cyanophycean_medium

CultureMech:000158 algae COMPLEX LIQUID
Original name: Cyanophycean Medium

Overview

External ontology
UTEX:cyanophycean-medium (source link unavailable) — Cyanophycean Medium
Applications
  • Algae cultivation
  • Phytoplankton culture
  • Microalgae research
Notes
UTEX prepares Cyanophycean Medium from dH2O, P-IV Metal Solution, KNO3, phosphate, and magnesium sulfate.

Composition

flowchart LR
    CultureMech_000158["cyanophycean_medium"]
    ing0_dH2O["dH2O"]
    CultureMech_000158 --> ing0_dH2O
    CHEBI_15377["CHEBI:15377"]
    ing0_dH2O -.-> CHEBI_15377
    ing1_KNO3["KNO3"]
    CultureMech_000158 --> ing1_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing1_KNO3 -.-> CHEBI_63043
    ing2_K2HPO4["K2HPO4"]
    CultureMech_000158 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000158 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795

Ingredients (4)

# Ingredient Concentration Ontology Notes
1 dH2O
H2O 18.015 g/mol
1000 ML_PER_L CHEBI:15377
water
 UTEX starts the 1 L Cyanophycean Medium recipe with approximately 975 mL dH2O.
2 KNO3
K.NO3 101.102 g/mol
Baker 3190
50 MILLIMOLAR CHEBI:63043
potassium nitrate
 UTEX lists 5 g/L KNO3, for 50 mM final.
3 K2HPO4
HO4P.2K 174.174 g/mol
Sigma P 3786
0.29 MILLIMOLAR CHEBI:131527
dipotassium hydrogen phosphate
 UTEX lists 0.05 g/L K2HPO4, for 0.29 mM final.
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
Sigma 230391
0.2 MILLIMOLAR CHEBI:31795
magnesium sulfate heptahydrate
 UTEX lists 0.05 g/L MgSO4 x 7 H2O, for 0.2 mM final.

Preparation

  1. To approximately 975 mL dH2O, add components in the listed order while stirring continuously.
  2. Bring the total volume to 1 L with dH2O.
  3. Cover and autoclave the medium.
  4. Store at refrigerator temperature.

Solutions

P-IV Metal Solution

Dose: 6 ML_PER_L

Stock composition
IngredientConcentrationNotes
dH2O 1000 ML_PER_L UTEX brings P-IV Metal Solution to 1 L with dH2O.
Na2EDTA x 2 H2O 0.75 G_PER_L UTEX lists 0.75 g/L Na2EDTA x 2 H2O, 2 mM final, and dissolves it fully before the other P-IV components.
FeCl3 x 6 H2O 0.097 G_PER_L UTEX lists 0.097 g/L FeCl3 x 6 H2O, for 0.36 mM final.
MnCl2 x 4 H2O 0.041 G_PER_L UTEX lists 0.041 g/L MnCl2 x 4 H2O, for 0.21 mM final.
ZnCl2 0.005 G_PER_L UTEX lists 0.005 g/L ZnCl2, for 0.037 mM final.
CoCl2 x 6 H2O 0.002 G_PER_L UTEX lists 0.002 g/L CoCl2 x 6 H2O, for 0.0084 mM final.
Na2MoO4 x 2 H2O 0.004 G_PER_L UTEX lists 0.004 g/L Na2MoO4 x 2 H2O, for 0.017 mM final.
UTEX adds 6 mL P-IV Metal Solution per liter.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-28T00:00:00+00:00 utex-import Imported from UTEX Culture Collection Source ID: cyanophycean-medium, URL: https://utex.org/products/cyanophycean-medium
2026-03-13T06:18:53.333670Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000158
2026-03-17T19:45:04.276213+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Cyanophycean Medium" to "cyanophycean_medium" for consistent matching
2026-05-16T09:02:55.950518+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1; references.reference_id->reference=2; category->lowercase=1
2026-05-16T09:11:01.146477+00:00 migrate_preparation_steps.py MIGRATED_PREPARATION_STEPS instruction->action+description for 9 step(s)
2026-08-30T00:58:30.731392+00:00 repair_utex_ingredients.py REPAIRED_UTEX_COMPOSITION Restored 4 ingredient name(s) and amount(s) from a re-fetched UTEX capture. The original fetcher read fixed column positions, so the row ordinal was stored as the ingredient name, the component name as the amount, and the Amount column was never read; concentration was then filled in as G_PER_L regardless. Source: https://utex.org/pages/algal-culture-media, captured 2026-08-29T17:46:40.207779.
2026-09-06T00:00:00-07:00 repair_utex_simple_score35.py RESOLVED_UTEX_SIMPLE_SCORE35 UTEX prepares Cyanophycean Medium from dH2O, P-IV Metal Solution, KNO3, phosphate, and magnesium sulfate.