Overview
- Applications
-
- Algae cultivation
- Phytoplankton culture
- Microalgae research
- Notes
- Full recipe available at https://www.ccap.ac.uk/wp-content/uploads/MR_ZM10.pdf
Composition
flowchart LR
CultureMech_000146["zm_10"]
ing0_Na_EDTA["Na EDTA"]
CultureMech_000146 --> ing0_Na_EDTA
CHEBI_64734["CHEBI:64734"]
ing0_Na_EDTA -.-> CHEBI_64734
ing1_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_000146 --> ing1_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing1_FeCl3_x_6_H2O -.-> CHEBI_86254
ing2_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_000146 --> ing2_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing2_CuSO4_x_5_H2O -.-> CHEBI_31440
ing3_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_000146 --> ing3_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing3_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing4_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_000146 --> ing4_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing4_CoCl2_x_6_H2O -.-> CHEBI_53503
ing5_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_000146 --> ing5_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing5_MnCl2_x_4_H2O -.-> CHEBI_86368
ing6_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_000146 --> ing6_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing6_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing7_Cyanocobalamin["Cyanocobalamin"]
CultureMech_000146 --> ing7_Cyanocobalamin
CHEBI_17439["CHEBI:17439"]
ing7_Cyanocobalamin -.-> CHEBI_17439
ing8_Biotin["Biotin"]
CultureMech_000146 --> ing8_Biotin
CHEBI_15956["CHEBI:15956"]
ing8_Biotin -.-> CHEBI_15956
ing9_Thiamine_HCl["Thiamine HCl"]
CultureMech_000146 --> ing9_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing9_Thiamine_HCl -.-> CHEBI_49105
ing10_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_000146 --> ing10_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing10_Calcium_pantothenate -.-> CHEBI_31345
ing11_Folic_acid["Folic acid"]
CultureMech_000146 --> ing11_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing11_Folic_acid -.-> CHEBI_27470
ing12_Riboflavin["Riboflavin"]
CultureMech_000146 --> ing12_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing12_Riboflavin -.-> CHEBI_17015
ing13_Nicotinamide__Niacinamide_["Nicotinamide (Niacinamide)"]
CultureMech_000146 --> ing13_Nicotinamide__Niacinamide_
ing14_Proteose_peptone["Proteose peptone"]
CultureMech_000146 --> ing14_Proteose_peptone
ing15_Yeast_extract["Yeast extract"]
CultureMech_000146 --> ing15_Yeast_extract
ing16_Agar__Molecular_Genetics_["Agar (Molecular Genetics)"]
CultureMech_000146 --> ing16_Agar__Molecular_Genetics_
ing17_Filtered_natural_seawater["Filtered natural seawater"]
CultureMech_000146 --> ing17_Filtered_natural_seawater
ing18_Deionised_water["Deionised water"]
CultureMech_000146 --> ing18_Deionised_water
CHEBI_15377["CHEBI:15377"]
ing18_Deionised_water -.-> CHEBI_15377
Ingredients (19)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Na EDTA
C10H14N2O8.2Na 336.208 g/mol
|
8.32 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
From CCAP Medium MR_ZM10.pdf; Curated from CCAP Medium MR_ZM10.pdf specification |
| 2 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
6.30 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
From CCAP Medium MR_ZM10.pdf; Curated from CCAP Medium MR_ZM10.pdf specification |
| 3 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.02 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
From CCAP Medium MR_ZM10.pdf; Curated from CCAP Medium MR_ZM10.pdf specification |
| 4 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.044 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
From CCAP Medium MR_ZM10.pdf; Curated from CCAP Medium MR_ZM10.pdf specification |
| 5 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.02 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
From CCAP Medium MR_ZM10.pdf; Curated from CCAP Medium MR_ZM10.pdf specification |
| 6 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.36 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
From CCAP Medium MR_ZM10.pdf; Curated from CCAP Medium MR_ZM10.pdf specification |
| 7 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.012 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
From CCAP Medium MR_ZM10.pdf; Curated from CCAP Medium MR_ZM10.pdf specification |
| 8 |
Cyanocobalamin
C63H88CoN14O14P 1355.388 g/mol
|
0.001 G_PER_L |
CHEBI:17439
cyanocob(III)alamin
|
From CCAP Medium MR_ZM10.pdf; Curated from CCAP Medium MR_ZM10.pdf specification |
| 9 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.02 G_PER_L |
CHEBI:15956
biotin
|
From CCAP Medium MR_ZM10.pdf; Curated from CCAP Medium MR_ZM10.pdf specification |
| 10 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.05 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
From CCAP Medium MR_ZM10.pdf; Curated from CCAP Medium MR_ZM10.pdf specification |
| 11 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.05 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
From CCAP Medium MR_ZM10.pdf; Curated from CCAP Medium MR_ZM10.pdf specification |
| 12 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
From CCAP Medium MR_ZM10.pdf; Curated from CCAP Medium MR_ZM10.pdf specification |
| 13 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.05 G_PER_L |
CHEBI:17015
riboflavin
|
From CCAP Medium MR_ZM10.pdf; Curated from CCAP Medium MR_ZM10.pdf specification |
| 14 | Nicotinamide (Niacinamide) | 0.05 G_PER_L | Ontology mapping not yet available; From CCAP Medium MR_ZM10.pdf; Curated from CCAP Medium MR_ZM10.pdf specification | |
| 15 | Proteose peptone | 0.5 G_PER_L |
MICRO:0000180 (source link unavailable)
Proteose peptone
|
From CCAP Medium MR_ZM10.pdf; Curated from CCAP Medium MR_ZM10.pdf specification |
| 16 | Yeast extract | 0.1 G_PER_L |
FOODON:03315426
yeast extract
|
From CCAP Medium MR_ZM10.pdf; Curated from CCAP Medium MR_ZM10.pdf specification |
| 17 | Agar (Molecular Genetics) | 15.0 G_PER_L | Ontology mapping not yet available; From CCAP Medium MR_ZM10.pdf; Curated from CCAP Medium MR_ZM10.pdf specification | |
| 18 | Filtered natural seawater | 750 ML_PER_L | Source-asserted 75% volume fraction in the final medium. | |
| 19 |
Deionised water
H2O 18.015 g/mol
|
250 ML_PER_L |
CHEBI:15377
water
|
Source-asserted 25% volume fraction in the final medium. |
Preparation
- Bring to 1 L with 75% filtered natural seawater and 25% deionised water.
- Autoclave at 15 psi for 15 minutes.
- Cool to approximately 55 deg C before adding sterile marine supplement stock.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-28T00:00:00+00:00 | ccap-import | Imported from CCAP Culture Collection | Source ID: ZM10, PDF URL: https://www.ccap.ac.uk/wp-content/uploads/MR_ZM10.pdf |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added placeholder ingredients for missing composition |
| 2026-02-03T11:26:43.646891 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-03-13T06:18:53.286042Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000146 |
| 2026-03-17T19:45:04.232792+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ZM/10" to "zm_10" for consistent matching |
| 2026-04-04T20:18:55.054066 | batch_process_collection_media | EXPANDED_INGREDIENTS | |
| 2026-05-16T08:57:02.914496+00:00 | migrate_data_quality_flags.py | MIGRATED_DATA_QUALITY_FLAGS | dict(4 keys) -> list(3 entries) |
| 2026-05-16T09:02:57.161112+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2; references.reference_id->reference=2; category->lowercase=1; concentration.unit aliases=1 |
| 2026-06-10T07:37:34.015098+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-25T00:00:00-07:00 | repair_ccap_instruction_rows.py | REPAIRED_CCAP_INSTRUCTION_ROWS | Repaired preparation text and nested-recipe flattening against CCAP MR_ZM10.pdf. Moved process statements to preparation_steps and restored source-asserted components or constituent-medium references where applicable. |
| 2026-09-13T00:00:00-07:00 | repair_ccap_sources_score10.py | NORMALIZED_CCAP_SOURCE_SCORE10 | Copied the CCAP source identity from references and the original ccap-import event into the structured sources slot recognized by review scoring. |