Overview
- Applications
-
- Algae cultivation
- Phytoplankton culture
- Microalgae research
- Notes
- Full recipe available at https://www.ccap.ac.uk/wp-content/uploads/MR_SES.pdf Composition verified against https://www.ccap.ac.uk/wp-content/uploads/MR_SES.pdf on 2026-08-25.
Composition
flowchart LR
CultureMech_000130["ses"]
ing0_Deionized_water["Deionized water"]
CultureMech_000130 --> ing0_Deionized_water
CHEBI_15377["CHEBI:15377"]
ing0_Deionized_water -.-> CHEBI_15377
Ingredients (1)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Deionized water
H2O 18.015 g/mol
|
1 L |
CHEBI:15377
water
|
Preparation
- Add the four stock solutions and make up to 1 L with deionized water.
- Autoclave at 15 psi for 15 minutes.
Solutions
K2HPO4 stock solution
Dose: 20.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| K2HPO4 | 1.0 G_PER_L |
CCAP SES stock solution 1; add 20 ml/L.
MgSO4.7H2O stock solution
Dose: 20.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| MgSO4.7H2O | 1.0 G_PER_L |
CCAP SES stock solution 2; add 20 ml/L.
KNO3 stock solution
Dose: 20.0 ML_PER_L
| Ingredient | Concentration | Notes |
|---|---|---|
| KNO3 | 10.0 G_PER_L |
CCAP SES stock solution 3; add 20 ml/L.
Soil Extract 2 (SE2)
Dose: 100.0 ML_PER_L
Use the separately defined CCAP SE2 recipe.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-28T00:00:00+00:00 | ccap-import | Imported from CCAP Culture Collection | Source ID: SES, PDF URL: https://www.ccap.ac.uk/wp-content/uploads/MR_SES.pdf |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added placeholder ingredients for missing composition |
| 2026-02-03T11:26:43.617029 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-03-13T06:18:53.242035Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000130 |
| 2026-03-17T19:45:04.191568+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SES" to "ses" for consistent matching |
| 2026-05-16T09:02:56.976151+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2; references.reference_id->reference=2; category->lowercase=1 |
| 2026-08-06T07:56:36.057347+00:00 | curate_placeholder_composition.py | REMOVED_PLACEHOLDER_INGREDIENT | Removed the 'See source for composition' placeholder ingredient; the record carries no imported composition and is flagged incomplete (#175). |
| 2026-08-25T00:00:00-07:00 | repair_ccap_duplicate_compositions.py | SOURCE_CORRECTED_CCAP_DUPLICATE_RECIPE | Rebuilt ses from the reviewed CCAP PDF. Preserved stock boundaries and per-vessel quantities; ingredient identities follow the MediaIngredientMech SSSOM. |
| 2026-09-13T00:00:00-07:00 | repair_ccap_sources_score10.py | NORMALIZED_CCAP_SOURCE_SCORE10 | Copied the CCAP source identity from references and the original ccap-import event into the structured sources slot recognized by review scoring. |