cr1_diatom_medium

CultureMech:000154 algae COMPLEX LIQUID
Original name: CR1 Diatom Medium

Overview

External ontology
UTEX:cr1-diatom-medium (source link unavailable) — CR1 Diatom Medium
Applications
  • Algae cultivation
  • Phytoplankton culture
  • Microalgae research
Notes
UTEX prepares CR1 Diatom Medium tube-by-tube from 1 cc CR1 Soil, 0.5 mg/12 mL MgCO3, and 12 mL dH2O.

Composition

flowchart LR
    CultureMech_000154["cr1_diatom_medium"]
    ing0_CR1_Soil["CR1 Soil"]
    CultureMech_000154 --> ing0_CR1_Soil
    ing1_MgCO3["MgCO3"]
    CultureMech_000154 --> ing1_MgCO3
    CHEBI_31793["CHEBI:31793"]
    ing1_MgCO3 -.-> CHEBI_31793
    ing2_dH2O["dH2O"]
    CultureMech_000154 --> ing2_dH2O
    CHEBI_15377["CHEBI:15377"]
    ing2_dH2O -.-> CHEBI_15377

Ingredients (3)

# Ingredient Concentration Ontology Notes
1 CR1 Soil 1 cc per 15 mL tube VARIABLE UTEX lists 1 cc CR1 Soil per 15 mL glass tube.
2 MgCO3
CO3.Mg 84.313 g/mol
MCIB CB486
0.5 MILLIMOLAR CHEBI:31793
magnesium carbonate
 UTEX lists 0.5 mg MgCO3 per 12 mL liquid fill, for 0.5 mM final.
3 dH2O
H2O 18.015 g/mol
1000 ML_PER_L CHEBI:15377
water
 Converted from 12 mL dH2O per 12 mL liquid fill.

Preparation

  1. Add CR1 Soil, MgCO3, and liquid medium in order to 15 mL glass tubes.
  2. Process the soil and liquid mixture within 6 hours; autoclave for 15 minutes for routine maintenance.
  3. Allow tubes to gas-equilibrate for 2 days before use.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-28T00:00:00+00:00 utex-import Imported from UTEX Culture Collection Source ID: cr1-diatom-medium, URL: https://utex.org/products/cr1-diatom-medium
2026-03-13T06:18:53.315052Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000154
2026-03-17T19:45:04.259545+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "CR1 Diatom Medium" to "cr1_diatom_medium" for consistent matching
2026-05-16T09:02:55.935927+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1; references.reference_id->reference=2; category->lowercase=1
2026-05-16T09:11:01.130413+00:00 migrate_preparation_steps.py MIGRATED_PREPARATION_STEPS instruction->action+description for 9 step(s)
2026-08-30T00:58:30.711491+00:00 repair_utex_ingredients.py REPAIRED_UTEX_COMPOSITION Restored 3 ingredient name(s) and amount(s) from a re-fetched UTEX capture. The original fetcher read fixed column positions, so the row ordinal was stored as the ingredient name, the component name as the amount, and the Amount column was never read; concentration was then filled in as G_PER_L regardless. Source: https://utex.org/pages/algal-culture-media, captured 2026-08-29T17:46:40.207779.
2026-09-06T00:00:00-07:00 repair_utex_cr1_diatom_score35.py RESOLVED_UTEX_CR1_DIATOM_SCORE35 UTEX prepares CR1 Diatom Medium tube-by-tube from 1 cc CR1 Soil, 0.5 mg/12 mL MgCO3, and 12 mL dH2O.
2026-09-06T00:00:00-07:00 repair_utex_cr1_s_modified_2x_score35.py LINKED_UTEX_CR1_S_MODIFIED_2X_VARIANTS Added or refreshed reciprocal parent-child link for algae/cr1_s_diatom_medium.yaml.