Overview
- Applications
-
- Algae cultivation
- Phytoplankton culture
- Microalgae research
- Notes
- Full recipe available at https://www.ccap.ac.uk/wp-content/uploads/MR_PE.pdf
Composition
flowchart LR
CultureMech_000110["pe"]
ing0_Sodium_nitrate["Sodium nitrate"]
CultureMech_000110 --> ing0_Sodium_nitrate
CHEBI_63005["CHEBI:63005"]
ing0_Sodium_nitrate -.-> CHEBI_63005
ing1_Disodium_hydrogen_phosphate_dodecahydrate["Disodium hydrogen phosphate dodecahydrate"]
CultureMech_000110 --> ing1_Disodium_hydrogen_phosphate_dodecahydrate
CHEBI_86416["CHEBI:86416"]
ing1_Disodium_hydrogen_phosphate_dodecahydrate -.-> CHEBI_86416
ing2_Natural_seawater__filtered__95__strength_["Natural seawater (filtered, 95% strength)"]
CultureMech_000110 --> ing2_Natural_seawater__filtered__95__strength_
ing3_Soil_extract__SE1_["Soil extract (SE1)"]
CultureMech_000110 --> ing3_Soil_extract__SE1_
ing4_Salt_solution_stock["Salt solution stock"]
CultureMech_000110 --> ing4_Salt_solution_stock
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Sodium nitrate
NO3.Na 84.994 g/mol
|
0.2 G_PER_L |
CHEBI:63005
sodium nitrate
|
From salt solution stock (200 g/L stock, 1 mL/L final); nitrogen source |
| 2 |
Disodium hydrogen phosphate dodecahydrate
C48H90NO8P 840.221 g/mol
|
0.02 G_PER_L |
CHEBI:86416
1-eicosanoyl-2-[(11Z,14Z,17Z)-eicosatrienoyl]-sn-glycero-3-phosphocholine
|
From salt solution stock (20 g/L stock, 1 mL/L final); phosphate source and buffer |
| 3 | Natural seawater (filtered, 95% strength) | 950 ML_PER_L |
ENVO:00002149
sea water
|
Filtered natural seawater diluted to 95% with distilled water; provides minerals and salts |
| 4 | Soil extract (SE1) | 50 ML_PER_L | Soil extract preparation SE1 (see CCAP protocols); provides vitamins, trace elements, and growth factors | |
| 5 | Salt solution stock | 1 ML_PER_L | Contains NaNO3 and Na2HPO4·12H2O (see above for individual concentrations) |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 1 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable). |
| 2026-01-28T00:00:00+00:00 | ccap-import | Imported from CCAP Culture Collection | Source ID: PE, PDF URL: https://www.ccap.ac.uk/wp-content/uploads/MR_PE.pdf |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added placeholder ingredients for missing composition |
| 2026-02-03T11:26:43.579462 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-03-13T06:18:53.188028Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000110 |
| 2026-03-17T19:45:04.140386+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PE" to "pe" for consistent matching |
| 2026-04-05T17:18:31.712351 | expand_pe_medium | EXPANDED_MANUAL | PDF was accessible but failed automated table extraction; composition manually extracted from text |
| 2026-05-16T08:57:02.638014+00:00 | migrate_data_quality_flags.py | MIGRATED_DATA_QUALITY_FLAGS | dict(4 keys) -> list(3 entries) |
| 2026-05-16T09:02:56.845913+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2; references.reference_id->reference=2; category->lowercase=1 |
| 2026-09-13T00:00:00-07:00 | repair_ccap_sources_score10.py | NORMALIZED_CCAP_SOURCE_SCORE10 | Copied the CCAP source identity from references and the original ccap-import event into the structured sources slot recognized by review scoring. |