap.ac.uk/wp-content/uploads/MR_MASM.pdf |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added placeholder ingredients for missing composition |
| 2026-02-03T11:26:43.525336 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-03-13T06:18:53.112093Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000082 |
| 2026-03-17T19:45:04.066986+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MASM" to "masm" for consistent matching |
| 2026-04-04T20:18:55.022063 | batch_process_collection_media | EXPANDED_INGREDIENTS | |
| 2026-05-16T08:57:02.478421+00:00 | migrate_data_quality_flags.py | MIGRATED_DATA_QUALITY_FLAGS | dict(4 keys) -> list(3 entries) |
| 2026-05-16T09:02:56.676551+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2; references.reference_id->reference=2; category->lowercase=1 |
| 2026-08-25T00:00:00-07:00 | repair_ccap_instruction_rows.py | REPAIRED_CCAP_INSTRUCTION_ROWS | Repaired preparation text and nested-recipe flattening against CCAP MR_MASM.pdf. Moved process statements to preparation_steps and restored source-asserted components or constituent-medium references where applicable. |
| 2026-09-13T00:00:00-07:00 | repair_ccap_sources_score10.py | NORMALIZED_CCAP_SOURCE_SCORE10 | Copied the CCAP source identity from references and the original ccap-import event into the structured sources slot recognized by review scoring. |