s_w_ca

CultureMech:000137 algae COMPLEX BIPHASIC
Original name: S/W + Ca

Overview

Applications
  • Algae cultivation
  • Phytoplankton culture
  • Microalgae research
pH range
{'min': 7.0, 'max': 8.0}
Notes
Full recipe available at https://www.ccap.ac.uk/wp-content/uploads/MR_SW_Ca.pdf Composition verified against https://www.ccap.ac.uk/wp-content/uploads/MR_SW_Ca.pdf on 2026-08-25.

Composition

flowchart LR
    CultureMech_000137["s_w_ca"]
    ing0_Calcium_carbonate["Calcium carbonate"]
    CultureMech_000137 --> ing0_Calcium_carbonate
    CHEBI_3311["CHEBI:3311"]
    ing0_Calcium_carbonate -.-> CHEBI_3311
    ing1_Air_dried_sieved_calcareous_soil["Air-dried sieved calcareous soil"]
    CultureMech_000137 --> ing1_Air_dried_sieved_calcareous_soil
    ing2_Deionized_water["Deionized water"]
    CultureMech_000137 --> ing2_Deionized_water

Ingredients (3)

# Ingredient Concentration Ontology Notes
1 Calcium carbonate
CO3.Ca 100.086 g/mol
0.01 g per vessel VARIABLE CHEBI:3311
calcium carbonate
 Place in the base of each vessel before adding soil and water.
2 Air-dried sieved calcareous soil variable VARIABLE Add a layer approximately 1 cm deep to each vessel.
3 Deionized water variable VARIABLE Add carefully to a depth of 7-10 cm above the soil layer.

Preparation

  1. Place approximately 0.01 g calcium carbonate in the base of each vessel.
  2. Place a 1 cm layer of air-dried, sieved calcareous soil in each vessel.
  3. Optionally add one grain for organisms that benefit from it.
  4. Add deionized water carefully to a depth of 7-10 cm, then plug or cover.
  5. Autoclave at 15 psi for 15 minutes on each of two consecutive days.
  6. Let stand for at least one further day before inoculation.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-28T00:00:00+00:00 ccap-import Imported from CCAP Culture Collection Source ID: SW_Ca, PDF URL: https://www.ccap.ac.uk/wp-content/uploads/MR_SW_Ca.pdf
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added placeholder ingredients for missing composition
2026-02-03T11:26:43.630074 quality-tagger-v1.0 Added data quality flag Marked as incomplete_composition due to placeholder ingredients
2026-03-13T06:18:53.261233Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000137
2026-03-17T19:45:04.209151+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "S/W + Ca" to "s_w_ca" for consistent matching
2026-05-16T09:02:56.956235+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2; references.reference_id->reference=2; category->lowercase=1
2026-08-06T07:56:36.051256+00:00 curate_placeholder_composition.py REMOVED_PLACEHOLDER_INGREDIENT Removed the 'See source for composition' placeholder ingredient; the record carries no imported composition and is flagged incomplete (#175).
2026-08-25T00:00:00-07:00 repair_ccap_duplicate_compositions.py SOURCE_CORRECTED_CCAP_DUPLICATE_RECIPE Rebuilt sw_ca from the reviewed CCAP PDF. Preserved stock boundaries and per-vessel quantities; ingredient identities follow the MediaIngredientMech SSSOM.
2026-09-10T00:00:00-07:00 repair_ccap_sw_flags_score20.py MARKED_CCAP_SW_SPARSE_SCORE20_GRAPH Marked reviewed CCAP S/W soil-water components as intentionally curated with unmapped bulk material identities.
2026-09-13T00:00:00-07:00 repair_ccap_sources_score10.py NORMALIZED_CCAP_SOURCE_SCORE10 Copied the CCAP source identity from references and the original ccap-import event into the structured sources slot recognized by review scoring.