Overview
- Applications
-
- Algae cultivation
- Phytoplankton culture
- Microalgae research
- Notes
- Full recipe available at https://www.ccap.ac.uk/wp-content/uploads/MR_MErds.pdf Composition verified against https://www.ccap.ac.uk/wp-content/uploads/MR_MErds.pdf on 2026-08-25.
Composition
flowchart LR
CultureMech_000089["merds"]
ing0_Filtered_natural_seawater["Filtered natural seawater"]
CultureMech_000089 --> ing0_Filtered_natural_seawater
Ingredients (1)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Filtered natural seawater | 1 L | Make the final medium up to 1 L after adding the stocks. |
Preparation
- Add SES medium and both salt stocks, then make up to 1 L with filtered natural seawater.
- Autoclave at 15 psi for 15 minutes.
- Filter the final medium if precipitation is problematic.
Solutions
SES medium
Dose: 100.00 ML_PER_L
Use the separately defined CCAP SES recipe.
NaNO3 stock solution
Dose: 2.65 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| NaNO3 | 75 G_PER_L |
Prepare with 7.5 g NaNO3 per 100 ml; add 2.65 ml/L.
Na2HPO4.2H2O stock solution
Dose: 0.62 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Na2HPO4.2H2O | 20 G_PER_L |
Prepare with 2.0 g Na2HPO4.2H2O per 100 ml; add 0.62 ml/L.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-28T00:00:00+00:00 | ccap-import | Imported from CCAP Culture Collection | Source ID: MErds, PDF URL: https://www.ccap.ac.uk/wp-content/uploads/MR_MErds.pdf |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added placeholder ingredients for missing composition |
| 2026-02-03T11:26:43.539362 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-03-13T06:18:53.130951Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000089 |
| 2026-03-17T19:45:04.086088+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MErds" to "merds" for consistent matching |
| 2026-05-16T09:02:56.710653+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2; references.reference_id->reference=2; category->lowercase=1 |
| 2026-08-06T07:56:36.036288+00:00 | curate_placeholder_composition.py | REMOVED_PLACEHOLDER_INGREDIENT | Removed the 'See source for composition' placeholder ingredient; the record carries no imported composition and is flagged incomplete (#175). |
| 2026-08-25T00:00:00-07:00 | repair_ccap_duplicate_compositions.py | SOURCE_CORRECTED_CCAP_DUPLICATE_RECIPE | Rebuilt merds from the reviewed CCAP PDF. Preserved stock boundaries and per-vessel quantities; ingredient identities follow the MediaIngredientMech SSSOM. |
| 2026-09-13T00:00:00-07:00 | repair_ccap_sources_score10.py | NORMALIZED_CCAP_SOURCE_SCORE10 | Copied the CCAP source identity from references and the original ccap-import event into the structured sources slot recognized by review scoring. |