f_2 (CultureMech:000149)

Overview

Applications

Algae cultivation
Phytoplankton culture
Microalgae research

Notes

Full recipe available at https://www.ccap.ac.uk/wp-content/uploads/MR_f2.pdf

Composition

flowchart LR
    CultureMech_000149["f_2"]
    ing0_Na_EDTA["Na EDTA"]
    CultureMech_000149 --> ing0_Na_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing0_Na_EDTA -.-> CHEBI_64734
    ing1_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_000149 --> ing1_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing1_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing2_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_000149 --> ing2_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing2_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing3_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_000149 --> ing3_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing3_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing4_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_000149 --> ing4_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing4_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing5_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_000149 --> ing5_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing5_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing6_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_000149 --> ing6_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing6_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing7_Cyanocobalamin__Vitamin_B__["Cyanocobalamin (Vitamin B )"]
    CultureMech_000149 --> ing7_Cyanocobalamin__Vitamin_B__
    CHEBI_17439["CHEBI:17439"]
    ing7_Cyanocobalamin__Vitamin_B__ -.-> CHEBI_17439
    ing8_Biotin["Biotin"]
    CultureMech_000149 --> ing8_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing8_Biotin -.-> CHEBI_15956
    ing9_Thiamine_HCl__Vitamin_B__["Thiamine HCl (Vitamin B )"]
    CultureMech_000149 --> ing9_Thiamine_HCl__Vitamin_B__
    CHEBI_49105["CHEBI:49105"]
    ing9_Thiamine_HCl__Vitamin_B__ -.-> CHEBI_49105
    ing10_Thiamine_HCl__B____["Thiamine HCl (B ) -"]
    CultureMech_000149 --> ing10_Thiamine_HCl__B____
    ing10_Thiamine_HCl__B____ -.-> CHEBI_49105
    ing11_Biotin["Biotin"]
    CultureMech_000149 --> ing11_Biotin
    ing11_Biotin -.-> CHEBI_15956
    ing12_Cyanocobalamin__B__["Cyanocobalamin (B )"]
    CultureMech_000149 --> ing12_Cyanocobalamin__B__
    ing12_Cyanocobalamin__B__ -.-> CHEBI_17439

Ingredients (13)

#	Ingredient	Concentration	Ontology	Notes
1	Na EDTA C10H14N2O8.2Na 336.208 g/mol	8.32 G_PER_L	CHEBI:64734 EDTA disodium salt (anhydrous)	From CCAP Medium MR_f2.pdf; Curated from CCAP Medium MR_f2.pdf specification
2	FeCl3 x 6 H2O Cl3Fe.6H2O 270.294 g/mol	6.30 G_PER_L	CHEBI:86254 iron trichloride hexahydrate	From CCAP Medium MR_f2.pdf; Curated from CCAP Medium MR_f2.pdf specification
3	CuSO4 x 5 H2O Cu.5H2O.O4S 249.684 g/mol	0.02 G_PER_L	CHEBI:31440 copper(II) sulfate pentahydrate	From CCAP Medium MR_f2.pdf; Curated from CCAP Medium MR_f2.pdf specification
4	ZnSO4 x 7 H2O 7H2O.O4S.Zn 287.558 g/mol	0.044 G_PER_L	CHEBI:32312 zinc sulfate heptahydrate	From CCAP Medium MR_f2.pdf; Curated from CCAP Medium MR_f2.pdf specification
5	CoCl2 x 6 H2O 2Cl.Co.6H2O 237.929 g/mol	0.02 G_PER_L	CHEBI:53503 cobalt chloride hexahydrate	From CCAP Medium MR_f2.pdf; Curated from CCAP Medium MR_f2.pdf specification
6	MnCl2 x 4 H2O Cl2Mn.4H2O 197.904 g/mol	0.36 G_PER_L	CHEBI:86368 manganese(II) chloride tetrahydrate	From CCAP Medium MR_f2.pdf; Curated from CCAP Medium MR_f2.pdf specification
7	Na2MoO4 x 2 H2O 2H2O.MoO4.2Na 241.946 g/mol	0.012 G_PER_L	CHEBI:75213 sodium molybdate dihydrate	From CCAP Medium MR_f2.pdf; Curated from CCAP Medium MR_f2.pdf specification
8	Cyanocobalamin (Vitamin B ) C63H88CoN14O14P 1355.388 g/mol	0.0005 G_PER_L	CHEBI:17439 cyanocob(III)alamin	From CCAP Medium MR_f2.pdf; Curated from CCAP Medium MR_f2.pdf specification
9	Biotin C10H16N2O3S 244.316 g/mol	0.0005 G_PER_L	CHEBI:15956 biotin	From CCAP Medium MR_f2.pdf; Curated from CCAP Medium MR_f2.pdf specification
10	Thiamine HCl (Vitamin B ) C12H17N4OS.Cl.HCl 337.276 g/mol	0.1 G_PER_L	CHEBI:49105 thiamine hydrochloride	From CCAP Medium MR_f2.pdf; Curated from CCAP Medium MR_f2.pdf specification
11	Thiamine HCl (B ) - C12H17N4OS.Cl.HCl 337.276 g/mol	0.1 G_PER_L	CHEBI:49105 thiamine hydrochloride	From CCAP Medium MR_f2.pdf; Curated from CCAP Medium MR_f2.pdf specification
12	Biotin C10H16N2O3S 244.316 g/mol	0.1 G_PER_L	CHEBI:15956 biotin	From CCAP Medium MR_f2.pdf; Curated from CCAP Medium MR_f2.pdf specification
13	Cyanocobalamin (B ) C63H88CoN14O14P 1355.388 g/mol	0.1 G_PER_L	CHEBI:17439 cyanocob(III)alamin	From CCAP Medium MR_f2.pdf; Curated from CCAP Medium MR_f2.pdf specification

Preparation

Bring to 1 L with filtered natural seawater.
Adjust pH to 8.0 with 1 M NaOH or 1 M HCl before autoclaving.
For an agar formulation, add 15 g/L Bacteriological Agar.
Autoclave at 15 psi for 15 minutes.

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-28T00:00:00+00:00	ccap-import	Imported from CCAP Culture Collection	Source ID: f2, PDF URL: https://www.ccap.ac.uk/wp-content/uploads/MR_f2.pdf
2026-02-03T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added placeholder ingredients for missing composition
2026-02-03T11:26:43.652903	quality-tagger-v1.0	Added data quality flag	Marked as incomplete_composition due to placeholder ingredients
2026-03-13T06:18:53.294610Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:000149
2026-03-17T19:45:04.240447+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "f/2" to "f_2" for consistent matching
2026-04-04T12:54:55.008396	batch_process_collection_media	EXPANDED_INGREDIENTS
2026-05-16T08:57:02.351243+00:00	migrate_data_quality_flags.py	MIGRATED_DATA_QUALITY_FLAGS	dict(4 keys) -> list(3 entries)
2026-05-16T09:02:56.545539+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=2; references.reference_id->reference=2; category->lowercase=1
2026-05-24T04:38:33.196459+00:00	backfill_source_environment.py	BACKFILLED_SOURCE_ENVIRONMENT	added=1 env(s) from CommunityMech
2026-08-25T00:00:00-07:00	repair_ccap_instruction_rows.py	REPAIRED_CCAP_INSTRUCTION_ROWS	Repaired preparation text and nested-recipe flattening against CCAP MR_f2.pdf. Moved process statements to preparation_steps and restored source-asserted components or constituent-medium references where applicable.
2026-09-13T00:00:00-07:00	repair_ccap_sources_score10.py	NORMALIZED_CCAP_SOURCE_SCORE10	Copied the CCAP source identity from references and the original ccap-import event into the structured sources slot recognized by review scoring.