chus_medium

CultureMech:000155 algae COMPLEX LIQUID
Original name: Chu's Medium

Overview

External ontology
UTEX:chus-medium (source link unavailable) — Chu's Medium
Applications
  • Algae cultivation
  • Phytoplankton culture
  • Microalgae research
Notes
UTEX prepares Chu's Medium by adding 10 mL Chu Stock Solution and 0.17 mM NaHCO3 to sterile dH2O.

Composition

flowchart LR
    CultureMech_000155["chus_medium"]
    ing0_dH2O["dH2O"]
    CultureMech_000155 --> ing0_dH2O
    CHEBI_15377["CHEBI:15377"]
    ing0_dH2O -.-> CHEBI_15377
    ing1_NaHCO3["NaHCO3"]
    CultureMech_000155 --> ing1_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing1_NaHCO3 -.-> CHEBI_32139

Ingredients (2)

# Ingredient Concentration Ontology Notes
1 dH2O
H2O 18.015 g/mol
990 ML_PER_L CHEBI:15377
water
 UTEX lists 990 mL sterile dH2O per 1 L final medium.
2 NaHCO3
CHO3.Na 84.006 g/mol
Fisher S 233
0.17 MILLIMOLAR CHEBI:32139
sodium hydrogencarbonate
 UTEX lists 1 mL/L of a 1.26 g/90 mL NaHCO3 stock; final 0.17 mM.

Preparation

  1. Add 10 mL Chu Stock Solution to 990 mL sterile dH2O.
  2. Aseptically add sterile NaHCO3 to the medium.
  3. Store at refrigerator temperature.

Solutions

Chu Stock Solution

Dose: 10 ML_PER_L

Stock composition
IngredientConcentrationNotes
dH2O 1000 ML_PER_L UTEX brings Chu Stock Solution to 1 L with dH2O.
CaCl2 x 2 H2O 2.5 MILLIMOLAR UTEX lists this as 10 mL/L of a 3.67 g/100 mL stock.
MgSO4 x 7 H2O 1.5 MILLIMOLAR UTEX lists this as 10 mL/L of a 3.69 g/100 mL stock.
K2HPO4 0.5 MILLIMOLAR UTEX lists this as 10 mL/L of a 0.87 g/100 mL stock.
NaNO3 10 MILLIMOLAR UTEX lists this as 10 mL/L of an 8.5 g/100 mL stock.
Na2SiO3 x 9 H2O 1 MILLIMOLAR UTEX lists this as 10 mL/L of a 2.84 g/100 mL stock.
UTEX adds 10 mL Chu Stock Solution per liter of Chu's Medium.

Ferric Citrate Stock Solution for Chu

Dose: 100 ML_PER_L

Stock composition
IngredientConcentrationNotes
dH2O 1000 ML_PER_L UTEX brings Ferric Citrate Stock Solution for Chu to 100 mL with dH2O.
Citric Acid x H2O 33.5 G_PER_L Converted from 3.35 g/100 mL in the UTEX Ferric Citrate Stock Solution for Chu recipe.
Ferric citrate 33.5 G_PER_L Converted from 3.35 g/100 mL in the UTEX Ferric Citrate Stock Solution for Chu recipe.
UTEX adds 10 mL Ferric Citrate Stock Solution per 100 mL Chu Stock Solution.

Chu Micronutrient Solution

Dose: 100 ML_PER_L

Stock composition
IngredientConcentrationNotes
dH2O 1000 ML_PER_L UTEX brings Chu Micronutrient Solution to 1 L with dH2O.
CuSO4 x 5 H2O 0.02 G_PER_L UTEX lists 0.02 g/L in the Chu Micronutrient Solution recipe.
ZnSO4 x 7 H2O 0.044 G_PER_L UTEX lists 0.044 g/L in the Chu Micronutrient Solution recipe.
CoCl2 x 6 H2O 0.02 G_PER_L UTEX lists 0.02 g/L in the Chu Micronutrient Solution recipe.
MnCl2 x 4 H2O 0.012 G_PER_L UTEX lists 0.012 g/L in the Chu Micronutrient Solution recipe.
Na2MoO4 x 2 H2O 0.012 G_PER_L UTEX lists 0.012 g/L in the Chu Micronutrient Solution recipe.
H3BO3 0.62 G_PER_L UTEX lists 0.62 g/L in the Chu Micronutrient Solution recipe.
Na2EDTA x 2 H2O 0.05 G_PER_L UTEX lists 0.05 g/L in the Chu Micronutrient Solution recipe.
UTEX adds 10 mL Chu Micronutrient Solution per 100 mL Chu Stock Solution.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-28T00:00:00+00:00 utex-import Imported from UTEX Culture Collection Source ID: chus-medium, URL: https://utex.org/products/chus-medium
2026-03-13T06:18:53.319360Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000155
2026-03-17T19:45:04.263651+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Chu's Medium" to "chus_medium" for consistent matching
2026-05-16T09:02:56.432947+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1; references.reference_id->reference=2; category->lowercase=1
2026-05-16T09:11:01.584872+00:00 migrate_preparation_steps.py MIGRATED_PREPARATION_STEPS instruction->action+description for 9 step(s)
2026-08-30T00:58:31.466178+00:00 repair_utex_ingredients.py REPAIRED_UTEX_COMPOSITION Restored 2 ingredient name(s) and amount(s) from a re-fetched UTEX capture. The original fetcher read fixed column positions, so the row ordinal was stored as the ingredient name, the component name as the amount, and the Amount column was never read; concentration was then filled in as G_PER_L regardless. Source: https://utex.org/pages/algal-culture-media, captured 2026-08-29T17:46:40.207779.
2026-09-06T00:00:00-07:00 repair_utex_remaining_score50.py RESOLVED_UTEX_SCORE50_ALGAE_GRAPH UTEX prepares Chu's Medium by adding 10 mL Chu Stock Solution and 0.17 mM NaHCO3 to sterile dH2O.