cyanidium_medium

CultureMech:000157 algae COMPLEX LIQUID
Original name: Cyanidium Medium

Overview

External ontology
UTEX:cyanidium-medium (source link unavailable) — Cyanidium Medium
Applications
  • Algae cultivation
  • Phytoplankton culture
  • Microalgae research
pH
2.7
Notes
UTEX prepares Cyanidium Medium in 500 mL batches with ammonium sulfate, phosphate, magnesium sulfate, and Soilwater: GR+ Medium adjusted to pH 2.7.

Composition

flowchart LR
    CultureMech_000157["cyanidium_medium"]
    ing0_dH2O["dH2O"]
    CultureMech_000157 --> ing0_dH2O
    CHEBI_15377["CHEBI:15377"]
    ing0_dH2O -.-> CHEBI_15377
    ing1__NH4_2SO4["(NH4)2SO4"]
    CultureMech_000157 --> ing1__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing1__NH4_2SO4 -.-> CHEBI_62946
    ing2_K2HPO4["K2HPO4"]
    CultureMech_000157 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000157 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_Soilwater__GR__Medium["Soilwater: GR+ Medium"]
    CultureMech_000157 --> ing4_Soilwater__GR__Medium

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 dH2O
H2O 18.015 g/mol
1000 ML_PER_L CHEBI:15377
water
 UTEX starts the 500 mL Cyanidium recipe with approximately 200 mL dH2O.
2 (NH4)2SO4
2H4N.O4S 132.141 g/mol
Fisher A 702
3.78 MILLIMOLAR CHEBI:62946
ammonium sulfate
 UTEX lists 0.5 g/500 mL (NH4)2SO4, for 3.78 mM final.
3 K2HPO4
HO4P.2K 174.174 g/mol
Sigma P 3786
0.057 MILLIMOLAR CHEBI:131527
dipotassium hydrogen phosphate
 UTEX lists 0.01 g/500 mL K2HPO4, for 0.057 mM final.
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
Sigma 230391
0.041 MILLIMOLAR CHEBI:31795
magnesium sulfate heptahydrate
 UTEX lists 0.01 g/500 mL MgSO4 x 7 H2O, for 0.041 mM final.
5 Soilwater: GR+ Medium 30 ML_PER_L UTEX lists 30 mL prepared Soilwater: GR+ Medium per liter from 15 mL/500 mL.

Preparation

  1. To approximately 200 mL dH2O, add components in the listed order while stirring continuously.
  2. Adjust the medium to pH 2.7.
  3. Bring the total volume to 500 mL with dH2O.
  4. Cover and autoclave the medium.
  5. Store at refrigerator temperature.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-28T00:00:00+00:00 utex-import Imported from UTEX Culture Collection Source ID: cyanidium-medium, URL: https://utex.org/products/cyanidium-medium
2026-03-13T06:18:53.328999Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000157
2026-03-17T19:45:04.271901+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Cyanidium Medium" to "cyanidium_medium" for consistent matching
2026-05-16T09:02:55.945779+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1; references.reference_id->reference=2; category->lowercase=1
2026-05-16T09:11:01.140882+00:00 migrate_preparation_steps.py MIGRATED_PREPARATION_STEPS instruction->action+description for 9 step(s)
2026-08-30T00:58:30.724459+00:00 repair_utex_ingredients.py REPAIRED_UTEX_COMPOSITION Restored 4 ingredient name(s) and amount(s) from a re-fetched UTEX capture. The original fetcher read fixed column positions, so the row ordinal was stored as the ingredient name, the component name as the amount, and the Amount column was never read; concentration was then filled in as G_PER_L regardless. Source: https://utex.org/pages/algal-culture-media, captured 2026-08-29T17:46:40.207779.
2026-09-06T00:00:00-07:00 repair_utex_simple_score35.py RESOLVED_UTEX_SIMPLE_SCORE35 UTEX prepares Cyanidium Medium in 500 mL batches with ammonium sulfate, phosphate, magnesium sulfate, and Soilwater: GR+ Medium adjusted to pH 2.7.