mp (CultureMech:000092)

Overview

Applications

Algae cultivation
Phytoplankton culture
Microalgae research

Notes

Full recipe available at https://www.ccap.ac.uk/wp-content/uploads/MR_MP_Modified_Provasoli.pdf

Composition

flowchart LR
    CultureMech_000092["mp"]
    ing0_Fe_NH4_2_SO4_2_6H2O["Fe(NH4)2(SO4)2.6H2O"]
    CultureMech_000092 --> ing0_Fe_NH4_2_SO4_2_6H2O
    ing1_Na_EDTA["Na EDTA"]
    CultureMech_000092 --> ing1_Na_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing1_Na_EDTA -.-> CHEBI_64734
    ing2_Na_EDTA["Na EDTA"]
    CultureMech_000092 --> ing2_Na_EDTA
    ing2_Na_EDTA -.-> CHEBI_64734
    ing3_H3BO3["H3BO3"]
    CultureMech_000092 --> ing3_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing3_H3BO3 -.-> CHEBI_33118
    ing4_MnSO4_H2O["MnSO4.H2O"]
    CultureMech_000092 --> ing4_MnSO4_H2O
    ing5_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000092 --> ing5_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing5_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing6_CoSO__7H2O["CoSO .7H2O"]
    CultureMech_000092 --> ing6_CoSO__7H2O

Ingredients (7)

#	Ingredient	Concentration	Ontology	Notes
1	Fe(NH4)2(SO4)2.6H2O	0.7 G_PER_L		Ontology mapping not yet available; From CCAP Medium MR_MP_Modified_Provasoli.pdf; Curated from CCAP Medium MR_MP_Modified_Provasoli.pdf specification Name repaired (#276): the source string `Fe(NH ) (SO ) .6H O` had lost its subscript digits; restored to `Fe(NH4)2(SO4)2.6H2O` — ferrous ammonium sulfate hexahydrate.
2	Na EDTA C10H14N2O8.2Na 336.208 g/mol	0.6 G_PER_L	CHEBI:64734 EDTA disodium salt (anhydrous)	From CCAP Medium MR_MP_Modified_Provasoli.pdf; Curated from CCAP Medium MR_MP_Modified_Provasoli.pdf specification
3	Na EDTA C10H14N2O8.2Na 336.208 g/mol	1.0 G_PER_L	CHEBI:64734 EDTA disodium salt (anhydrous)	From CCAP Medium MR_MP_Modified_Provasoli.pdf; Curated from CCAP Medium MR_MP_Modified_Provasoli.pdf specification
4	H3BO3 H3BO3 61.833 g/mol	1.12 G_PER_L	CHEBI:33118 boric acid	From CCAP Medium MR_MP_Modified_Provasoli.pdf; Curated from CCAP Medium MR_MP_Modified_Provasoli.pdf specification Name repaired (#276): the source string `H BO` had lost its subscript digits; restored to `H3BO3` — grounded to boric acid (CHEBI:33118).
5	MnSO4.H2O	0.12 G_PER_L		Ontology mapping not yet available; From CCAP Medium MR_MP_Modified_Provasoli.pdf; Curated from CCAP Medium MR_MP_Modified_Provasoli.pdf specification Name repaired (#276): the source string `MnSO .H O` had lost its subscript digits; restored to `MnSO4.H2O` — manganese(II) sulfate monohydrate.
6	ZnSO4 x 7 H2O 7H2O.O4S.Zn 287.558 g/mol	0.022 G_PER_L	CHEBI:32312 zinc sulfate heptahydrate	From CCAP Medium MR_MP_Modified_Provasoli.pdf; Curated from CCAP Medium MR_MP_Modified_Provasoli.pdf specification
7	CoSO .7H2O	0.005 G_PER_L		Ontology mapping not yet available; From CCAP Medium MR_MP_Modified_Provasoli.pdf; Curated from CCAP Medium MR_MP_Modified_Provasoli.pdf specification

Preparation

For an agar formulation, add 15 g/L Bacteriological Agar.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-28T00:00:00+00:00	ccap-import	Imported from CCAP Culture Collection	Source ID: MP_Modified_Provasoli, PDF URL: https://www.ccap.ac.uk/wp-content/uploads/MR_MP_Modified_Provasoli.pdf
2026-02-03T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added placeholder ingredients for missing composition
2026-02-03T11:26:43.545094	quality-tagger-v1.0	Added data quality flag	Marked as incomplete_composition due to placeholder ingredients
2026-03-13T06:18:53.138972Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:000092
2026-03-17T19:45:04.094005+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "MP" to "mp" for consistent matching
2026-04-04T20:18:55.121173	batch_process_collection_media	EXPANDED_INGREDIENTS
2026-05-16T08:57:02.547743+00:00	migrate_data_quality_flags.py	MIGRATED_DATA_QUALITY_FLAGS	dict(4 keys) -> list(3 entries)
2026-05-16T09:02:56.751268+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=2; references.reference_id->reference=2; category->lowercase=1
2026-08-17T17:31:48.453152+00:00	repair_subscript_stripped_names.py	REPAIRED_SUBSCRIPT_STRIPPED_NAME	Restored digits lost from formula ingredient name(s) by an upstream encoding step (#276): Fe(NH ) (SO ) .6H O -> Fe(NH4)2(SO4)2.6H2O; H BO -> H3BO3; MnSO .H O -> MnSO4.H2O. The corrupted original is preserved in each ingredient's notes.
2026-08-25T00:00:00-07:00	repair_ccap_instruction_rows.py	REPAIRED_CCAP_INSTRUCTION_ROWS	Repaired preparation text and nested-recipe flattening against CCAP MR_MP_Modified_Provasoli.pdf. Moved process statements to preparation_steps and restored source-asserted components or constituent-medium references where applicable.
2026-09-13T00:00:00-07:00	repair_ccap_sources_score10.py	NORMALIZED_CCAP_SOURCE_SCORE10	Copied the CCAP source identity from references and the original ccap-import event into the structured sources slot recognized by review scoring.