r75s

CultureMech:000118 algae COMPLEX LIQUID
Original name: R75S

Overview

Applications
  • Algae cultivation
  • Phytoplankton culture
  • Microalgae research
Notes
CCAP prepares R75S from 750 mL natural filtered seawater, 250 mL deionized water, and 1 g dried milled rye grass per liter.

Composition

flowchart LR
    CultureMech_000118["r75s"]
    ing0_Natural_filtered_seawater["Natural filtered seawater"]
    CultureMech_000118 --> ing0_Natural_filtered_seawater
    ing1_Deionized_water["Deionized water"]
    CultureMech_000118 --> ing1_Deionized_water
    CHEBI_15377["CHEBI:15377"]
    ing1_Deionized_water -.-> CHEBI_15377
    ing2_Dried_milled_rye_grass["Dried milled rye grass"]
    CultureMech_000118 --> ing2_Dried_milled_rye_grass

Ingredients (3)

# Ingredient Concentration Ontology Notes
1 Natural filtered seawater 750 ML_PER_L ENVO:00002149
sea water
 CCAP lists 750 mL Natural filtered seawater per liter.
2 Deionized water
H2O 18.015 g/mol
250 ML_PER_L CHEBI:15377
water
 CCAP lists 250 mL Deionized water per liter.
3 Dried milled rye grass 1 G_PER_L CCAP lists 1 g/L dried milled rye grass.

Preparation

  1. Prepare 75% seawater from natural filtered seawater and deionized water.
  2. Bring the 75% seawater to the boil, add the powdered rye grass, and boil for 5 minutes.
  3. Cool and restore the final volume to 1 L with deionized water.
  4. Filter through Whatman GF/C paper.
  5. Autoclave at 15 psi for 15 minutes.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-28T00:00:00+00:00 ccap-import Imported from CCAP Culture Collection Source ID: R75S, PDF URL: https://www.ccap.ac.uk/wp-content/uploads/MR_R75S.pdf
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added placeholder ingredients for missing composition
2026-02-03T11:26:43.594416 quality-tagger-v1.0 Added data quality flag Marked as incomplete_composition due to placeholder ingredients
2026-03-13T06:18:53.209826Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000118
2026-03-17T19:45:04.160902+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "R75S" to "r75s" for consistent matching
2026-04-04T12:54:55.071923 batch_process_collection_media EXPANDED_INGREDIENTS
2026-05-16T08:57:02.681894+00:00 migrate_data_quality_flags.py MIGRATED_DATA_QUALITY_FLAGS dict(4 keys) -> list(2 entries)
2026-05-16T09:02:56.894784+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2; references.reference_id->reference=2; category->lowercase=1; concentration.unit aliases=1
2026-09-06T00:00:00-07:00 repair_algae_score45_blends_soils.py RESOLVED_SCORE45_ALGAE_GRAPH CCAP prepares R75S from 750 mL natural filtered seawater, 250 mL deionized water, and 1 g dried milled rye grass per liter.
2026-09-06T00:00:00-07:00 repair_algae_score45_blends_soils.py LINKED_SCORE45_ALGAE_CHILDREN Added or refreshed reciprocal parent-child link for algae/r75s_nsw.yaml.
2026-09-13T00:00:00-07:00 repair_ccap_sources_score10.py NORMALIZED_CCAP_SOURCE_SCORE10 Copied the CCAP source identity from references and the original ccap-import event into the structured sources slot recognized by review scoring.