Overview
- Applications
-
- Algae cultivation
- Phytoplankton culture
- Microalgae research
- pH range
- {'min': 7.6, 'max': 7.8}
- Notes
- Full recipe available at https://www.ccap.ac.uk/wp-content/uploads/MR_NSS_low.pdf Composition verified against https://www.ccap.ac.uk/wp-content/uploads/MR_NSS_low.pdf on 2026-08-25.
Composition
flowchart LR
CultureMech_000107["nss_low"]
ing0_Tricine["Tricine"]
CultureMech_000107 --> ing0_Tricine
CHEBI_46760["CHEBI:46760"]
ing0_Tricine -.-> CHEBI_46760
ing1_Filtered_natural_seawater["Filtered natural seawater"]
CultureMech_000107 --> ing1_Filtered_natural_seawater
Ingredients (2)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Tricine | 0.50 G_PER_L |
CHEBI:46760
tricine
|
|
| 2 | Filtered natural seawater | 1 L | Make the medium up to 1 L after adding Tricine and stocks. |
Preparation
- Add Tricine and the three stock solutions at the stated amounts.
- Make up to 1 L with filtered natural seawater.
- Adjust to pH 7.6-7.8 with 1 M NaOH or 1 M HCl.
- Autoclave at 15 psi for 15 minutes.
- Alternative seawater basis: make up to 1 L with deionized water and 33.6 g Instant Ocean sea salts.
Solutions
Extra salts (ASW stock 1)
Dose: 7.50 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| NaNO3 | 15.00 G_PER_L | |
| Na2HPO4 | 0.60 G_PER_L | |
| K2HPO4 | 0.50 G_PER_L |
CCAP NSS Low stock solution 1; stock composition is per litre.
Vitamin solution
Dose: 5.00 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| Biotin | 0.0002 G_PER_L | |
| Calcium pantothenate | 0.02 G_PER_L | |
| Cyanocobalamin | 0.004 G_PER_L | |
| Folic acid | 0.0004 G_PER_L | |
| Inositol | 1.0 G_PER_L | |
| Nicotinic acid | 0.02 G_PER_L | |
| Thiamine HCl | 0.1 G_PER_L | |
| Thymine | 0.6 G_PER_L |
CCAP NSS Low stock solution 2; stock composition is per litre.
Soil Extract 1 (SE1)
Dose: 12.50 ML_PER_L
Use the separately defined CCAP SE1 recipe.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-28T00:00:00+00:00 | ccap-import | Imported from CCAP Culture Collection | Source ID: NSS_low, PDF URL: https://www.ccap.ac.uk/wp-content/uploads/MR_NSS_low.pdf |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added placeholder ingredients for missing composition |
| 2026-02-03T11:26:43.573926 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-03-13T06:18:53.179723Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000107 |
| 2026-03-17T19:45:04.132545+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "NSS Low" to "nss_low" for consistent matching |
| 2026-04-04T12:54:55.239738 | batch_process_collection_media | EXPANDED_INGREDIENTS | |
| 2026-05-16T08:57:02.616741+00:00 | migrate_data_quality_flags.py | MIGRATED_DATA_QUALITY_FLAGS | dict(4 keys) -> list(3 entries) |
| 2026-05-16T09:02:56.823832+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2; references.reference_id->reference=2; category->lowercase=1 |
| 2026-06-10T07:37:34.002999+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-25T00:00:00-07:00 | repair_ccap_missing_compositions.py | RESTORED_CCAP_SOURCE_COMPOSITION | Restored nss from the reviewed authoritative source. Preserved stock boundaries and source alternatives; removed the incomplete flag where present. Ingredient identities follow the MediaIngredientMech SSSOM. |
| 2026-09-13T00:00:00-07:00 | repair_ccap_sources_score10.py | NORMALIZED_CCAP_SOURCE_SCORE10 | Copied the CCAP source identity from references and the original ccap-import event into the structured sources slot recognized by review scoring. |