my75s

CultureMech:000095 algae COMPLEX SOLID_AGAR
Original name: MY75S

Overview

Applications
  • Algae cultivation
  • Phytoplankton culture
  • Microalgae research
Notes
Full recipe available at https://www.ccap.ac.uk/wp-content/uploads/MR_MY75S.pdf

Composition

flowchart LR
    CultureMech_000095["my75s"]
    ing0_Malt_extract["Malt extract"]
    CultureMech_000095 --> ing0_Malt_extract
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_000095 --> ing1_Yeast_extract
    ing2_Bacteriological_agar["Bacteriological agar"]
    CultureMech_000095 --> ing2_Bacteriological_agar
    CHEBI_2509["CHEBI:2509"]
    ing2_Bacteriological_agar -.-> CHEBI_2509
    ing3_Filtered_natural_seawater["Filtered natural seawater"]
    CultureMech_000095 --> ing3_Filtered_natural_seawater
    ing4_Deionised_water["Deionised water"]
    CultureMech_000095 --> ing4_Deionised_water
    CHEBI_15377["CHEBI:15377"]
    ing4_Deionised_water -.-> CHEBI_15377

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 Malt extract 0.1 G_PER_L FOODON:03301056
malt extract
 From CCAP Medium MR_MY75S.pdf; Curated from CCAP Medium MR_MY75S.pdf specification
2 Yeast extract 0.1 G_PER_L FOODON:03315426
yeast extract
 From CCAP Medium MR_MY75S.pdf; Curated from CCAP Medium MR_MY75S.pdf specification
3 Bacteriological agar 15.0 G_PER_L CHEBI:2509
agar
 From CCAP Medium MR_MY75S.pdf; Curated from CCAP Medium MR_MY75S.pdf specification
4 Filtered natural seawater 750 ML_PER_L Source-asserted liquid volume in the final medium.
5 Deionised water
H2O 18.015 g/mol
250 ML_PER_L CHEBI:15377
water
 Source-asserted liquid volume in the final medium.

Preparation

  1. Combine the seawater and deionised water and heat without boiling.
  2. Dissolve the malt extract and yeast extract.
  3. Cool slightly, add 15 g/L Bacteriological Agar, and disperse.
  4. Autoclave at 15 psi for 15 minutes.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-28T00:00:00+00:00 ccap-import Imported from CCAP Culture Collection Source ID: MY75S, PDF URL: https://www.ccap.ac.uk/wp-content/uploads/MR_MY75S.pdf
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added placeholder ingredients for missing composition
2026-02-03T11:26:43.551229 quality-tagger-v1.0 Added data quality flag Marked as incomplete_composition due to placeholder ingredients
2026-03-13T06:18:53.147156Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000095
2026-03-17T19:45:04.101758+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MY75S" to "my75s" for consistent matching
2026-04-04T12:54:54.807263 batch_process_collection_media EXPANDED_INGREDIENTS
2026-05-16T08:57:02.569233+00:00 migrate_data_quality_flags.py MIGRATED_DATA_QUALITY_FLAGS dict(4 keys) -> list(3 entries)
2026-05-16T09:02:56.774102+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2; references.reference_id->reference=2; category->lowercase=1; concentration.unit aliases=1
2026-08-25T00:00:00-07:00 repair_ccap_instruction_rows.py REPAIRED_CCAP_INSTRUCTION_ROWS Repaired preparation text and nested-recipe flattening against CCAP MR_MY75S.pdf. Moved process statements to preparation_steps and restored source-asserted components or constituent-medium references where applicable.
2026-09-13T00:00:00-07:00 repair_ccap_sources_score10.py NORMALIZED_CCAP_SOURCE_SCORE10 Copied the CCAP source identity from references and the original ccap-import event into the structured sources slot recognized by review scoring.