mbbm (CultureMech:000083)

Overview

Applications

Algae cultivation
Phytoplankton culture
Microalgae research

Notes

Full recipe available at https://www.ccap.ac.uk/wp-content/uploads/MR_MBBM.pdf

Composition

flowchart LR
    CultureMech_000083["mbbm"]
    ing0_Bacto_peptone["Bacto peptone"]
    CultureMech_000083 --> ing0_Bacto_peptone
    ing1_Sucrose["Sucrose"]
    CultureMech_000083 --> ing1_Sucrose
    CHEBI_17992["CHEBI:17992"]
    ing1_Sucrose -.-> CHEBI_17992

Ingredients (2)

#	Ingredient	Concentration	Ontology	Notes
1	Bacto peptone	1 G_PER_L	MICRO:0000178 (source link unavailable) peptone	Direct final-medium ingredient.
2	Sucrose C12H22O11 342.297 g/mol	5 G_PER_L	CHEBI:17992 sucrose	Direct final-medium ingredient.

Preparation

Add 1 g Bacto peptone and 5 g sucrose to 1 L 3N-BBM+V.

Solutions

3N-BBM+V

Dose: 1000 ML_PER_L

CultureMech:000039

Source-defined base medium to which peptone and sucrose are added. Source: CCAP Medium MR_MBBM.pdf.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-28T00:00:00+00:00	ccap-import	Imported from CCAP Culture Collection	Source ID: MBBM, PDF URL: https://www.ccap.ac.uk/wp-content/uploads/MR_MBBM.pdf
2026-02-03T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added placeholder ingredients for missing composition
2026-02-03T11:26:43.527309	quality-tagger-v1.0	Added data quality flag	Marked as incomplete_composition due to placeholder ingredients
2026-03-13T06:18:53.114674Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:000083
2026-03-17T19:45:04.069722+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "MBBM" to "mbbm" for consistent matching
2026-04-04T12:54:55.141270	batch_process_collection_media	EXPANDED_INGREDIENTS
2026-05-16T08:57:02.486560+00:00	migrate_data_quality_flags.py	MIGRATED_DATA_QUALITY_FLAGS	dict(4 keys) -> list(3 entries)
2026-05-16T09:02:56.684218+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=2; references.reference_id->reference=2; category->lowercase=1
2026-08-25T00:00:00-07:00	repair_ccap_instruction_rows.py	REPAIRED_CCAP_INSTRUCTION_ROWS	Repaired preparation text and nested-recipe flattening against CCAP MR_MBBM.pdf. Moved process statements to preparation_steps and restored source-asserted components or constituent-medium references where applicable.
2026-09-13T00:00:00-07:00	repair_ccap_sources_score10.py	NORMALIZED_CCAP_SOURCE_SCORE10	Copied the CCAP source identity from references and the original ccap-import event into the structured sources slot recognized by review scoring.