dyv_medium

CultureMech:000160 algae COMPLEX LIQUID
Original name: DYV Medium

Overview

External ontology
UTEX:dyv-medium (source link unavailable) — DYV Medium
Applications
  • Algae cultivation
  • Phytoplankton culture
  • Microalgae research
pH
6.7
Notes
UTEX prepares DYV Medium as DYIII Medium supplemented with 1 mL/L DYV Metal Solution.

Composition

flowchart LR
    CultureMech_000160["dyv_medium"]
    ing0_dH2O["dH2O"]
    CultureMech_000160 --> ing0_dH2O
    CHEBI_15377["CHEBI:15377"]
    ing0_dH2O -.-> CHEBI_15377
    ing1_MES["MES"]
    CultureMech_000160 --> ing1_MES
    CHEBI_39005["CHEBI:39005"]
    ing1_MES -.-> CHEBI_39005
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_000160 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_000160 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_Na2Glycerophosphate_x_5_H2O["Na2Glycerophosphate x 5 H2O"]
    CultureMech_000160 --> ing4_Na2Glycerophosphate_x_5_H2O
    CHEBI_15978["CHEBI:15978"]
    ing4_Na2Glycerophosphate_x_5_H2O -.-> CHEBI_15978
    ing5_NaNO3["NaNO3"]
    CultureMech_000160 --> ing5_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing5_NaNO3 -.-> CHEBI_63005
    ing6_Na2SiO3_x_9_H2O["Na2SiO3 x 9 H2O"]
    CultureMech_000160 --> ing6_Na2SiO3_x_9_H2O
    CHEBI_132108["CHEBI:132108"]
    ing6_Na2SiO3_x_9_H2O -.-> CHEBI_132108
    ing7_NH4NO3["NH4NO3"]
    CultureMech_000160 --> ing7_NH4NO3
    CHEBI_63038["CHEBI:63038"]
    ing7_NH4NO3 -.-> CHEBI_63038
    ing8_KCl["KCl"]
    CultureMech_000160 --> ing8_KCl
    CHEBI_32588["CHEBI:32588"]
    ing8_KCl -.-> CHEBI_32588

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 dH2O
H2O 18.015 g/mol
1000 ML_PER_L CHEBI:15377
water
 UTEX starts the 1 L DYV Medium recipe with approximately 950 mL dH2O.
2 MES
C6H13NO4S 195.24 g/mol
10 MILLIMOLAR CHEBI:39005
2-(N-morpholino)ethanesulfonic acid
 UTEX lists 1.95 g/L MES, for 10 mM final.
3 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.14 MILLIMOLAR CHEBI:86158
calcium chloride dihydrate
 UTEX adds 1 mL/L of a 2 g/100 mL CaCl2 x 2 H2O stock.
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.3 MILLIMOLAR CHEBI:31795
magnesium sulfate heptahydrate
 UTEX adds 1 mL/L of a 7.4 g/100 mL MgSO4 x 7 H2O stock.
5 Na2Glycerophosphate x 5 H2O
C3H9O6P 172.073 g/mol
0.03 MILLIMOLAR CHEBI:15978
sn-glycerol 3-phosphate
 UTEX adds 1 mL/L of a 1 g/100 mL Na2Glycerophosphate x 5 H2O stock.
6 NaNO3
NO3.Na 84.994 g/mol
0.24 MILLIMOLAR CHEBI:63005
sodium nitrate
 UTEX adds 1 mL/L of a 2 g/100 mL NaNO3 stock.
7 Na2SiO3 x 9 H2O
9H2O.2Na.O3Si 284.198 g/mol
0.05 MILLIMOLAR CHEBI:132108
sodium silicate nonahydrate
 UTEX adds 1 mL/L of a 1.5 g/100 mL Na2SiO3 x 9 H2O stock.
8 NH4NO3
H4N.NO3 80.043 g/mol
0.12 MILLIMOLAR CHEBI:63038
ammonium nitrate
 UTEX adds 1 mL/L of a 1 g/100 mL NH4NO3 stock.
9 KCl
Cl.K 74.551 g/mol
0.13 MILLIMOLAR CHEBI:32588
potassium chloride
 UTEX adds 1 mL/L of a 1 g/100 mL KCl stock.

Preparation

  1. To approximately 950 mL dH2O, add all non-vitamin components in the listed order while stirring continuously.
  2. Adjust the medium to pH 6.7.
  3. Bring the total volume to 1 L with dH2O.
  4. Cover and autoclave the medium.
  5. When cooled, add the sterile vitamin stocks.
  6. Store at refrigerator temperature.

Solutions

MWC Metal Solution

Dose: 10 ML_PER_L

Stock composition
IngredientConcentrationNotes
dH2O 1000 ML_PER_L UTEX brings MWC Metal Solution to 1 L with dH2O.
Na2EDTA x 2 H2O 0.44 G_PER_L UTEX lists 0.44 g/L Na2EDTA x 2 H2O.
H3BO3 0.097 G_PER_L UTEX lists 0.097 g/L H3BO3.
FeCl3 x 6 H2O 0.315 G_PER_L UTEX lists 0.315 g/L FeCl3 x 6 H2O.
MnCl2 x 4 H2O 0.018 G_PER_L UTEX lists 0.018 g/L MnCl2 x 4 H2O.
ZnSO4 x 7 H2O 0.002 G_PER_L UTEX lists 0.002 g/L ZnSO4 x 7 H2O.
CoCl2 x 6 H2O 0.001 G_PER_L UTEX lists 0.001 g/L CoCl2 x 6 H2O.
Na2MoO4 x 2 H2O 0.0006 G_PER_L UTEX lists 0.0006 g/L Na2MoO4 x 2 H2O.
UTEX adds 10 mL MWC Metal Solution per liter.

DYV Metal Solution

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
dH2O 1000 ML_PER_L UTEX brings DYV Metal Solution to 1 L with dH2O.
Na3VO4 0.002 G_PER_L UTEX lists 0.002 g/L Na3VO4.
H2SeO3 0.004 G_PER_L UTEX lists 0.004 g/L H2SeO3.
UTEX adds 1 mL DYV Metal Solution per liter.

Vitamin B12

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
dH2O 1000 ML_PER_L UTEX prepares the Vitamin B12 stock in 200 mL HEPES buffer.
HEPES buffer pH 7.8 12 G_PER_L Converted from 2.4 g/200 mL in the UTEX Vitamin B12 stock.
Vitamin B12 0.135 G_PER_L Converted from 0.027 g/200 mL in the UTEX Vitamin B12 stock.
UTEX adds 1 mL Vitamin B12 stock per liter.

Biotin Vitamin Solution

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
dH2O 1000 ML_PER_L UTEX prepares the biotin stock in 200 mL HEPES buffer.
HEPES buffer pH 7.8 12 G_PER_L Converted from 2.4 g/200 mL in the UTEX biotin stock.
Biotin 0.025 G_PER_L Converted from 0.005 g/200 mL in the UTEX biotin stock.
UTEX adds 1 mL Biotin Vitamin Solution per liter.

Thiamine Vitamin Solution

Dose: 1 ML_PER_L

Stock composition
IngredientConcentrationNotes
dH2O 1000 ML_PER_L UTEX prepares the thiamine stock in 200 mL HEPES buffer.
HEPES buffer pH 7.8 12 G_PER_L Converted from 2.4 g/200 mL in the UTEX thiamine stock.
Thiamine 0.335 G_PER_L Converted from 0.067 g/200 mL in the UTEX thiamine stock.
UTEX adds 1 mL Thiamine Vitamin Solution per liter.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-28T00:00:00+00:00 utex-import Imported from UTEX Culture Collection Source ID: dyv-medium, URL: https://utex.org/products/dyv-medium
2026-03-13T06:18:53.347005Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:000160
2026-03-17T19:45:04.289119+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "DYV Medium" to "dyv_medium" for consistent matching
2026-05-16T09:02:55.963362+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1; references.reference_id->reference=2; category->lowercase=1
2026-05-16T09:11:01.160435+00:00 migrate_preparation_steps.py MIGRATED_PREPARATION_STEPS instruction->action+description for 9 step(s)
2026-08-30T00:58:30.749082+00:00 repair_utex_ingredients.py REPAIRED_UTEX_COMPOSITION Restored 13 ingredient name(s) and amount(s) from a re-fetched UTEX capture. The original fetcher read fixed column positions, so the row ordinal was stored as the ingredient name, the component name as the amount, and the Amount column was never read; concentration was then filled in as G_PER_L regardless. Source: https://utex.org/pages/algal-culture-media, captured 2026-08-29T17:46:40.207779.
2026-09-06T00:00:00-07:00 repair_utex_dyiii_dyv_score35.py RESOLVED_UTEX_DYIII_DYV_SCORE35 UTEX prepares DYV Medium as DYIII Medium supplemented with 1 mL/L DYV Metal Solution.