s_w

CultureMech:000138 algae COMPLEX BIPHASIC
Original name: S/W

Overview

Applications
  • Algae cultivation
  • Phytoplankton culture
  • Microalgae research
Notes
CCAP prepares S/W as a biphasic soil/water medium with a 1 cm layer of air-dried sieved calcareous soil and 7-10 cm deionized water.

Composition

flowchart LR
    CultureMech_000138["s_w"]
    ing0_Air_dried_sieved_calcareous_soil["Air-dried sieved calcareous soil"]
    CultureMech_000138 --> ing0_Air_dried_sieved_calcareous_soil
    ing1_Deionized_water["Deionized water"]
    CultureMech_000138 --> ing1_Deionized_water
    CHEBI_15377["CHEBI:15377"]
    ing1_Deionized_water -.-> CHEBI_15377

Ingredients (2)

# Ingredient Concentration Ontology Notes
1 Air-dried sieved calcareous soil variable VARIABLE ENVO:00001998
soil
 CCAP adds a 1 cm layer of air-dried, sieved calcareous soil.
2 Deionized water
H2O 18.015 g/mol
7-10 cm above the soil layer VARIABLE CHEBI:15377
water
 CCAP adds deionized water to a depth of 7-10 cm above the soil.

Preparation

  1. Place a 1 cm layer of air-dried, sieved calcareous soil in the vessel.
  2. Optionally add a grain for organisms that benefit from it.
  3. Carefully add deionized water to a depth of 7-10 cm above the soil.
  4. Autoclave at 15 psi for 15 minutes on each of two consecutive days.
  5. Allow to stand for at least one further day before inoculation.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-28T00:00:00+00:00 ccap-import Imported from CCAP Culture Collection Source ID: SW, PDF URL: https://www.ccap.ac.uk/wp-content/uploads/MR_SW.pdf
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added placeholder ingredients for missing composition
2026-02-03T11:26:43.631918 quality-tagger-v1.0 Added data quality flag Marked as incomplete_composition due to placeholder ingredients
2026-03-13T06:18:53.263949Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000138
2026-03-17T19:45:04.211655+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "S/W" to "s_w" for consistent matching
2026-05-16T09:02:56.950271+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2; references.reference_id->reference=2; category->lowercase=1
2026-08-06T07:56:36.048521+00:00 curate_placeholder_composition.py REMOVED_PLACEHOLDER_INGREDIENT Removed the 'See source for composition' placeholder ingredient; the record carries no imported composition and is flagged incomplete (#175).
2026-08-25T00:00:00-07:00 repair_ccap_duplicate_compositions.py SOURCE_CORRECTED_CCAP_DUPLICATE_RECIPE Rebuilt sw from the reviewed CCAP PDF. Preserved stock boundaries and per-vessel quantities; ingredient identities follow the MediaIngredientMech SSSOM.
2026-09-06T00:00:00-07:00 repair_algae_score45_blends_soils.py RESOLVED_SCORE45_ALGAE_GRAPH CCAP prepares S/W as a biphasic soil/water medium with a 1 cm layer of air-dried sieved calcareous soil and 7-10 cm deionized water.
2026-09-13T00:00:00-07:00 repair_ccap_sources_score10.py NORMALIZED_CCAP_SOURCE_SCORE10 Copied the CCAP source identity from references and the original ccap-import event into the structured sources slot recognized by review scoring.