Overview
- Applications
-
- Algae cultivation
- Phytoplankton culture
- Microalgae research
- Notes
- Full recipe available at https://www.ccap.ac.uk/wp-content/uploads/MR_UM.pdf
Composition
flowchart LR
CultureMech_000141["um"]
ing0_NaNO["NaNO"]
CultureMech_000141 --> ing0_NaNO
CHEBI_34545["CHEBI:34545"]
ing0_NaNO -.-> CHEBI_34545
ing1_Na2HPO4["Na2HPO4"]
CultureMech_000141 --> ing1_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing1_Na2HPO4 -.-> CHEBI_34683
ing2_K2HPO4["K2HPO4"]
CultureMech_000141 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_Tricine["Tricine"]
CultureMech_000141 --> ing3_Tricine
CHEBI_46760["CHEBI:46760"]
ing3_Tricine -.-> CHEBI_46760
ing4_Complan["Complan"]
CultureMech_000141 --> ing4_Complan
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaNO
C23H28ClN5O3 457.962 g/mol
|
15.00 G_PER_L |
CHEBI:34545
Azimilide
|
From CCAP Medium MR_UM.pdf; Curated from CCAP Medium MR_UM.pdf specification |
| 2 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
0.60 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
From CCAP Medium MR_UM.pdf; Curated from CCAP Medium MR_UM.pdf specification |
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.50 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
From CCAP Medium MR_UM.pdf; Curated from CCAP Medium MR_UM.pdf specification |
| 4 | Tricine | 0.5 G_PER_L |
CHEBI:46760
tricine
|
From CCAP Medium MR_UM.pdf; Curated from CCAP Medium MR_UM.pdf specification |
| 5 | Complan | 10.0 G_PER_L | Ontology mapping not yet available; From CCAP Medium MR_UM.pdf; Curated from CCAP Medium MR_UM.pdf specification |
Preparation
- Bring to 1 L with deionised water.
- Adjust pH to 7.6-7.8 with 1 M NaOH or 1 M HCl before autoclaving.
- Autoclave at 15 psi for 15 minutes.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-28T00:00:00+00:00 | ccap-import | Imported from CCAP Culture Collection | Source ID: UM, PDF URL: https://www.ccap.ac.uk/wp-content/uploads/MR_UM.pdf |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added placeholder ingredients for missing composition |
| 2026-02-03T11:26:43.637543 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-03-13T06:18:53.272458Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000141 |
| 2026-03-17T19:45:04.219760+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "UM" to "um" for consistent matching |
| 2026-04-04T12:54:54.875494 | batch_process_collection_media | EXPANDED_INGREDIENTS | |
| 2026-05-16T08:57:02.852435+00:00 | migrate_data_quality_flags.py | MIGRATED_DATA_QUALITY_FLAGS | dict(4 keys) -> list(3 entries) |
| 2026-05-16T09:02:57.084497+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2; references.reference_id->reference=2; category->lowercase=1 |
| 2026-08-25T00:00:00-07:00 | repair_ccap_instruction_rows.py | REPAIRED_CCAP_INSTRUCTION_ROWS | Repaired preparation text and nested-recipe flattening against CCAP MR_UM.pdf. Moved process statements to preparation_steps and restored source-asserted components or constituent-medium references where applicable. |
| 2026-09-13T00:00:00-07:00 | repair_ccap_sources_score10.py | NORMALIZED_CCAP_SOURCE_SCORE10 | Copied the CCAP source identity from references and the original ccap-import event into the structured sources slot recognized by review scoring. |