Overview
- Applications
-
- Algae cultivation
- Phytoplankton culture
- Microalgae research
- pH
- 7.1
- Notes
- CCAP prepares SE2 by twice autoclaving dried calcareous garden loam with twice its volume of deionized water, settling it for 2-3 weeks, decanting the supernatant, and autoclaving the final extract.
Composition
flowchart LR
CultureMech_000129["se2"]
ing0_Prepared_dried_calcareous_garden_loam["Prepared dried calcareous garden loam"]
CultureMech_000129 --> ing0_Prepared_dried_calcareous_garden_loam
ing1_Deionized_water["Deionized water"]
CultureMech_000129 --> ing1_Deionized_water
CHEBI_15377["CHEBI:15377"]
ing1_Deionized_water -.-> CHEBI_15377
Ingredients (2)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Prepared dried calcareous garden loam | variable VARIABLE |
ENVO:00001998
soil
|
CCAP specifies air-dried and sieved calcareous garden loam. |
| 2 |
Deionized water
H2O 18.015 g/mol
|
2 volumes per 1 volume dried soil VARIABLE |
CHEBI:15377
water
|
CCAP mixes dried soil with twice its volume of deionized water. |
Preparation
- Air-dry calcareous garden loam, remove debris, and sieve it.
- Combine dried soil with twice its volume of deionized water.
- Autoclave at 15 psi and 126 C for 20 minutes, cool, and repeat once.
- Seal and leave undisturbed for 2-3 weeks to settle.
- Aseptically decant the supernatant into sterile containers.
- Autoclave the final soil extract at 15 psi and 121 C for 15 minutes.
- Cool, seal, and store refrigerated.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-28T00:00:00+00:00 | ccap-import | Imported from CCAP Culture Collection | Source ID: SE2_Freshwater, PDF URL: https://www.ccap.ac.uk/wp-content/uploads/MR_SE2_Freshwater.pdf |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added placeholder ingredients for missing composition |
| 2026-02-03T11:26:43.615200 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-03-13T06:18:53.239369Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000129 |
| 2026-03-17T19:45:04.189081+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SE2" to "se2" for consistent matching |
| 2026-05-16T09:02:56.970335+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2; references.reference_id->reference=2; category->lowercase=1 |
| 2026-08-06T07:56:36.054224+00:00 | curate_placeholder_composition.py | REMOVED_PLACEHOLDER_INGREDIENT | Removed the 'See source for composition' placeholder ingredient; the record carries no imported composition and is flagged incomplete (#175). |
| 2026-08-25T00:00:00-07:00 | repair_ccap_duplicate_compositions.py | SOURCE_CORRECTED_CCAP_DUPLICATE_RECIPE | Rebuilt se2 from the reviewed CCAP PDF. Preserved stock boundaries and per-vessel quantities; ingredient identities follow the MediaIngredientMech SSSOM. |
| 2026-09-06T00:00:00-07:00 | repair_algae_score45_blends_soils.py | RESOLVED_SCORE45_ALGAE_GRAPH | CCAP prepares SE2 by twice autoclaving dried calcareous garden loam with twice its volume of deionized water, settling it for 2-3 weeks, decanting the supernatant, and autoclaving the final extract. |
| 2026-09-13T00:00:00-07:00 | repair_ccap_sources_score10.py | NORMALIZED_CCAP_SOURCE_SCORE10 | Copied the CCAP source identity from references and the original ccap-import event into the structured sources slot recognized by review scoring. |