Overview
- External ontology
- UTEX:cr1-s-diatom-medium (source link unavailable) — CR1-S Diatom Medium
- Applications
-
- Algae cultivation
- Phytoplankton culture
- Microalgae research
- Notes
- UTEX prepares CR1-S Diatom Medium tube-by-tube from 1 cc CR1 Soil, 11 mL dH2O, and 1 mL Sphagnum extract.
Composition
flowchart LR
CultureMech_000153["cr1_s_diatom_medium"]
ing0_CR1_Soil["CR1 Soil"]
CultureMech_000153 --> ing0_CR1_Soil
ing1_dH2O["dH2O"]
CultureMech_000153 --> ing1_dH2O
CHEBI_15377["CHEBI:15377"]
ing1_dH2O -.-> CHEBI_15377
ing2_Sphagnum_extract["Sphagnum extract"]
CultureMech_000153 --> ing2_Sphagnum_extract
Ingredients (3)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | CR1 Soil | 1 cc per 15 mL tube VARIABLE |
ENVO:00001998
soil
|
UTEX lists 1 cc CR1 Soil per 15 mL glass tube. |
| 2 |
dH2O
H2O 18.015 g/mol
|
916.667 ML_PER_L |
CHEBI:15377
water
|
Converted from 11 mL dH2O per 12 mL liquid fill. |
| 3 | Sphagnum extract | 83.333 ML_PER_L | Converted from 1 mL Sphagnum extract per 12 mL liquid fill. |
Preparation
- Add CR1 Soil, dH2O, and Sphagnum extract in order to 15 mL glass tubes.
- Process the soil and liquid mixture within 6 hours; autoclave for 15 minutes for routine maintenance, or pasteurize just above 98 C for 2 hours on three successive days.
- Allow tubes to gas-equilibrate for 2 days before use.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-28T00:00:00+00:00 | utex-import | Imported from UTEX Culture Collection | Source ID: cr1-s-diatom-medium, URL: https://utex.org/products/cr1-s-diatom-medium |
| 2026-03-13T06:18:53.310511Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:000153 |
| 2026-03-17T19:45:04.255429+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CR1-S Diatom Medium" to "cr1_s_diatom_medium" for consistent matching |
| 2026-05-16T09:02:56.442319+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1; references.reference_id->reference=2; category->lowercase=1 |
| 2026-05-16T09:11:01.593126+00:00 | migrate_preparation_steps.py | MIGRATED_PREPARATION_STEPS | instruction->action+description for 9 step(s) |
| 2026-08-30T00:58:31.480017+00:00 | repair_utex_ingredients.py | REPAIRED_UTEX_COMPOSITION | Restored 3 ingredient name(s) and amount(s) from a re-fetched UTEX capture. The original fetcher read fixed column positions, so the row ordinal was stored as the ingredient name, the component name as the amount, and the Amount column was never read; concentration was then filled in as G_PER_L regardless. Source: https://utex.org/pages/algal-culture-media, captured 2026-08-29T17:46:40.207779. |
| 2026-09-06T00:00:00-07:00 | repair_utex_cr1_s_modified_2x_score35.py | RESOLVED_UTEX_CR1_S_MODIFIED_2X_SCORE35 | UTEX prepares CR1-S Diatom Medium tube-by-tube from 1 cc CR1 Soil, 11 mL dH2O, and 1 mL Sphagnum extract. |